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Title: Toward Understanding Phosphoseryl-tRNA Cys Formation: The Crystal Structure of Methanococcus maripaludis Phosphoseryl-tRNA Synthetase

Abstract

A number of archaeal organisms generate Cys-tRNA{sup Cys} in a two-step pathway, first charging phosphoserine (Sep) onto tRNA{sup Cys} and subsequently converting it to Cys-tRNA{sup Cys}. We have determined, at 3.2-{angstrom} resolution, the structure of the Methanococcus maripaludis phosphoseryl-tRNA synthetase (SepRS), which catalyzes the first step of this pathway. The structure shows that SepRS is a class II, {alpha}{sub 4} synthetase whose quaternary structure arrangement of subunits closely resembles that of the heterotetrameric ({alpha}{beta}){sub 2} phenylalanyl-tRNA synthetase (PheRS). Homology modeling of a tRNA complex indicates that, in contrast to PheRS, a single monomer in the SepRS tetramer may recognize both the acceptor terminus and anticodon of a tRNA substrate. Using a complex with tungstate as a marker for the position of the phosphate moiety of Sep, we suggest that SepRS and PheRS bind their respective amino acid substrates in dissimilar orientations by using different residues.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
930394
Report Number(s):
BNL-81116-2008-JA
Journal ID: ISSN 0027-8424; PNASA6; TRN: US200904%%674
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proceedings of the National Academy of Sciences of the USA; Journal Volume: 104; Journal Issue: 8
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; AMINO ACIDS; CRYSTAL STRUCTURE; CYSTEINE; LIGASES; MONOMERS; PHOSPHATES; RESIDUES; SIMULATION; SUBSTRATES; TRANSFER RNA; TUNGSTATES; national synchrotron light source

Citation Formats

Kamtekar ,S., Hohn, M., Park, h., Schnitzbauer, M., Sauerwald, A., Soll, D., and Steitz, T.. Toward Understanding Phosphoseryl-tRNA Cys Formation: The Crystal Structure of Methanococcus maripaludis Phosphoseryl-tRNA Synthetase. United States: N. p., 2007. Web.
Kamtekar ,S., Hohn, M., Park, h., Schnitzbauer, M., Sauerwald, A., Soll, D., & Steitz, T.. Toward Understanding Phosphoseryl-tRNA Cys Formation: The Crystal Structure of Methanococcus maripaludis Phosphoseryl-tRNA Synthetase. United States.
Kamtekar ,S., Hohn, M., Park, h., Schnitzbauer, M., Sauerwald, A., Soll, D., and Steitz, T.. Mon . "Toward Understanding Phosphoseryl-tRNA Cys Formation: The Crystal Structure of Methanococcus maripaludis Phosphoseryl-tRNA Synthetase". United States. doi:.
@article{osti_930394,
title = {Toward Understanding Phosphoseryl-tRNA Cys Formation: The Crystal Structure of Methanococcus maripaludis Phosphoseryl-tRNA Synthetase},
author = {Kamtekar ,S. and Hohn, M. and Park, h. and Schnitzbauer, M. and Sauerwald, A. and Soll, D. and Steitz, T.},
abstractNote = {A number of archaeal organisms generate Cys-tRNA{sup Cys} in a two-step pathway, first charging phosphoserine (Sep) onto tRNA{sup Cys} and subsequently converting it to Cys-tRNA{sup Cys}. We have determined, at 3.2-{angstrom} resolution, the structure of the Methanococcus maripaludis phosphoseryl-tRNA synthetase (SepRS), which catalyzes the first step of this pathway. The structure shows that SepRS is a class II, {alpha}{sub 4} synthetase whose quaternary structure arrangement of subunits closely resembles that of the heterotetrameric ({alpha}{beta}){sub 2} phenylalanyl-tRNA synthetase (PheRS). Homology modeling of a tRNA complex indicates that, in contrast to PheRS, a single monomer in the SepRS tetramer may recognize both the acceptor terminus and anticodon of a tRNA substrate. Using a complex with tungstate as a marker for the position of the phosphate moiety of Sep, we suggest that SepRS and PheRS bind their respective amino acid substrates in dissimilar orientations by using different residues.},
doi = {},
journal = {Proceedings of the National Academy of Sciences of the USA},
number = 8,
volume = 104,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}