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Title: Recent Developments in the Site-Specific Immobilization of Proteins onto Solid Supports

Abstract

Immobilization of proteins onto surfaces is of great importance in numerous applications, including protein analysis, drug screening, and medical diagnostics, among others. The success of all these technologies relies on the immobilization technique employed to attach a protein to the corresponding surface. Non-specific physical adsorption or chemical cross-linking with appropriate surfaces results in the immobilization of the protein in random orientations. Site-specific covalent attachment, on the other hand, leads to molecules being arranged in a definite, orderly fashion and allows the use of spacers and linkers to help minimize steric hindrances between the protein and the surface. The present work reviews the latest chemical and biochemical developments for the site-specific covalent attachment of proteins onto solid supports.

Authors:
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
940515
Report Number(s):
UCRL-JRNL-228513
Journal ID: ISSN 0006-3525; BIPMAA; TRN: US200824%%78
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biopolymers, vol. 90, no. 3, January 1, 2008, pp. 450-458; Journal Volume: 90; Journal Issue: 3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ADSORPTION; CROSS-LINKING; PROTEINS; SPACERS

Citation Formats

Camarero, J A. Recent Developments in the Site-Specific Immobilization of Proteins onto Solid Supports. United States: N. p., 2007. Web.
Camarero, J A. Recent Developments in the Site-Specific Immobilization of Proteins onto Solid Supports. United States.
Camarero, J A. Wed . "Recent Developments in the Site-Specific Immobilization of Proteins onto Solid Supports". United States. doi:. https://www.osti.gov/servlets/purl/940515.
@article{osti_940515,
title = {Recent Developments in the Site-Specific Immobilization of Proteins onto Solid Supports},
author = {Camarero, J A},
abstractNote = {Immobilization of proteins onto surfaces is of great importance in numerous applications, including protein analysis, drug screening, and medical diagnostics, among others. The success of all these technologies relies on the immobilization technique employed to attach a protein to the corresponding surface. Non-specific physical adsorption or chemical cross-linking with appropriate surfaces results in the immobilization of the protein in random orientations. Site-specific covalent attachment, on the other hand, leads to molecules being arranged in a definite, orderly fashion and allows the use of spacers and linkers to help minimize steric hindrances between the protein and the surface. The present work reviews the latest chemical and biochemical developments for the site-specific covalent attachment of proteins onto solid supports.},
doi = {},
journal = {Biopolymers, vol. 90, no. 3, January 1, 2008, pp. 450-458},
number = 3,
volume = 90,
place = {United States},
year = {Wed Feb 21 00:00:00 EST 2007},
month = {Wed Feb 21 00:00:00 EST 2007}
}