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Title: Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Acute Chromate Challenge

Abstract

Proteome alterations in the metal-reducing bacterium Shewanella oneidensis MR-1 in response to different acute dose challenges (0.3, 0.5, or 1 mM) of the toxic metal chromate [Cr(VI)] were characterized with multidimensional HPLC-MS/MS on a linear trapping quadrupole MS. A total of 2,406 functionally diverse proteins were identified, with a subset demonstrating dosage-dependent up- and down-regulated expression, such as proteins involved in detoxification and iron binding and transport.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2]
  1. ORNL
  2. {Bob} L [ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
931468
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Proteome Research; Journal Volume: 6; Journal Issue: 5
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHROMATES; DETOXIFICATION; IRON; PROTEINS; QUADRUPOLES; TRANSPORT; TRAPPING; Mass Spectrometry; multi-dimensional liquid chromatography; shotgun proteomics; Shewanella oneidensis; Linear Trapping Quadrupole; chromate stress

Citation Formats

Thompson, Melissa R, Verberkmoes, Nathan C, Chourey, Karuna, Shah, Manesh B, Thompson, Dorothea K, and Hettich, Robert. Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Acute Chromate Challenge. United States: N. p., 2007. Web. doi:10.1021/pr060502x.
Thompson, Melissa R, Verberkmoes, Nathan C, Chourey, Karuna, Shah, Manesh B, Thompson, Dorothea K, & Hettich, Robert. Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Acute Chromate Challenge. United States. doi:10.1021/pr060502x.
Thompson, Melissa R, Verberkmoes, Nathan C, Chourey, Karuna, Shah, Manesh B, Thompson, Dorothea K, and Hettich, Robert. Mon . "Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Acute Chromate Challenge". United States. doi:10.1021/pr060502x.
@article{osti_931468,
title = {Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Acute Chromate Challenge},
author = {Thompson, Melissa R and Verberkmoes, Nathan C and Chourey, Karuna and Shah, Manesh B and Thompson, Dorothea K and Hettich, Robert},
abstractNote = {Proteome alterations in the metal-reducing bacterium Shewanella oneidensis MR-1 in response to different acute dose challenges (0.3, 0.5, or 1 mM) of the toxic metal chromate [Cr(VI)] were characterized with multidimensional HPLC-MS/MS on a linear trapping quadrupole MS. A total of 2,406 functionally diverse proteins were identified, with a subset demonstrating dosage-dependent up- and down-regulated expression, such as proteins involved in detoxification and iron binding and transport.},
doi = {10.1021/pr060502x},
journal = {Journal of Proteome Research},
number = 5,
volume = 6,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}