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Title: Structural Analysis of the Plakin Domain of Bullous Pemphigiod Antigen1 (BPAG1) Suggests That Plakins are Members of the Spectrin Superfamily

Abstract

Bullous pemphigoid antigen 1 (BPAG1) is a member of the plakin family of proteins. The plakins are multi-domain proteins that have been shown to interact with microtubules, actin filaments and intermediate filaments, as well as proteins found in cellular junctions. These interactions are mediated through different domains on the plakins. The interactions between plakins and components of specialized cell junctions such as desmosomes and hemidesmosomes are mediated through the so-called plakin domain, which is a common feature of the plakins. We report the crystal structure of a stable fragment from BPAG1, residues 226-448, defined by limited proteolysis of the whole plakin domain. The structure, determined by single-wavelength anomalous diffraction phasing from a selenomethionine-substituted crystal at 3.0 {angstrom} resolution, reveals a tandem pair of triple helical bundles closely related to spectrin repeats. Based on this structure and analysis of sequence conservation, we propose that the architecture of plakin domains is defined by two pairs of spectrin repeats interrupted by a putative Src-Homology 3 (SH3) domain.

Authors:
; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
930287
Report Number(s):
BNL-80991-2008-JA
Journal ID: ISSN 0022-2836; JMOBAK; TRN: US200822%%1449
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Molecular Biology; Journal Volume: 366; Journal Issue: 1
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; ACTIN; ANTIGENS; ARCHITECTURE; CRYSTAL STRUCTURE; CRYSTALS; DIFFRACTION; FILAMENTS; INTERACTIONS; MICROTUBULES; PROTEINS; PROTEOLYSIS; RESIDUES; RESOLUTION; national synchrotron light source

Citation Formats

Jefferson,J., Ciatto, C., Shapiro, l., and Liem, R. Structural Analysis of the Plakin Domain of Bullous Pemphigiod Antigen1 (BPAG1) Suggests That Plakins are Members of the Spectrin Superfamily. United States: N. p., 2007. Web. doi:10.1016/j.jmb.2006.11.036.
Jefferson,J., Ciatto, C., Shapiro, l., & Liem, R. Structural Analysis of the Plakin Domain of Bullous Pemphigiod Antigen1 (BPAG1) Suggests That Plakins are Members of the Spectrin Superfamily. United States. doi:10.1016/j.jmb.2006.11.036.
Jefferson,J., Ciatto, C., Shapiro, l., and Liem, R. Mon . "Structural Analysis of the Plakin Domain of Bullous Pemphigiod Antigen1 (BPAG1) Suggests That Plakins are Members of the Spectrin Superfamily". United States. doi:10.1016/j.jmb.2006.11.036.
@article{osti_930287,
title = {Structural Analysis of the Plakin Domain of Bullous Pemphigiod Antigen1 (BPAG1) Suggests That Plakins are Members of the Spectrin Superfamily},
author = {Jefferson,J. and Ciatto, C. and Shapiro, l. and Liem, R.},
abstractNote = {Bullous pemphigoid antigen 1 (BPAG1) is a member of the plakin family of proteins. The plakins are multi-domain proteins that have been shown to interact with microtubules, actin filaments and intermediate filaments, as well as proteins found in cellular junctions. These interactions are mediated through different domains on the plakins. The interactions between plakins and components of specialized cell junctions such as desmosomes and hemidesmosomes are mediated through the so-called plakin domain, which is a common feature of the plakins. We report the crystal structure of a stable fragment from BPAG1, residues 226-448, defined by limited proteolysis of the whole plakin domain. The structure, determined by single-wavelength anomalous diffraction phasing from a selenomethionine-substituted crystal at 3.0 {angstrom} resolution, reveals a tandem pair of triple helical bundles closely related to spectrin repeats. Based on this structure and analysis of sequence conservation, we propose that the architecture of plakin domains is defined by two pairs of spectrin repeats interrupted by a putative Src-Homology 3 (SH3) domain.},
doi = {10.1016/j.jmb.2006.11.036},
journal = {Journal of Molecular Biology},
number = 1,
volume = 366,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}