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Title: Salt Tolerance of Desorption Electrospray Ionization (DESI)

Abstract

Suppression of ion intensity in the presence of high salt matrices is common in most mass spectrometry ionization techniques. Desorption electrospray ionization (DESI) is an ionization method that exhibits salt tolerance, and this is investigated. DESI analysis was performed on three different drug mixtures in the presence of 0, 0.2, 2, 5, 10, and 20% NaCl:KCl weight by volume from seven different surfaces. At physiological concentrations individual drugs in each mixture were observed with each surface. Collision-induced dissociation (CID) was used to provide additional confirmation for select compounds. Multiple stage experiments, to MS5, were performed for select compounds. Even in the absence of added salt, the benzodiazepine containing mixture yielded sodium and potassium adducts of carbamazepine which masked the ions of interest. These adducts were eliminated by adding 0.1% 7M ammonium acetate to the standard methanol:water (1:1) spray solvent. Comparison of the salt tolerance of DESI with that of electrospray ionization (ESI) demonstrated much better signal/noise characteristics for DESI in this study. The salt tolerance of DESI was also studied by performing limit of detection and dynamic range experiments. Even at a salt concentration significantly above physiological concentrations, select surfaces were effective in providing spectra that allowed the ready identificationmore » of the compounds of interest. The already high salt tolerance of DESI can be optimized further by appropriate choices of surface and spray solution.« less

Authors:
 [1];  [1];  [1];  [2]
  1. Purdue University
  2. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
930939
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of the American Society for Mass Spectrometry; Journal Volume: 18; Journal Issue: 2
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; MASS SPECTROSCOPY; SPRAYS; IONIZATION; SALTS; TOLERANCE; DRUGS; MIXTURES; PERFORMANCE; barbiturates; benzodiazepines; cocaine; desorption electrospray ionization; forensics; ion suppression; matrix effects; oligonucleotide

Citation Formats

Jackson, Ayanna U., Talaty, Nari, Cooks, R G, and Van Berkel, Gary J. Salt Tolerance of Desorption Electrospray Ionization (DESI). United States: N. p., 2007. Web. doi:10.1016/j.jasms.2007.09.018.
Jackson, Ayanna U., Talaty, Nari, Cooks, R G, & Van Berkel, Gary J. Salt Tolerance of Desorption Electrospray Ionization (DESI). United States. doi:10.1016/j.jasms.2007.09.018.
Jackson, Ayanna U., Talaty, Nari, Cooks, R G, and Van Berkel, Gary J. Mon . "Salt Tolerance of Desorption Electrospray Ionization (DESI)". United States. doi:10.1016/j.jasms.2007.09.018.
@article{osti_930939,
title = {Salt Tolerance of Desorption Electrospray Ionization (DESI)},
author = {Jackson, Ayanna U. and Talaty, Nari and Cooks, R G and Van Berkel, Gary J},
abstractNote = {Suppression of ion intensity in the presence of high salt matrices is common in most mass spectrometry ionization techniques. Desorption electrospray ionization (DESI) is an ionization method that exhibits salt tolerance, and this is investigated. DESI analysis was performed on three different drug mixtures in the presence of 0, 0.2, 2, 5, 10, and 20% NaCl:KCl weight by volume from seven different surfaces. At physiological concentrations individual drugs in each mixture were observed with each surface. Collision-induced dissociation (CID) was used to provide additional confirmation for select compounds. Multiple stage experiments, to MS5, were performed for select compounds. Even in the absence of added salt, the benzodiazepine containing mixture yielded sodium and potassium adducts of carbamazepine which masked the ions of interest. These adducts were eliminated by adding 0.1% 7M ammonium acetate to the standard methanol:water (1:1) spray solvent. Comparison of the salt tolerance of DESI with that of electrospray ionization (ESI) demonstrated much better signal/noise characteristics for DESI in this study. The salt tolerance of DESI was also studied by performing limit of detection and dynamic range experiments. Even at a salt concentration significantly above physiological concentrations, select surfaces were effective in providing spectra that allowed the ready identification of the compounds of interest. The already high salt tolerance of DESI can be optimized further by appropriate choices of surface and spray solution.},
doi = {10.1016/j.jasms.2007.09.018},
journal = {Journal of the American Society for Mass Spectrometry},
number = 2,
volume = 18,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}