skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: 31 Flavors of Drosophila Rab proteins

Abstract

Rab proteins are small GTPases that play important roles intransport of vesicle cargo and recruitment, association of motor andother proteins with vesicles, and docking and fusion of vesicles atdefined locations. In vertebrates, more than 75 Rab genes have beenidentified, some of which have been intensively studied for their rolesin endosome and synaptic vesicle trafficking. Recent studies of thefunctions of certain Rab proteins have revealed specific roles inmediating developmental signal transduction. We have begun a systematicgenetic study of the 33 Rab genes in Drosophila. Most of the fly proteinsare clearly related to specific vertebrate proteins. We report here thecreation of a set of transgenic fly lines that allow spatially andtemporally regulated expression of Drosophila Rab proteins. We generatedfluorescent protein-tagged wild-type, dominant-negative, andconstitutively active forms of 31 Drosophila Rab proteins. We describeDrosophila Rab expression patterns during embryogenesis, the subcellularlocalization of some Rab proteins, and comparisons of the localization ofwild-type, dominant-negative, and constitutively active forms of selectedRab proteins. The high evolutionary conservation and low redundancy ofDrosophila Rab proteins make these transgenic lines a useful toolkit forinvestigating Rab functions in vivo.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
COLLABORATION - StanfordU.
OSTI Identifier:
923469
Report Number(s):
LBNL-62588
Journal ID: ISSN 0016-6731; GENTAE; R&D Project: L0111; BnR: 600305000; TRN: US200804%%1177
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Genetics; Journal Volume: 176; Journal Issue: 2; Related Information: Journal Publication Date: 06/2007
Country of Publication:
United States
Language:
English
Subject:
59; CARGO; DROSOPHILA; GENES; GENETICS; IN VIVO; MOTORS; PROTEINS; REDUNDANCY; TRANSPORT; VERTEBRATES

Citation Formats

Zhang, Jun, Schulze, Karen L., Hiesinger, P. Robin, Suyama, Kaye, Wang, Stream, Fish, Matthew, Acar, Melih, Hoskins, Roger A., Bellen, HugoJ., and Scott, Matthew P. 31 Flavors of Drosophila Rab proteins. United States: N. p., 2007. Web.
Zhang, Jun, Schulze, Karen L., Hiesinger, P. Robin, Suyama, Kaye, Wang, Stream, Fish, Matthew, Acar, Melih, Hoskins, Roger A., Bellen, HugoJ., & Scott, Matthew P. 31 Flavors of Drosophila Rab proteins. United States.
Zhang, Jun, Schulze, Karen L., Hiesinger, P. Robin, Suyama, Kaye, Wang, Stream, Fish, Matthew, Acar, Melih, Hoskins, Roger A., Bellen, HugoJ., and Scott, Matthew P. Tue . "31 Flavors of Drosophila Rab proteins". United States. doi:.
@article{osti_923469,
title = {31 Flavors of Drosophila Rab proteins},
author = {Zhang, Jun and Schulze, Karen L. and Hiesinger, P. Robin and Suyama, Kaye and Wang, Stream and Fish, Matthew and Acar, Melih and Hoskins, Roger A. and Bellen, HugoJ. and Scott, Matthew P.},
abstractNote = {Rab proteins are small GTPases that play important roles intransport of vesicle cargo and recruitment, association of motor andother proteins with vesicles, and docking and fusion of vesicles atdefined locations. In vertebrates, more than 75 Rab genes have beenidentified, some of which have been intensively studied for their rolesin endosome and synaptic vesicle trafficking. Recent studies of thefunctions of certain Rab proteins have revealed specific roles inmediating developmental signal transduction. We have begun a systematicgenetic study of the 33 Rab genes in Drosophila. Most of the fly proteinsare clearly related to specific vertebrate proteins. We report here thecreation of a set of transgenic fly lines that allow spatially andtemporally regulated expression of Drosophila Rab proteins. We generatedfluorescent protein-tagged wild-type, dominant-negative, andconstitutively active forms of 31 Drosophila Rab proteins. We describeDrosophila Rab expression patterns during embryogenesis, the subcellularlocalization of some Rab proteins, and comparisons of the localization ofwild-type, dominant-negative, and constitutively active forms of selectedRab proteins. The high evolutionary conservation and low redundancy ofDrosophila Rab proteins make these transgenic lines a useful toolkit forinvestigating Rab functions in vivo.},
doi = {},
journal = {Genetics},
number = 2,
volume = 176,
place = {United States},
year = {Tue Apr 03 00:00:00 EDT 2007},
month = {Tue Apr 03 00:00:00 EDT 2007}
}