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Title: Accretion of Bone Quantity and Quality in the Developing Mouse Skeleton

Abstract

To meet the mechanical challenges during early development, the skeleton requires the rapid accretion of bone quality and bone quantity. Here, we describe early bone development in the mouse skeleton and test the hypothesis that specific compositional properties determine the stiffness of the tissue. Tibias of female BALB mice were harvested at eight time-points (n = 4 each) distributed between 1 and 40 days of age and subjected to morphometric ({mu}CT), chemical (Fourier transform infrared microscpectroscopy), and mechanical (nanoindentation) analyses. Tibias of 450-day-old mice served as fully mineralized control specimens. In this work, we found that bone mineral formation proceeded very rapidly in mice by 1 day of age, where the degree of mineralization, the tissue mineral density, and the mineral crystallinity reached 36%, 51%, and 87% of the adult values, respectively. However, even though significant mineralization had occurred, the elastic modulus of 1-day-old bone was only 14% of its adult value, indicating that the intrinsic stiffening of the bone lags considerably behind the initial mineral formation.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
929967
Report Number(s):
BNL-80571-2008-JA
Journal ID: ISSN 0884-0431; JBMREJ; TRN: US200822%%947
DOE Contract Number:  
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Bone and Mineral Research; Journal Volume: 22; Journal Issue: 7
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DENSITY; FEMALES; FLEXIBILITY; HYPOTHESIS; MICE; MINERALIZATION; MINERALS; SKELETON; national synchrotron light source

Citation Formats

Miller,L., Little, W., Schirmer, A., Sheik, F., Busa, B., and Judex, S.. Accretion of Bone Quantity and Quality in the Developing Mouse Skeleton. United States: N. p., 2007. Web. doi:10.1359/jbmr.070402.
Miller,L., Little, W., Schirmer, A., Sheik, F., Busa, B., & Judex, S.. Accretion of Bone Quantity and Quality in the Developing Mouse Skeleton. United States. doi:10.1359/jbmr.070402.
Miller,L., Little, W., Schirmer, A., Sheik, F., Busa, B., and Judex, S.. Mon . "Accretion of Bone Quantity and Quality in the Developing Mouse Skeleton". United States. doi:10.1359/jbmr.070402.
@article{osti_929967,
title = {Accretion of Bone Quantity and Quality in the Developing Mouse Skeleton},
author = {Miller,L. and Little, W. and Schirmer, A. and Sheik, F. and Busa, B. and Judex, S.},
abstractNote = {To meet the mechanical challenges during early development, the skeleton requires the rapid accretion of bone quality and bone quantity. Here, we describe early bone development in the mouse skeleton and test the hypothesis that specific compositional properties determine the stiffness of the tissue. Tibias of female BALB mice were harvested at eight time-points (n = 4 each) distributed between 1 and 40 days of age and subjected to morphometric ({mu}CT), chemical (Fourier transform infrared microscpectroscopy), and mechanical (nanoindentation) analyses. Tibias of 450-day-old mice served as fully mineralized control specimens. In this work, we found that bone mineral formation proceeded very rapidly in mice by 1 day of age, where the degree of mineralization, the tissue mineral density, and the mineral crystallinity reached 36%, 51%, and 87% of the adult values, respectively. However, even though significant mineralization had occurred, the elastic modulus of 1-day-old bone was only 14% of its adult value, indicating that the intrinsic stiffening of the bone lags considerably behind the initial mineral formation.},
doi = {10.1359/jbmr.070402},
journal = {Journal of Bone and Mineral Research},
number = 7,
volume = 22,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}