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Title: Global Molecular and Morphological Effects of 24-Hour Chromium(VI)Exposure on Shewanella oneidensis MR-1

Abstract

The biological impact of 24-h ("chronic") chromium(VI) [Cr(VI) or chromate] exposure on ShewanellaoneidensisMR-1 was assessed by analyzing cellular morphology as well as genome-wide differential gene and protein expression profiles. Cells challenged aerobically with an initial chromate concentration of 0.3 mM in complex growth medium were compared to untreated control cells grown in the absence of chromate. At the 24-h time point at which cells were harvested for transcriptome and proteome analyses, no residual Cr(VI) was detected in the culture supernatant, thus suggesting the complete uptake and/or reduction of this metal by cells. In contrast to the untreated control cells, Cr(VI)-exposed cells formed apparently aseptate, nonmotile filaments that tended to aggregate. Transcriptome profiling and mass spectrometry-based proteomic charac terization revealed that the principal molecular response to 24-h Cr(VI) exposure was the induction of prophage-related genes and their encoded products as well as a number of functionally undefined hypothetical genes that were located within the integrated phage regions of the MR-1 genome. In addition, genes with annotated functions in DNA metabolism, cell division, biosynthesis and degradation of the murein (pepti doglycan) sacculus, membrane response, and general environmental stress protection were upregulated, while genes encoding chemotaxis, motility, and transport/binding proteins were largely repressedmore » under conditions of 24-h chromate treatment.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [1]
  1. ORNL
  2. {Bob} L [ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
930903
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied and Environmental Microbiology; Journal Volume: 72; Journal Issue: 9
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BIOSYNTHESIS; CELL DIVISION; CHROMATES; DNA; GENES; INDUCTION; MEMBRANES; METABOLISM; MORPHOLOGY; PROTEINS

Citation Formats

Chourey, Karuna, Thompson, Melissa R, Morrell-Falvey, Jennifer L, Verberkmoes, Nathan C, Brown, Steven D, Shah, Manesh B, Zhou, Jizhong, Doktycz, Mitchel John, Hettich, Robert, and Thompson, Dorothea K. Global Molecular and Morphological Effects of 24-Hour Chromium(VI)Exposure on Shewanella oneidensis MR-1. United States: N. p., 2006. Web. doi:10.1128/AEM.00813-06.
Chourey, Karuna, Thompson, Melissa R, Morrell-Falvey, Jennifer L, Verberkmoes, Nathan C, Brown, Steven D, Shah, Manesh B, Zhou, Jizhong, Doktycz, Mitchel John, Hettich, Robert, & Thompson, Dorothea K. Global Molecular and Morphological Effects of 24-Hour Chromium(VI)Exposure on Shewanella oneidensis MR-1. United States. doi:10.1128/AEM.00813-06.
Chourey, Karuna, Thompson, Melissa R, Morrell-Falvey, Jennifer L, Verberkmoes, Nathan C, Brown, Steven D, Shah, Manesh B, Zhou, Jizhong, Doktycz, Mitchel John, Hettich, Robert, and Thompson, Dorothea K. Sun . "Global Molecular and Morphological Effects of 24-Hour Chromium(VI)Exposure on Shewanella oneidensis MR-1". United States. doi:10.1128/AEM.00813-06.
@article{osti_930903,
title = {Global Molecular and Morphological Effects of 24-Hour Chromium(VI)Exposure on Shewanella oneidensis MR-1},
author = {Chourey, Karuna and Thompson, Melissa R and Morrell-Falvey, Jennifer L and Verberkmoes, Nathan C and Brown, Steven D and Shah, Manesh B and Zhou, Jizhong and Doktycz, Mitchel John and Hettich, Robert and Thompson, Dorothea K},
abstractNote = {The biological impact of 24-h ("chronic") chromium(VI) [Cr(VI) or chromate] exposure on ShewanellaoneidensisMR-1 was assessed by analyzing cellular morphology as well as genome-wide differential gene and protein expression profiles. Cells challenged aerobically with an initial chromate concentration of 0.3 mM in complex growth medium were compared to untreated control cells grown in the absence of chromate. At the 24-h time point at which cells were harvested for transcriptome and proteome analyses, no residual Cr(VI) was detected in the culture supernatant, thus suggesting the complete uptake and/or reduction of this metal by cells. In contrast to the untreated control cells, Cr(VI)-exposed cells formed apparently aseptate, nonmotile filaments that tended to aggregate. Transcriptome profiling and mass spectrometry-based proteomic charac terization revealed that the principal molecular response to 24-h Cr(VI) exposure was the induction of prophage-related genes and their encoded products as well as a number of functionally undefined hypothetical genes that were located within the integrated phage regions of the MR-1 genome. In addition, genes with annotated functions in DNA metabolism, cell division, biosynthesis and degradation of the murein (pepti doglycan) sacculus, membrane response, and general environmental stress protection were upregulated, while genes encoding chemotaxis, motility, and transport/binding proteins were largely repressed under conditions of 24-h chromate treatment.},
doi = {10.1128/AEM.00813-06},
journal = {Applied and Environmental Microbiology},
number = 9,
volume = 72,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}