skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Elastomeric Microchip Electrospray Emitter for Stable Cone-Jet Mode Operation in the Nanoflow Regime.

Abstract

Despite widespread interest in applying lab-on-a-chip technologies to mass spectrometry (MS)-based analyses, the coupling of microfluidics to electrospray ionization (ESI)-MS remains challenging. We report a robust, integrated poly(dimethylsiloxane) microchip interface for ESI-MS using simple and widely accessible microfabrication procedures. The interface uses an auxiliary channel to provide electrical contact in the Taylor cone of the electrospray without sample loss or dilution. The electric field at the channel terminus is enhanced by two vertical cuts that cause the interface to taper to a line rather than to a point, and the formation of small Taylor cones at the channel exit ensures sub-nL post-column dead volumes. While comparable ESI-MS sensitivities were achieved using both microchip and conventional fused silica capillary emitters, stable cone-jet mode electrospray could be established over a far broader range of flow rates (from 50–1000 nL/min) and applied potentials using the microchip emitters. This special feature of the microchip emitter should minimize the fine tuning required for electrospray optimization and make the stable electrospray more resistant to external perturbations.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
937029
Report Number(s):
PNNL-SA-58551
KP1601010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry, 80(10):3824-3831
Additional Journal Information:
Journal Volume: 80; Journal Issue: 10
Country of Publication:
United States
Language:
English

Citation Formats

Kelly, Ryan T., Tang, Keqi, Irimia, Daniel, Toner, Mehmet, and Smith, Richard D. Elastomeric Microchip Electrospray Emitter for Stable Cone-Jet Mode Operation in the Nanoflow Regime.. United States: N. p., 2008. Web. doi:10.1021/ac8000786.
Kelly, Ryan T., Tang, Keqi, Irimia, Daniel, Toner, Mehmet, & Smith, Richard D. Elastomeric Microchip Electrospray Emitter for Stable Cone-Jet Mode Operation in the Nanoflow Regime.. United States. https://doi.org/10.1021/ac8000786
Kelly, Ryan T., Tang, Keqi, Irimia, Daniel, Toner, Mehmet, and Smith, Richard D. Thu . "Elastomeric Microchip Electrospray Emitter for Stable Cone-Jet Mode Operation in the Nanoflow Regime.". United States. https://doi.org/10.1021/ac8000786.
@article{osti_937029,
title = {Elastomeric Microchip Electrospray Emitter for Stable Cone-Jet Mode Operation in the Nanoflow Regime.},
author = {Kelly, Ryan T. and Tang, Keqi and Irimia, Daniel and Toner, Mehmet and Smith, Richard D.},
abstractNote = {Despite widespread interest in applying lab-on-a-chip technologies to mass spectrometry (MS)-based analyses, the coupling of microfluidics to electrospray ionization (ESI)-MS remains challenging. We report a robust, integrated poly(dimethylsiloxane) microchip interface for ESI-MS using simple and widely accessible microfabrication procedures. The interface uses an auxiliary channel to provide electrical contact in the Taylor cone of the electrospray without sample loss or dilution. The electric field at the channel terminus is enhanced by two vertical cuts that cause the interface to taper to a line rather than to a point, and the formation of small Taylor cones at the channel exit ensures sub-nL post-column dead volumes. While comparable ESI-MS sensitivities were achieved using both microchip and conventional fused silica capillary emitters, stable cone-jet mode electrospray could be established over a far broader range of flow rates (from 50–1000 nL/min) and applied potentials using the microchip emitters. This special feature of the microchip emitter should minimize the fine tuning required for electrospray optimization and make the stable electrospray more resistant to external perturbations.},
doi = {10.1021/ac8000786},
url = {https://www.osti.gov/biblio/937029}, journal = {Analytical Chemistry, 80(10):3824-3831},
number = 10,
volume = 80,
place = {United States},
year = {2008},
month = {5}
}