Enzymatic Ligation Creates Discrete Multi-Nanoparticle Building Blocks for Self-Assembly

Journal Article · Tue May 27 00:00:00 EDT 2008 · Journal of the American Chemical Society

OSTI ID:941057

Claridge, Shelley A; Mastroianni, Alexander J; Au, Yeung B; Liang, Huiyang W; Micheel, Christine M; Frechet, Jean M.J.; Alivisatos, A Paul

Enzymatic ligation of discrete nanoparticle?DNA conjugates creates nanoparticle dimer and trimer structures in which the nanoparticles are linked by single-stranded DNA, rather than double-stranded DNA as in previous experiments. Ligation is verified by agarose gel and small-angle X-ray scattering. This capability is utilized in two ways: first to create a new class of multiparticle building blocks for nanoscale self-assembly; second to develop a system which can amplify a population of discrete nanoparticle assemblies.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: Materials Sciences Division
• DOE Contract Number: DE-AC02-05CH11231
• OSTI ID: 941057
• Report Number(s): LBNL-1171E; JACSAT; TRN: US200824%%521
• Journal Information: Journal of the American Chemical Society, Vol. 130; Related Information: Journal Publication Date: July 23, 2008; ISSN 0002-7863
• Country of Publication: United States
• Language: English

Related Subjects

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36
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DIMERS
DNA
SCATTERING
nanocrystal
nanoparticle
gold
DNA
ligase
bioconjugate
enzyme
amplification