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Title: Is Increased Low-dose somatic Radiosensitivity Associated with Increased Transgenerational Germline Mutation

Abstract

Using single-molecule polymerase chain reaction, the frequency of spontaneous and radiation-induced mutation at an expanded simple tandem repeat (ESTR) locus was studied in DNA samples extracted from sperm and bone marrow of Atm knockout (Atm+/–) heterozygous male mice. The frequency of spontaneous mutation in sperm and bone marrow in Atm+/– males did not significantly differ from that in wild-type BALB/c mice. Acute gamma-ray exposure did not affect ESTR mutation frequency in bone marrow and resulted in similar increases in sperm samples taken from Atm+/– and BALB/c males. Taken together, these results suggest that the Atm haploinsufficiency analyzed in our study does not affect spontaneous and radiation-induced ESTR mutation frequency in mice.

Authors:
Publication Date:
Research Org.:
Columbia University Medical Center, Center for Radiological Research
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
938982
Report Number(s):
DOE/ER/63631
TRN: US0902919
DOE Contract Number:  
FG02-03ER63631
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
61 RADIATION PROTECTION AND DOSIMETRY; BONE MARROW; DNA; MALES; MICE; MUTATION FREQUENCY; MUTATIONS; POLYMERASE CHAIN REACTION; RADIOSENSITIVITY; SPERMATOZOA; SPONTANEOUS MUTATIONS; Low-Dose, Radiosensitivity

Citation Formats

Brenner, David J. Is Increased Low-dose somatic Radiosensitivity Associated with Increased Transgenerational Germline Mutation. United States: N. p., 2008. Web. doi:10.2172/938982.
Brenner, David J. Is Increased Low-dose somatic Radiosensitivity Associated with Increased Transgenerational Germline Mutation. United States. doi:10.2172/938982.
Brenner, David J. Thu . "Is Increased Low-dose somatic Radiosensitivity Associated with Increased Transgenerational Germline Mutation". United States. doi:10.2172/938982. https://www.osti.gov/servlets/purl/938982.
@article{osti_938982,
title = {Is Increased Low-dose somatic Radiosensitivity Associated with Increased Transgenerational Germline Mutation},
author = {Brenner, David J.},
abstractNote = {Using single-molecule polymerase chain reaction, the frequency of spontaneous and radiation-induced mutation at an expanded simple tandem repeat (ESTR) locus was studied in DNA samples extracted from sperm and bone marrow of Atm knockout (Atm+/–) heterozygous male mice. The frequency of spontaneous mutation in sperm and bone marrow in Atm+/– males did not significantly differ from that in wild-type BALB/c mice. Acute gamma-ray exposure did not affect ESTR mutation frequency in bone marrow and resulted in similar increases in sperm samples taken from Atm+/– and BALB/c males. Taken together, these results suggest that the Atm haploinsufficiency analyzed in our study does not affect spontaneous and radiation-induced ESTR mutation frequency in mice.},
doi = {10.2172/938982},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {10}
}