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Title: Biodistribution of radioactive Cd 125mTe/ZnS nanoparticles targeted with antibody to murine lung endothelium.

Abstract

Radioactive cadmium telluride/zinc sulfide (Cd{sup 125m}Te/ZnS) nanoparticles were targeted to mouse lung with antibody to mouse lung endothelium and quantified using radiological histology in order to test the in vivo targeting efficacy of a nanoparticle-antibody (NP-mAb) system. The nanoparticles were linked to either a monoclonal antibody to mouse lung thrombomodulin (mAb 201B) or a control antibody (mAb 33), and injected into groups of 6-week-old Balb/C female mice. Animals were sacrificed at 1, 4, 24, 72 and 144 h post-injection, and biodistribution in major organs was determined. Full body microSPECT/CT imaging was performed on a pair of mice (experimental and control) providing visual confirmation of the biodistribution. The Cd{sup 125m}Te/ZnS NPs conjugated to mAb 201B principally target the lungs while the nanoparticles coupled to mAb 33 accumulate in the liver and spleen. These data provide, for the first time, a quantitative measurement of the in vivo targeting efficacy of an inorganic nanoparticle-mAb system.

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); High Temperature Materials Laboratory
Sponsoring Org.:
USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
931622
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Nanotechnology; Journal Volume: 18; Journal Issue: 17
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 77 NANOSCIENCE AND NANOTECHNOLOGY; CADMIUM 125; ENDOTHELIUM; IN VIVO; LUNGS; MICE; LIVER; SPLEEN; ZINC SULFIDES; CADMIUM TELLURIDES; NANOSTRUCTURES; ANTIBODIES; RADIONUCLIDE KINETICS; BIOLOGICAL LOCALIZATION

Citation Formats

Woodward, Jonathan, Kennel, Steve J, Mirzadeh, Saed, Dai, Sheng, and Rondinone, Adam Justin. Biodistribution of radioactive Cd125mTe/ZnS nanoparticles targeted with antibody to murine lung endothelium.. United States: N. p., 2007. Web. doi:10.1088/0957-4484/18/17/175103.
Woodward, Jonathan, Kennel, Steve J, Mirzadeh, Saed, Dai, Sheng, & Rondinone, Adam Justin. Biodistribution of radioactive Cd125mTe/ZnS nanoparticles targeted with antibody to murine lung endothelium.. United States. doi:10.1088/0957-4484/18/17/175103.
Woodward, Jonathan, Kennel, Steve J, Mirzadeh, Saed, Dai, Sheng, and Rondinone, Adam Justin. Mon . "Biodistribution of radioactive Cd125mTe/ZnS nanoparticles targeted with antibody to murine lung endothelium.". United States. doi:10.1088/0957-4484/18/17/175103.
@article{osti_931622,
title = {Biodistribution of radioactive Cd125mTe/ZnS nanoparticles targeted with antibody to murine lung endothelium.},
author = {Woodward, Jonathan and Kennel, Steve J and Mirzadeh, Saed and Dai, Sheng and Rondinone, Adam Justin},
abstractNote = {Radioactive cadmium telluride/zinc sulfide (Cd{sup 125m}Te/ZnS) nanoparticles were targeted to mouse lung with antibody to mouse lung endothelium and quantified using radiological histology in order to test the in vivo targeting efficacy of a nanoparticle-antibody (NP-mAb) system. The nanoparticles were linked to either a monoclonal antibody to mouse lung thrombomodulin (mAb 201B) or a control antibody (mAb 33), and injected into groups of 6-week-old Balb/C female mice. Animals were sacrificed at 1, 4, 24, 72 and 144 h post-injection, and biodistribution in major organs was determined. Full body microSPECT/CT imaging was performed on a pair of mice (experimental and control) providing visual confirmation of the biodistribution. The Cd{sup 125m}Te/ZnS NPs conjugated to mAb 201B principally target the lungs while the nanoparticles coupled to mAb 33 accumulate in the liver and spleen. These data provide, for the first time, a quantitative measurement of the in vivo targeting efficacy of an inorganic nanoparticle-mAb system.},
doi = {10.1088/0957-4484/18/17/175103},
journal = {Nanotechnology},
number = 17,
volume = 18,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}