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Title: Genome Sequence and Analysis of the Soil Cellulolytic Actinomycete Thermobifida fusca YX

Abstract

Thermobifida fusca is a moderately thermophilic soil bacterium that belongs to Actinobacteria. It is a major degrader of plant cell walls and has been used as a model organism for the study of secreted, thermostable cellulases. The complete genome sequence showed that T. fusca has a single circular chromosome of 3,642,249 bp predicted to encode 3,117 proteins and 65 RNA species with a coding density of 85%. Genome analysis revealed the existence of 29 putative glycoside hydrolases in addition to the previously identified cellulases and xylanases. The glycosyl hydrolases include enzymes predicted to exhibit mainly dextran/starch- and xylan-degrading functions. T. fusca possesses two protein secretion systems: the sec general secretion system and the twin-arginine translocation system. Several of the secreted cellulases have sequence signatures indicating their secretion may be mediated by the twin-arginine translocation system. T. fusca has extensive transport systems for import of carbohydrates coupled to transcriptional regulators controlling the expression of the transporters and glycosylhydrolases. In addition to providing an overview of the physiology of a soil actinomycete, this study presents insights on the transcriptional regulation and secretion of cellulases which may facilitate the industrial exploitation of these systems.

Authors:
 [1];  [1];  [1];  [1];  [2];  [1];  [1];  [1];  [1];  [1];  [1];  [3];  [1]
  1. U.S. Department of Energy, Joint Genome Institute
  2. ORNL
  3. Cornell University
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
931550
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Bacteriology; Journal Volume: 189; Journal Issue: 6
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; GLYCOSYL HYDROLASES; PLANT CELLS; SECRETION; SOILS; CELLULASE

Citation Formats

Lykidis, A, Mavromatis, K, Ivanova, N, Anderson, Iain, Land, Miriam L, DiBartolo, Genevieve, Martinez, Michele, Lapidus, Alla L., Lucas, Susan, Copeland, A, Richardson, P M, Wilson, David B, and Kyrpides, Nikos C. Genome Sequence and Analysis of the Soil Cellulolytic Actinomycete Thermobifida fusca YX. United States: N. p., 2007. Web. doi:10.1128/JB.01899-06.
Lykidis, A, Mavromatis, K, Ivanova, N, Anderson, Iain, Land, Miriam L, DiBartolo, Genevieve, Martinez, Michele, Lapidus, Alla L., Lucas, Susan, Copeland, A, Richardson, P M, Wilson, David B, & Kyrpides, Nikos C. Genome Sequence and Analysis of the Soil Cellulolytic Actinomycete Thermobifida fusca YX. United States. doi:10.1128/JB.01899-06.
Lykidis, A, Mavromatis, K, Ivanova, N, Anderson, Iain, Land, Miriam L, DiBartolo, Genevieve, Martinez, Michele, Lapidus, Alla L., Lucas, Susan, Copeland, A, Richardson, P M, Wilson, David B, and Kyrpides, Nikos C. Mon . "Genome Sequence and Analysis of the Soil Cellulolytic Actinomycete Thermobifida fusca YX". United States. doi:10.1128/JB.01899-06.
@article{osti_931550,
title = {Genome Sequence and Analysis of the Soil Cellulolytic Actinomycete Thermobifida fusca YX},
author = {Lykidis, A and Mavromatis, K and Ivanova, N and Anderson, Iain and Land, Miriam L and DiBartolo, Genevieve and Martinez, Michele and Lapidus, Alla L. and Lucas, Susan and Copeland, A and Richardson, P M and Wilson, David B and Kyrpides, Nikos C},
abstractNote = {Thermobifida fusca is a moderately thermophilic soil bacterium that belongs to Actinobacteria. It is a major degrader of plant cell walls and has been used as a model organism for the study of secreted, thermostable cellulases. The complete genome sequence showed that T. fusca has a single circular chromosome of 3,642,249 bp predicted to encode 3,117 proteins and 65 RNA species with a coding density of 85%. Genome analysis revealed the existence of 29 putative glycoside hydrolases in addition to the previously identified cellulases and xylanases. The glycosyl hydrolases include enzymes predicted to exhibit mainly dextran/starch- and xylan-degrading functions. T. fusca possesses two protein secretion systems: the sec general secretion system and the twin-arginine translocation system. Several of the secreted cellulases have sequence signatures indicating their secretion may be mediated by the twin-arginine translocation system. T. fusca has extensive transport systems for import of carbohydrates coupled to transcriptional regulators controlling the expression of the transporters and glycosylhydrolases. In addition to providing an overview of the physiology of a soil actinomycete, this study presents insights on the transcriptional regulation and secretion of cellulases which may facilitate the industrial exploitation of these systems.},
doi = {10.1128/JB.01899-06},
journal = {Journal of Bacteriology},
number = 6,
volume = 189,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}