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Title: Community Proteogenomics: background and application to the Rifle Bioremediation project


No abstract prepared.

Publication Date:
Research Org.:
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
Report Number(s):
R&D Project: ERSD 1029580a; TRN: US0803156
Resource Type:
Resource Relation:
Conference: Annual Environmental Remediation Science Program (ERSP) Principal Investigator Meeting, April 16-19, 2007, Lansdowne, VA
Country of Publication:
United States

Citation Formats

Jill Banfield. Community Proteogenomics: background and application to the Rifle Bioremediation project. United States: N. p., 2007. Web.
Jill Banfield. Community Proteogenomics: background and application to the Rifle Bioremediation project. United States.
Jill Banfield. Thu . "Community Proteogenomics: background and application to the Rifle Bioremediation project". United States. doi:.
title = {Community Proteogenomics: background and application to the Rifle Bioremediation project},
author = {Jill Banfield},
abstractNote = {No abstract prepared.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Apr 19 00:00:00 EDT 2007},
month = {Thu Apr 19 00:00:00 EDT 2007}

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  • The Paraho Oil Shale Demonstration is a privately financed project sponsored by seventeen participants. The program to demonstrate the Paraho technology on oil shale is being conducted at the Anvil Points Experiment Station of the Energy Research and Development Administration (ERDA). The facility is located on the Naval Oil Shale Reserve near Rifle, Colorado. A $9 million, 34 month, mining, retorting and disposal program is scheduled for completion by mid 1976. A large pilot plant and a semi-works scale plant were erected and put into operation in 1974. Two modes of operation, direct and indirect heating, have been successfully demonstrated.more » Large scale refining of crude shale oil and retorted shale management experiments have been carried out. The extended retorting demonstration runs on Paraho's cylindrical, vertical shaft kilns have characterized this new technology as environmentally acceptable, with high liquid recovery, high thermal efficiency and low water consumption. The demonstration operations and the outlook for the commercial development of oil shale are discussed.« less
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  • Chemical measurements provide a means to evaluate crude oil and refined product bioremediation effectiveness in field and laboratory studies. These measurements are used to determine the net decrease in product or target compound concentrations in complex soil systems. The analytical methods used to evaluate these constituents will have a direct impact on the ability of the investigator to; (1) detect losses due to biodegradation, (2) understand the processes responsible for the hydrocarbon degradation and, (3) determine the rates of hydrocarbon degradation. This understanding is critical for the testing and design of bioremediation programs. While standard EPA methods are useful formore » measuring a wide variety of industrial and agrochemicals, they were not designed for the detection and accurate measurement of petroleum compounds. The chemical data generated with these standard methods are usually of limited utility because they lack the chemical specificity required to evaluate hydrocarbon compositional changes in the oil contamination required to evaluate biodegradation. The applications and limitations of standard EPA methodologies (EPA Methods 418.1, 8270, and modified 8015) will be evaluated and compared to several new analytical methods currently being used by the petroleum industry (e.g., gross compositional analysis, TLC-FID analysis, and enhanced EPA Method 8270) to evaluate bioremediation effectiveness in soils.« less
  • Clean up of the toxic legacy of the Cold War is projected to be the most expensive domestic project the nation has yet undertaken. Remediation of the Department of Energy and Department of Defense toxic waste sites alone are projected to cost {approximately}$1 trillion over a 20-30 year period. New, cost effective technologies are needed to attack this enormous problem. Los Alamos has put together a cross-divisional team of scientist to develop science based bioremediation technology to work toward this goal. In the team we have expertise in: (1) molecular, ecosystem and transport modeling; (2) genetic and protein engineering; (3)more » microbiology and microbial ecology; (4) structural biology; and (5) bioinorganic chemistry. This document summarizes talks at a workshop of different aspects of bioremediation technology including the following: Introducing novel function into a Heme enzyme: engineering by excavation; cytochrome P-450: ideal systems for bioremediation?; selection and development of bacterial strains for in situ remediation of cholorinated solvents; genetic analysis and preparation of toluene ortho-monooxygenase for field application in remediation of trichloroethylene; microbial ecology and diversity important to bioremediation; engineering haloalkane dehalogenase for bioremediation; enzymes for oxidative biodegradation; indigenous bacteria as hosts for engineered proteins; performance of indigenous bacterial, hosting engineered proteins in microbial communities.« less
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