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Title: Identification of genes directly regulated by the oncogene ZNF217using ChIP-chip assays.

Abstract

It has been proposed that ZNF217, which is amplified at 20q13 in various tumors, plays a key role during neoplastic transformation. ZNF217 has been purified in complexes that contain repressor proteins such as CtBP2, suggesting that it acts as a transcriptional repressor. However, the function of ZNF217 has not been well characterized due to a lack of known target genes. Using a global chromatin immunoprecipitation (ChIP)-chip approach, we identified thousands of ZNF217 binding sites in three tumor cell lines (MCF7, SW480, and Ntera2). Further analysis of ZNF217 in Ntera2 cells showed that many promoters are bound by ZNF217 and CtBP2 and that a subset of these promoters are activated upon removal of ZNF217. Thus, our in vivo studies corroborate the in vitro biochemical analyses of ZNF217-containing complexes and support the hypothesis that ZNF217 functions as a transcriptional repressor. Gene ontology analysis showed that ZNF217 targets in Ntera2 cells are involved in organ development, suggesting that one function of ZNF217 may be to repress differentiation. Accordingly we show that differentiation of Ntera2 cells with retinoic acid led to down-regulation of ZNF217. Our identification of thousands of ZNF217 target genes will enable further studies of the consequences of aberrant expression of ZNF217more » during neoplastic transformation.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
COLLABORATION - UCDavis
Sponsoring Org.:
USDOE
OSTI Identifier:
928017
Report Number(s):
LBNL-62763
R&D Project: L0246J; BnR: 400409900; TRN: US200816%%984
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: The Journal of Biological Chemistry; Journal Volume: 282; Journal Issue: 13; Related Information: Journal Publication Date: 03/30/2007
Country of Publication:
United States
Language:
English
Subject:
59; CHROMATIN; GENES; HYPOTHESIS; IN VITRO; IN VIVO; NEOPLASMS; ONCOGENES; ORGANS; PROMOTERS; PROTEINS; REMOVAL; RETINOIC ACID; TARGETS; TUMOR CELLS

Citation Formats

Krig, S.R., Jin, V.X., Bieda, M.C., O'geen, H., Yaswen, P., Green, R., and Farnham, P.J.. Identification of genes directly regulated by the oncogene ZNF217using ChIP-chip assays.. United States: N. p., 2007. Web. doi:10.1074/jbc.M611752200.
Krig, S.R., Jin, V.X., Bieda, M.C., O'geen, H., Yaswen, P., Green, R., & Farnham, P.J.. Identification of genes directly regulated by the oncogene ZNF217using ChIP-chip assays.. United States. doi:10.1074/jbc.M611752200.
Krig, S.R., Jin, V.X., Bieda, M.C., O'geen, H., Yaswen, P., Green, R., and Farnham, P.J.. Fri . "Identification of genes directly regulated by the oncogene ZNF217using ChIP-chip assays.". United States. doi:10.1074/jbc.M611752200.
@article{osti_928017,
title = {Identification of genes directly regulated by the oncogene ZNF217using ChIP-chip assays.},
author = {Krig, S.R. and Jin, V.X. and Bieda, M.C. and O'geen, H. and Yaswen, P. and Green, R. and Farnham, P.J.},
abstractNote = {It has been proposed that ZNF217, which is amplified at 20q13 in various tumors, plays a key role during neoplastic transformation. ZNF217 has been purified in complexes that contain repressor proteins such as CtBP2, suggesting that it acts as a transcriptional repressor. However, the function of ZNF217 has not been well characterized due to a lack of known target genes. Using a global chromatin immunoprecipitation (ChIP)-chip approach, we identified thousands of ZNF217 binding sites in three tumor cell lines (MCF7, SW480, and Ntera2). Further analysis of ZNF217 in Ntera2 cells showed that many promoters are bound by ZNF217 and CtBP2 and that a subset of these promoters are activated upon removal of ZNF217. Thus, our in vivo studies corroborate the in vitro biochemical analyses of ZNF217-containing complexes and support the hypothesis that ZNF217 functions as a transcriptional repressor. Gene ontology analysis showed that ZNF217 targets in Ntera2 cells are involved in organ development, suggesting that one function of ZNF217 may be to repress differentiation. Accordingly we show that differentiation of Ntera2 cells with retinoic acid led to down-regulation of ZNF217. Our identification of thousands of ZNF217 target genes will enable further studies of the consequences of aberrant expression of ZNF217 during neoplastic transformation.},
doi = {10.1074/jbc.M611752200},
journal = {The Journal of Biological Chemistry},
number = 13,
volume = 282,
place = {United States},
year = {Fri Jan 26 00:00:00 EST 2007},
month = {Fri Jan 26 00:00:00 EST 2007}
}