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Title: Alteration of Iron-rich Lacustrine Sediments by Dissimilatory Iron-reducing Bacteria

Abstract

The reactivity of trace elements in lake sediments towards microbial metal reduction was evaluated using spectroscopy, chemical extractions and incubations in a minimal media with the DIR bacterium Shewanella putrefaciens 200R. Micro-XRF measurements demonstrated the association of Cr, and Ni with Mn-rich phases. The onset of anaerobic conditions resulted in the rapid release of trace metals (Cr, Ni, Co) from the sediments with the progressive dissolution of a reactive Mn component. This fraction was approximately equivalent to that liberated by chemical extractions designed to operationally select for Mn phases. These results suggest that studies aiming to assess metal dissolution in anaerobic soils and sediments should attempt to discriminate between metals associated with Mn and Fe (hydr)oxides, the former being more reactive and likely dissolved to a greater extent.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
929915
Report Number(s):
BNL-80503-2008-JA
Journal ID: ISSN 0149-0451; GEJODG; TRN: US200822%%1089
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Geomicrobiology Journal; Journal Volume: 24
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; ANAEROBIC CONDITIONS; BACTERIA; DISSOLUTION; ELEMENTS; LAKES; METALS; REACTIVITY; REDUCTION; SEDIMENTS; SOILS; SPECTROSCOPY; TRACE AMOUNTS; national synchrotron light source

Citation Formats

Crowe,S., O'Niell, A., Kulezycki, E., Weisener, C., Roberts, J., and Fowle, D. Alteration of Iron-rich Lacustrine Sediments by Dissimilatory Iron-reducing Bacteria. United States: N. p., 2007. Web. doi:10.1080/01490450701457329.
Crowe,S., O'Niell, A., Kulezycki, E., Weisener, C., Roberts, J., & Fowle, D. Alteration of Iron-rich Lacustrine Sediments by Dissimilatory Iron-reducing Bacteria. United States. doi:10.1080/01490450701457329.
Crowe,S., O'Niell, A., Kulezycki, E., Weisener, C., Roberts, J., and Fowle, D. Mon . "Alteration of Iron-rich Lacustrine Sediments by Dissimilatory Iron-reducing Bacteria". United States. doi:10.1080/01490450701457329.
@article{osti_929915,
title = {Alteration of Iron-rich Lacustrine Sediments by Dissimilatory Iron-reducing Bacteria},
author = {Crowe,S. and O'Niell, A. and Kulezycki, E. and Weisener, C. and Roberts, J. and Fowle, D.},
abstractNote = {The reactivity of trace elements in lake sediments towards microbial metal reduction was evaluated using spectroscopy, chemical extractions and incubations in a minimal media with the DIR bacterium Shewanella putrefaciens 200R. Micro-XRF measurements demonstrated the association of Cr, and Ni with Mn-rich phases. The onset of anaerobic conditions resulted in the rapid release of trace metals (Cr, Ni, Co) from the sediments with the progressive dissolution of a reactive Mn component. This fraction was approximately equivalent to that liberated by chemical extractions designed to operationally select for Mn phases. These results suggest that studies aiming to assess metal dissolution in anaerobic soils and sediments should attempt to discriminate between metals associated with Mn and Fe (hydr)oxides, the former being more reactive and likely dissolved to a greater extent.},
doi = {10.1080/01490450701457329},
journal = {Geomicrobiology Journal},
number = ,
volume = 24,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}
  • The reduction of Fe during bacterial anaerobic respiration in sediments and soils not only causes the degradation of organic matter but also results in changes in mineralogy and the redistribution of many nutrients and trace metals. Understanding trace metal patterns in sedimentary rocks and predicting the fate of contaminants in the environment requires a detailed understanding of the mechanisms through which they are redistributed during Fe reduction. In this work, lacustrine sediments from Lake Matano in Indonesia were incubated in a minimal media with the dissimilatory iron reducing (DIR) bacterium Shewanella putrefaciens 200R. These sediments were reductively dissolved at ratesmore » slower than pure synthetic goethite despite the presence of an 'easily reducible' component, as defined by selective extractions. DIR of the lacustrine sediments resulted in the substrate-dependent production of abundant quantities of extracellular polymeric substances. Trace elements, including Ni, Co, P, Si, and As, were released from the sediments with progressive Fe reduction while Cr was sequestered. Much of the initial trace metal mobility can be attributed to the rapid reduction of a Mn-rich oxyhydroxide phase. The production of organo-Fe(III) reveals that DIR bacteria can generate significant metal complexation capacity. This work demonstrates that DIR induces the release of many elements associated with Fe-Mn oxyhydroxides, despite secondary mineralization.« less
  • The toxicity and mobility of chromium can be diminished through its reduction from the hexavalent state to the trivalent form. Here we demonstrate a microbially mediated pathway for chromate reduction. Iron reducing bacteria, ubiquitous organisms within soils and sediments, stimulate chromate reduction by generating ferrous iron--a facile reductant of hexavalent chromium. Subsequent to reduction, Cr(III) then precipitates as a chromium hydroxide.
  • No abstract prepared.