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Title: Integrating Membrane Transport with Male Gametophyte Development and Function through Transcriptomics.

Abstract

Male fertility depends on the proper development of the male gametophyte, successful pollen germination, tube growth and delivery of the sperm cells to the ovule. Previous studies have shown that nutrients like boron, and ion gradients or currents of Ca2+, H+, and K+ are critical for pollen tube growth. However, the molecular identities of transporters mediating these fluxes are mostly unknown. As a first step to integrate transport with pollen development and function, a genome-wide analysis of transporter genes expressed in the male gametophyte at four developmental stages was conducted. About 1269 genes encoding classified transporters were collected from the Arabidopsis thaliana genome. Of 757 transporter genes expressed in pollen, 16% or 124 genes, including AHA6, CNGC18, TIP1.3 and CHX08, are specifically or preferentially expressed relative to sporophytic tissues. Some genes are highly expressed in microspores and bicellular pollen (COPT3, STP2, OPT9); while others are activated only in tricellular or mature pollen (STP11, LHT7). Analyses of entire gene families showed that a subset of genes, including those expressed in sporophytic tissues, were developmentally-regulated during pollen maturation. Early and late expression patterns revealed by transcriptome analysis are supported by promoter::GUS analyses of CHX genes and by other methods. Recent genetic studiesmore » based on a few transporters, including plasma membrane H+ pump AHA3, Ca2+ pump ACA9, and K+ channel SPIK, further support the expression patterns and the inferred functions revealed by our analyses. Thus, revealing the distinct expression patterns of specific transporters and unknown polytopic proteins during microgametogenesis provides new insights for strategic mutant analyses necessary to integrate the roles of transporters and potential receptors with male gametophyte development.« less

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
University of Maryland, College Park, MD
Sponsoring Org.:
USDOE - Office of Energy Research (ER)
OSTI Identifier:
932549
Report Number(s):
DOE/ER/20200-2
DOE Contract Number:  
FG02-95ER20200
Resource Type:
Journal Article
Resource Relation:
Journal Name: Plant Physiology; Journal Volume: 140
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Systems biology, membrane transport, Arabidopsis thaliana, genomics, pollen, transcriptome

Citation Formats

Bock KW, D Honys, JM. Ward, S Padmanaban, EP Nawrocki, KD Hirschi, D Twell, and H Sze. Integrating Membrane Transport with Male Gametophyte Development and Function through Transcriptomics.. United States: N. p., 2006. Web. doi:10.1104/pp.105.074708.
Bock KW, D Honys, JM. Ward, S Padmanaban, EP Nawrocki, KD Hirschi, D Twell, & H Sze. Integrating Membrane Transport with Male Gametophyte Development and Function through Transcriptomics.. United States. doi:10.1104/pp.105.074708.
Bock KW, D Honys, JM. Ward, S Padmanaban, EP Nawrocki, KD Hirschi, D Twell, and H Sze. Sun . "Integrating Membrane Transport with Male Gametophyte Development and Function through Transcriptomics.". United States. doi:10.1104/pp.105.074708.
@article{osti_932549,
title = {Integrating Membrane Transport with Male Gametophyte Development and Function through Transcriptomics.},
author = {Bock KW and D Honys and JM. Ward and S Padmanaban and EP Nawrocki and KD Hirschi and D Twell and H Sze},
abstractNote = {Male fertility depends on the proper development of the male gametophyte, successful pollen germination, tube growth and delivery of the sperm cells to the ovule. Previous studies have shown that nutrients like boron, and ion gradients or currents of Ca2+, H+, and K+ are critical for pollen tube growth. However, the molecular identities of transporters mediating these fluxes are mostly unknown. As a first step to integrate transport with pollen development and function, a genome-wide analysis of transporter genes expressed in the male gametophyte at four developmental stages was conducted. About 1269 genes encoding classified transporters were collected from the Arabidopsis thaliana genome. Of 757 transporter genes expressed in pollen, 16% or 124 genes, including AHA6, CNGC18, TIP1.3 and CHX08, are specifically or preferentially expressed relative to sporophytic tissues. Some genes are highly expressed in microspores and bicellular pollen (COPT3, STP2, OPT9); while others are activated only in tricellular or mature pollen (STP11, LHT7). Analyses of entire gene families showed that a subset of genes, including those expressed in sporophytic tissues, were developmentally-regulated during pollen maturation. Early and late expression patterns revealed by transcriptome analysis are supported by promoter::GUS analyses of CHX genes and by other methods. Recent genetic studies based on a few transporters, including plasma membrane H+ pump AHA3, Ca2+ pump ACA9, and K+ channel SPIK, further support the expression patterns and the inferred functions revealed by our analyses. Thus, revealing the distinct expression patterns of specific transporters and unknown polytopic proteins during microgametogenesis provides new insights for strategic mutant analyses necessary to integrate the roles of transporters and potential receptors with male gametophyte development.},
doi = {10.1104/pp.105.074708},
journal = {Plant Physiology},
number = ,
volume = 140,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}