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Title: Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry.

Abstract

A novel analytical method based on laser desorption postionization mass spectrometry (LDPI-MS) was developed to investigate the competence and sporulation factor-a pentapeptide of amino acid sequence ERGMT-within intact Bacillus subtilis biofilms. Derivatization of the neat ERGMT peptide with quinoline- and anthracene-based tags was separately used to lower the peptide ionization potential and permit direct ionization by 7.87-eV vacuum ultraviolet radiation. The techniques of mass shifting and selective ionization of the derivatized peptide were combined here to permit detection of ERGMT peptide within intact biofilms by LDPI-MS, without any prior extraction or chromatographic separation. Finally, imaging MS specific to the derivatized peptide was demonstrated on an intact biofilm using LDPI-MS. The presence of ERGMT in the biofilms was verified by bulk extraction/LC-MS. However, MALDI imaging MS analyses were unable to detect ERGMT within intact biofilms.

Authors:
; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
939554
Report Number(s):
ANL/BIO/JA-57364
Journal ID: ISSN 0003-2700; ANCHAM; TRN: US200823%%265
DOE Contract Number:
DE-AC02-06CH11357
Resource Type:
Journal Article
Resource Relation:
Journal Name: Anal. Chem.; Journal Volume: 79; Journal Issue: 2 ; 2007
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMINO ACID SEQUENCE; BACILLUS SUBTILIS; DERIVATIZATION; DESORPTION; DETECTION; FAR ULTRAVIOLET RADIATION; IONIZATION; IONIZATION POTENTIAL; LASERS; MASS SPECTROSCOPY; PEPTIDES

Citation Formats

Edirisinghe, P. D., Moore, J. F., Skinner-Nemec, K. A., Lindberg, C., Giometti, C. S., Veryovkin, I. V., Hunt, J. E., Pellin, M. J., Hanley, L., Biosciences Division, Univ. of Illinois at Chicago, and MassThink. Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry.. United States: N. p., 2007. Web. doi:10.1021/ac0615605.
Edirisinghe, P. D., Moore, J. F., Skinner-Nemec, K. A., Lindberg, C., Giometti, C. S., Veryovkin, I. V., Hunt, J. E., Pellin, M. J., Hanley, L., Biosciences Division, Univ. of Illinois at Chicago, & MassThink. Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry.. United States. doi:10.1021/ac0615605.
Edirisinghe, P. D., Moore, J. F., Skinner-Nemec, K. A., Lindberg, C., Giometti, C. S., Veryovkin, I. V., Hunt, J. E., Pellin, M. J., Hanley, L., Biosciences Division, Univ. of Illinois at Chicago, and MassThink. Mon . "Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry.". United States. doi:10.1021/ac0615605.
@article{osti_939554,
title = {Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry.},
author = {Edirisinghe, P. D. and Moore, J. F. and Skinner-Nemec, K. A. and Lindberg, C. and Giometti, C. S. and Veryovkin, I. V. and Hunt, J. E. and Pellin, M. J. and Hanley, L. and Biosciences Division and Univ. of Illinois at Chicago and MassThink},
abstractNote = {A novel analytical method based on laser desorption postionization mass spectrometry (LDPI-MS) was developed to investigate the competence and sporulation factor-a pentapeptide of amino acid sequence ERGMT-within intact Bacillus subtilis biofilms. Derivatization of the neat ERGMT peptide with quinoline- and anthracene-based tags was separately used to lower the peptide ionization potential and permit direct ionization by 7.87-eV vacuum ultraviolet radiation. The techniques of mass shifting and selective ionization of the derivatized peptide were combined here to permit detection of ERGMT peptide within intact biofilms by LDPI-MS, without any prior extraction or chromatographic separation. Finally, imaging MS specific to the derivatized peptide was demonstrated on an intact biofilm using LDPI-MS. The presence of ERGMT in the biofilms was verified by bulk extraction/LC-MS. However, MALDI imaging MS analyses were unable to detect ERGMT within intact biofilms.},
doi = {10.1021/ac0615605},
journal = {Anal. Chem.},
number = 2 ; 2007,
volume = 79,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}