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Title: Chemical Imaging of Biological Tissue with Synchrotron Infrared Light

Abstract

Fourier transform infrared micro-spectroscopy (FTIRM) and imaging (FTIRI) have become valuable techniques for examining the chemical makeup of biological materials by probing their vibrational motions on a microscopic scale. Synchrotron infrared (S-IR) light is an ideal source for FTIRM and FTIRI due to the combination of its high brightness (i.e., flux density), also called brilliance, and broadband nature. Through a 10-{mu}m pinhole, the brightness of a synchrotron source is 100-1000 times higher than a conventional thermal (globar) source. Accordingly, the improvement in spatial resolution and in spectral quality to the diffraction limit has led to a plethora of applications that is just being realized. In this review, we describe the development of synchrotron-based FTIRM, illustrate its advantages in many applications to biological systems, and propose some potential future directions for the technique.

Authors:
;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
930119
Report Number(s):
BNL-80764-2008-JA
Journal ID: ISSN 0006-3002; BBACAQ; TRN: US0806709
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochimica et Biophysica Acta; Journal Volume: 1758; Journal Issue: 7
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; BIOLOGICAL MATERIALS; BRIGHTNESS; DENSITY; DIFFRACTION; SPATIAL RESOLUTION; SYNCHROTRONS; USES; VISIBLE RADIATION; national synchrotron light source

Citation Formats

Miller,L., and Dumas, P.. Chemical Imaging of Biological Tissue with Synchrotron Infrared Light. United States: N. p., 2006. Web. doi:10.1016/j.bbamem.2006.04.010.
Miller,L., & Dumas, P.. Chemical Imaging of Biological Tissue with Synchrotron Infrared Light. United States. doi:10.1016/j.bbamem.2006.04.010.
Miller,L., and Dumas, P.. Sun . "Chemical Imaging of Biological Tissue with Synchrotron Infrared Light". United States. doi:10.1016/j.bbamem.2006.04.010.
@article{osti_930119,
title = {Chemical Imaging of Biological Tissue with Synchrotron Infrared Light},
author = {Miller,L. and Dumas, P.},
abstractNote = {Fourier transform infrared micro-spectroscopy (FTIRM) and imaging (FTIRI) have become valuable techniques for examining the chemical makeup of biological materials by probing their vibrational motions on a microscopic scale. Synchrotron infrared (S-IR) light is an ideal source for FTIRM and FTIRI due to the combination of its high brightness (i.e., flux density), also called brilliance, and broadband nature. Through a 10-{mu}m pinhole, the brightness of a synchrotron source is 100-1000 times higher than a conventional thermal (globar) source. Accordingly, the improvement in spatial resolution and in spectral quality to the diffraction limit has led to a plethora of applications that is just being realized. In this review, we describe the development of synchrotron-based FTIRM, illustrate its advantages in many applications to biological systems, and propose some potential future directions for the technique.},
doi = {10.1016/j.bbamem.2006.04.010},
journal = {Biochimica et Biophysica Acta},
number = 7,
volume = 1758,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}