Hexavalent chromium reduction in Desulfovibrio vulgarisHildenborough causes transitory inhibition of sulfate reduction and cellgrowth

Klonowska, A; Clark, M E; Thieman, S B; Giles, B J; Wall, J D; Fields, M W

Title: Hexavalent chromium reduction in Desulfovibrio vulgarisHildenborough causes transitory inhibition of sulfate reduction and cellgrowth

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Abstract

Desulfovibrio vulgaris Hildenborough is a well-studiedsulfate reducer that can reduce heavy metals and radionuclides [e.g.,Cr(VI) and U(VI)]. Cultures grown in a defined medium had a lag period ofapproximately 30 h when exposed to 0.05 mM Cr(VI). Substrate analysesrevealed that although Cr(VI) was reduced within the first 5 h, growthwas not observed for an additional 20 h. The growth lag could beexplained by a decline in cell viability; however, during this time smallamounts of lactate were still utilized without sulfate reduction oracetate formation. Approximately 40 h after Cr exposure (0.05 mM),sulfate reduction occurred concurrently with the accumulation of acetate.Similar amounts of hydrogen were produced by Cr-exposed cells compared tocontrol cells, and lactate was not converted to glycogen duringnon-growth conditions. D. vulgaris cells treated with a reducing agentand then exposed to Cr(VI) still experienced a growth lag, but theaddition of ascorbate at the time of Cr(VI) addition prevented the lagperiod. In addition, cells grown on pyruvate displayed more tolerance toCr(VI) compared to lactate-grown cells. These results indicated that D.vulgaris utilized lactate during Cr(VI) exposure without the reduction ofsulfate or production of acetate, and that ascorbate and pyruvate couldprotect D. vulgaris cells from Cr(VI)/Cr(III) toxicity.

Authors:: Klonowska, A; Clark, M E; Thieman, S B; Giles, B J; Wall, J D; Fields, M W

Publication Date:: Mon Jan 07 00:00:00 EST 2008

Research Org.:: COLLABORATION - MiamiU./Ohio

Sponsoring Org.:: USDOE Director. Office of Science. Biological andEnvironmental Research

OSTI Identifier:: 926558

Report Number(s):: LBNL-60502
R&D Project: VGTLMT; BnR: KP1501021

DOE Contract Number:: DE-AC02-05CH11231

Resource Type:: Journal Article

Journal Name:: Applied Microbiology and Biotechnology

Additional Journal Information:: Journal Volume: DOI 10.1007/s00253-008-1381-x; Related Information: Journal Publication Date: 12 February2008

Country of Publication:: United States

Language:: English

Subject:: 59; 54; Bioremediation Environmental Genomics Metabolism StressResponse Sulfate Reducers Desulfovibrio - Chromium - Celltoxicity

Citation Formats


                    Klonowska, A, Clark, M E, Thieman, S B, Giles, B J, Wall, J D, and Fields, M W. Hexavalent chromium reduction in Desulfovibrio vulgarisHildenborough causes transitory inhibition of sulfate reduction and cellgrowth.  United States: N. p., 2008. 
        Web.

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                    Klonowska, A, Clark, M E, Thieman, S B, Giles, B J, Wall, J D, & Fields, M W. Hexavalent chromium reduction in Desulfovibrio vulgarisHildenborough causes transitory inhibition of sulfate reduction and cellgrowth.  United States.

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                    Klonowska, A, Clark, M E, Thieman, S B, Giles, B J, Wall, J D, and Fields, M W. 2008.  
        "Hexavalent chromium reduction in Desulfovibrio vulgarisHildenborough causes transitory inhibition of sulfate reduction and cellgrowth".  United States.

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@article{osti_926558,

  title        = {Hexavalent chromium reduction in Desulfovibrio vulgarisHildenborough causes transitory inhibition of sulfate reduction and cellgrowth},

  author       = {Klonowska, A and Clark, M E and Thieman, S B and Giles, B J and Wall, J D and Fields, M W},

  abstractNote = {Desulfovibrio vulgaris Hildenborough is a well-studiedsulfate reducer that can reduce heavy metals and radionuclides [e.g.,Cr(VI) and U(VI)]. Cultures grown in a defined medium had a lag period ofapproximately 30 h when exposed to 0.05 mM Cr(VI). Substrate analysesrevealed that although Cr(VI) was reduced within the first 5 h, growthwas not observed for an additional 20 h. The growth lag could beexplained by a decline in cell viability; however, during this time smallamounts of lactate were still utilized without sulfate reduction oracetate formation. Approximately 40 h after Cr exposure (0.05 mM),sulfate reduction occurred concurrently with the accumulation of acetate.Similar amounts of hydrogen were produced by Cr-exposed cells compared tocontrol cells, and lactate was not converted to glycogen duringnon-growth conditions. D. vulgaris cells treated with a reducing agentand then exposed to Cr(VI) still experienced a growth lag, but theaddition of ascorbate at the time of Cr(VI) addition prevented the lagperiod. In addition, cells grown on pyruvate displayed more tolerance toCr(VI) compared to lactate-grown cells. These results indicated that D.vulgaris utilized lactate during Cr(VI) exposure without the reduction ofsulfate or production of acetate, and that ascorbate and pyruvate couldprotect D. vulgaris cells from Cr(VI)/Cr(III) toxicity.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/926558},
  journal      = {Applied Microbiology and Biotechnology},
number       = ,

  volume       = DOI 10.1007/s00253-008-1381-x,

  place        = {United States},

  year         = {Mon Jan 07 00:00:00 EST 2008},

  month        = {Mon Jan 07 00:00:00 EST 2008}

}

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