Genome sequencing reveals complex secondary metabolome in themarine actinomycete Salinispora tropica
Recent fermentation studies have identified actinomycetes ofthe marine-dwelling genus Salinispora as prolific natural productproducers. To further evaluate their biosynthetic potential, we analyzedall identifiable secondary natural product gene clusters from therecently sequenced 5,184,724 bp S. tropica CNB-440 circular genome. Ouranalysis shows that biosynthetic potential meets or exceeds that shown byprevious Streptomyces genome sequences as well as other naturalproduct-producing actinomycetes. The S. tropica genome features ninepolyketide synthase systems of every known formally classified family,non-ribosomal peptide synthetases and several hybrid clusters. While afew clusters appear to encode molecules previously identified inStreptomyces species,the majority of the 15 biosynthetic loci are novel.Specific chemical information about putative and observed natural productmolecules is presented and discussed. In addition, our bioinformaticanalysis was critical for the structure elucidation of the novelpolyenemacrolactam salinilactam A. This study demonstrates the potentialfor genomic analysis to complement and strengthen traditional naturalproduct isolation studies and firmly establishes the genus Salinispora asa rich source of novel drug-like molecules.
- Research Organization:
- COLLABORATION - Scripps Institution ofOceanography
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 929322
- Report Number(s):
- LBNL-62942; R&D Project: 626882; BnR: KP1103010; TRN: US200813%%186
- Journal Information:
- Proceedings of the National Academy ofSciences, Vol. 104, Issue 25; Related Information: Journal Publication Date: June 19,2007
- Country of Publication:
- United States
- Language:
- English
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