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Title: Using Allele-Specific PCR with Molecular Beams as a Means for Genotyping the Diallelic Indels

Abstract

The first Specific Aim for this grant was to identify and characterize an average of 500 human insertion/deletion polymorphisms per grant year (1500 total). This task was carried out entirely at MMRF. They substantially exceeded this goal by confirming about 2,300 diallelic indels. Complete characterization information for these polymorphisms is available from the Marshfield web site. A manuscript describing results for the first 2,000 diallelic indels was published earlier this year in the American Journal of Human Genetics. The Second Specific Aim of the grant was to investigate and develop improved methods for analysis of diallelic polymorphisms using miniaturized DNA arrays. The initial genotyping technology efforts focused on various hybridization and extension protocols with oligo arrays on flow-through channel glass. Channel glass is a porous material that permits reagents to be passed through the arrays. They devoted roughly 19 months at the beginning of the grant in pursuit of this methodology, but for various technological reasons, progress was limited.

Authors:
;  [1]
  1. (Marshfield Medical Research Foundation)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
940386
Report Number(s):
ORNL98-0537
TRN: US200902%%231
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; DNA; GENETICS; GLASS; HYBRIDIZATION; MOLECULAR BEAMS; POROUS MATERIALS

Citation Formats

Doktycz, M.J., and Weber, J.L. Using Allele-Specific PCR with Molecular Beams as a Means for Genotyping the Diallelic Indels. United States: N. p., 2000. Web. doi:10.2172/940386.
Doktycz, M.J., & Weber, J.L. Using Allele-Specific PCR with Molecular Beams as a Means for Genotyping the Diallelic Indels. United States. doi:10.2172/940386.
Doktycz, M.J., and Weber, J.L. Thu . "Using Allele-Specific PCR with Molecular Beams as a Means for Genotyping the Diallelic Indels". United States. doi:10.2172/940386. https://www.osti.gov/servlets/purl/940386.
@article{osti_940386,
title = {Using Allele-Specific PCR with Molecular Beams as a Means for Genotyping the Diallelic Indels},
author = {Doktycz, M.J. and Weber, J.L.},
abstractNote = {The first Specific Aim for this grant was to identify and characterize an average of 500 human insertion/deletion polymorphisms per grant year (1500 total). This task was carried out entirely at MMRF. They substantially exceeded this goal by confirming about 2,300 diallelic indels. Complete characterization information for these polymorphisms is available from the Marshfield web site. A manuscript describing results for the first 2,000 diallelic indels was published earlier this year in the American Journal of Human Genetics. The Second Specific Aim of the grant was to investigate and develop improved methods for analysis of diallelic polymorphisms using miniaturized DNA arrays. The initial genotyping technology efforts focused on various hybridization and extension protocols with oligo arrays on flow-through channel glass. Channel glass is a porous material that permits reagents to be passed through the arrays. They devoted roughly 19 months at the beginning of the grant in pursuit of this methodology, but for various technological reasons, progress was limited.},
doi = {10.2172/940386},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {6}
}