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Title: Biodistribution of radioactive Cd 125mTe/ZnS nanoparticles targeted with antibody to murine lung endothelium.

Abstract

Radioactive cadmium telluride/zinc sulfide (Cd{sup 125m}Te/ZnS) nanoparticles were targeted to mouse lung with antibody to mouse lung endothelium and quantified using radiological histology in order to test the in vivo targeting efficacy of a nanoparticle-antibody (NP-mAb) system. The nanoparticles were linked to either a monoclonal antibody to mouse lung thrombomodulin (mAb 201B) or a control antibody (mAb 33), and injected into groups of 6-week-old Balb/C female mice. Animals were sacrificed at 1, 4, 24, 72 and 144 h post-injection, and biodistribution in major organs was determined. Full body microSPECT/CT imaging was performed on a pair of mice (experimental and control) providing visual confirmation of the biodistribution. The Cd{sup 125m}Te/ZnS NPs conjugated to mAb 201B principally target the lungs while the nanoparticles coupled to mAb 33 accumulate in the liver and spleen. These data provide, for the first time, a quantitative measurement of the in vivo targeting efficacy of an inorganic nanoparticle-mAb system.

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); High Temperature Materials Laboratory
Sponsoring Org.:
USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
931622
DOE Contract Number:
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Nanotechnology; Journal Volume: 18; Journal Issue: 17
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 77 NANOSCIENCE AND NANOTECHNOLOGY; CADMIUM 125; ENDOTHELIUM; IN VIVO; LUNGS; MICE; LIVER; SPLEEN; ZINC SULFIDES; CADMIUM TELLURIDES; NANOSTRUCTURES; ANTIBODIES; RADIONUCLIDE KINETICS; BIOLOGICAL LOCALIZATION

Citation Formats

Woodward, Jonathan, Kennel, Steve J, Mirzadeh, Saed, Dai, Sheng, and Rondinone, Adam Justin. Biodistribution of radioactive Cd125mTe/ZnS nanoparticles targeted with antibody to murine lung endothelium.. United States: N. p., 2007. Web. doi:10.1088/0957-4484/18/17/175103.
Woodward, Jonathan, Kennel, Steve J, Mirzadeh, Saed, Dai, Sheng, & Rondinone, Adam Justin. Biodistribution of radioactive Cd125mTe/ZnS nanoparticles targeted with antibody to murine lung endothelium.. United States. doi:10.1088/0957-4484/18/17/175103.
Woodward, Jonathan, Kennel, Steve J, Mirzadeh, Saed, Dai, Sheng, and Rondinone, Adam Justin. Mon . "Biodistribution of radioactive Cd125mTe/ZnS nanoparticles targeted with antibody to murine lung endothelium.". United States. doi:10.1088/0957-4484/18/17/175103.
@article{osti_931622,
title = {Biodistribution of radioactive Cd125mTe/ZnS nanoparticles targeted with antibody to murine lung endothelium.},
author = {Woodward, Jonathan and Kennel, Steve J and Mirzadeh, Saed and Dai, Sheng and Rondinone, Adam Justin},
abstractNote = {Radioactive cadmium telluride/zinc sulfide (Cd{sup 125m}Te/ZnS) nanoparticles were targeted to mouse lung with antibody to mouse lung endothelium and quantified using radiological histology in order to test the in vivo targeting efficacy of a nanoparticle-antibody (NP-mAb) system. The nanoparticles were linked to either a monoclonal antibody to mouse lung thrombomodulin (mAb 201B) or a control antibody (mAb 33), and injected into groups of 6-week-old Balb/C female mice. Animals were sacrificed at 1, 4, 24, 72 and 144 h post-injection, and biodistribution in major organs was determined. Full body microSPECT/CT imaging was performed on a pair of mice (experimental and control) providing visual confirmation of the biodistribution. The Cd{sup 125m}Te/ZnS NPs conjugated to mAb 201B principally target the lungs while the nanoparticles coupled to mAb 33 accumulate in the liver and spleen. These data provide, for the first time, a quantitative measurement of the in vivo targeting efficacy of an inorganic nanoparticle-mAb system.},
doi = {10.1088/0957-4484/18/17/175103},
journal = {Nanotechnology},
number = 17,
volume = 18,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}
  • The relative abundances of the fragment ions that result respectively from the nuclear decays of CH 3I 125 and C 2H 5I 125 (I 125 undergoes electron capture to give Te 125) were measured with a specially designed mass spectrometer. The data from both studies are similar and give evidence of the highly destructive nature of the decay: only about 1% of the Te--hydrocarbon ions appear to remain intact in spite of the fact that the ions would be expected to be collected with high efficiency; in both studies a large number of tellurium ions are found with charges asmore » high as 18 and with an average charge of about 9; also in both studies, the singly, doubly, and triply charged carbon ions are found in greater abundance than the hydrocarbon ions. The cause of this extensive ionization and fragmentation is ascribed primarily to a series of Auger processes that occur subsequently to the formation of inner orbital vacancies in Te 125 as the result of electron capture and internal conversion in the decay of I 125.« less
  • Nuclear magnetic resonance measurements on oriented nuclei have been performed for /sup 125/Te/sup m/, /sup 127/Te/sup m/, and /sup 129/Te/sup m/ using low temperature nuclear orientation and conversion electron detection. The magnetic moments of the 11/2/sup -/ isomeric states have been extracted as ..mu..=-0.985(6) for /sup 125/Te/sup m/, ..mu..=-1.041(6) for /sup 127/Te/sup m/, and ..mu..=-1.091(7) for /sup 129/Te/sup m/.
  • The complexes (M(o-C{sub 6}H{sub 4}(TeMe){sub 2})X{sub 2}) (M = Pd, Pt; X = Cl, Br, I) and (N{sup n}Bu{sub 4})(Ir(o-C{sub 6}H{sub 4}(TeMe){sub 2})X{sub 4}) (X = Cl, Br) have been prepared, and characterized by analysis and uv-visible, ir, and multinuclear ({sup 1}H, {sup 195}Pt({sup 1}H), {sup 125}Te({sup 1}H))NMR spectroscopy. Two isomers, meso and dl invertomers, are present in each complex. The coordination shifts in the {sup 125}Te NMR spectra of the Pd and Pt complexes are compared with those reported for (M(PhTe(CH{sub 2}){sub 3}TePh)X{sub 2}) and for the analogous cis-(M(TeMePh){sub 2}x{sub 2}). It is found that, compared with the monodentatemore » complexes, the chelate complexes having five-membered rings exhibit large high frequency coordination shifts and those having six-membered rings have small low frequency shifts. Data on complexes of o-C{sub 6}H{sub 4}(TePh){sub 2} and MeTe(CH{sub 2}){sub 3}TeMe compared with data for TePh{sub 2} and TeMe{sub 2} complexes show similar effects, which are rationalized in terms of a characteristic chelate ring contribution, defined as {Delta}{sub R}, to the shifts of the bidentate telluroethers. Similar phenomena are known for {sup 31}P and {sup 77}Se shifts in analogous complexes, but this is the first demonstration of the effect in {sup 125}Te NMR. 21 refs., 3 figs., 4 tabs.« less