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Title: Cellular Response of Shewanella oneidensis to StrontiumStress

Abstract

The physiology and transcriptome dynamics of the metalion-reducing bacterium Shewanella oneidensis strain MR-1 in response tononradioactive strontium (Sr) exposure were investigated. Studiesindicated that MR-1 was able to grow aerobically in complex medium in thepresence of 180 mMSrCl2 but showed severe growth inhibition at levelsabove that concentration. Temporal gene expression profiles weregenerated from aerobically grown, mid-exponential-phase MR-1 cellsshocked with 180 mM SrCl2 and analyzed for significant differences inmRNA abundance with reference to data for nonstressed MR-1 cells. Geneswith annotated functions in siderophore biosynthesis and iron transportwere among the most highly induced (>100-fold [P<0.05]) openreading frames in response to acute Sr stress, and a mutant(SO3032::pKNOCK) defective in siderophore production was found to behypersensitive to SrCl2 exposure, compared to parental and wild-typestrains. Transcripts encoding multidrug and heavy metal efflux pumps,proteins involved in osmotic adaptation, sulfate ABC transporters, andassimilative sulfur metabolism enzymes also were differentially expressedfollowing Sr exposure but at levels that were several orders of magnitudelower than those for iron transport genes. Precipitate formation wasobserved during aerobic growth of MR-1 in broth cultures amended with 50,100, or 150 mM SrCl2 but not in cultures of the SO3032::pKNOCK mutant orin the abiotic control. Chemical analysis of this precipitate usinglaser-induced breakdown spectroscopy and static secondarymore » ion massspectrometry indicated extracellular solid-phase sequestration of Sr,with at least a portion of the heavy metal associated with carbonatephases.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
COLLABORATION - ORNL
OSTI Identifier:
922807
Report Number(s):
LBNL-60417
Journal ID: ISSN 0099-2240; AEMIDF; R&D Project: VGTLAA; BnR: KP1102010; TRN: US200803%%534
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied and Environmental Microbiology; Journal Volume: 72; Journal Issue: 1; Related Information: Journal Publication Date: January,2006
Country of Publication:
United States
Language:
English
Subject:
59; BIOSYNTHESIS; CARBONATES; CHEMICAL ANALYSIS; ENZYMES; GENES; HEAVY METALS; INHIBITION; IRON; MASS SPECTROSCOPY; METABOLISM; MUTANTS; PHYSIOLOGY; PROTEINS; SPECTROSCOPY; STRONTIUM; SULFATES; SULFUR; Bioremediation Stress Response Transcriptomics

Citation Formats

Brown, Steven D., Martin, Madhavi, Deshpande, Sameer, Seal,Sudipta, Huang, Katherine, Alm, Eric, Yang, Yunfeng, Wu, Liyou, Yan,Tengfen, Liu, Xueduan, Arkin, Adam, Chourey, Karuna, Zhou, Jizhong, and Thompson, Dorothea K.. Cellular Response of Shewanella oneidensis to StrontiumStress. United States: N. p., 2007. Web.
Brown, Steven D., Martin, Madhavi, Deshpande, Sameer, Seal,Sudipta, Huang, Katherine, Alm, Eric, Yang, Yunfeng, Wu, Liyou, Yan,Tengfen, Liu, Xueduan, Arkin, Adam, Chourey, Karuna, Zhou, Jizhong, & Thompson, Dorothea K.. Cellular Response of Shewanella oneidensis to StrontiumStress. United States.
Brown, Steven D., Martin, Madhavi, Deshpande, Sameer, Seal,Sudipta, Huang, Katherine, Alm, Eric, Yang, Yunfeng, Wu, Liyou, Yan,Tengfen, Liu, Xueduan, Arkin, Adam, Chourey, Karuna, Zhou, Jizhong, and Thompson, Dorothea K.. Fri . "Cellular Response of Shewanella oneidensis to StrontiumStress". United States. doi:.
@article{osti_922807,
title = {Cellular Response of Shewanella oneidensis to StrontiumStress},
author = {Brown, Steven D. and Martin, Madhavi and Deshpande, Sameer and Seal,Sudipta and Huang, Katherine and Alm, Eric and Yang, Yunfeng and Wu, Liyou and Yan,Tengfen and Liu, Xueduan and Arkin, Adam and Chourey, Karuna and Zhou, Jizhong and Thompson, Dorothea K.},
abstractNote = {The physiology and transcriptome dynamics of the metalion-reducing bacterium Shewanella oneidensis strain MR-1 in response tononradioactive strontium (Sr) exposure were investigated. Studiesindicated that MR-1 was able to grow aerobically in complex medium in thepresence of 180 mMSrCl2 but showed severe growth inhibition at levelsabove that concentration. Temporal gene expression profiles weregenerated from aerobically grown, mid-exponential-phase MR-1 cellsshocked with 180 mM SrCl2 and analyzed for significant differences inmRNA abundance with reference to data for nonstressed MR-1 cells. Geneswith annotated functions in siderophore biosynthesis and iron transportwere among the most highly induced (>100-fold [P<0.05]) openreading frames in response to acute Sr stress, and a mutant(SO3032::pKNOCK) defective in siderophore production was found to behypersensitive to SrCl2 exposure, compared to parental and wild-typestrains. Transcripts encoding multidrug and heavy metal efflux pumps,proteins involved in osmotic adaptation, sulfate ABC transporters, andassimilative sulfur metabolism enzymes also were differentially expressedfollowing Sr exposure but at levels that were several orders of magnitudelower than those for iron transport genes. Precipitate formation wasobserved during aerobic growth of MR-1 in broth cultures amended with 50,100, or 150 mM SrCl2 but not in cultures of the SO3032::pKNOCK mutant orin the abiotic control. Chemical analysis of this precipitate usinglaser-induced breakdown spectroscopy and static secondary ion massspectrometry indicated extracellular solid-phase sequestration of Sr,with at least a portion of the heavy metal associated with carbonatephases.},
doi = {},
journal = {Applied and Environmental Microbiology},
number = 1,
volume = 72,
place = {United States},
year = {Fri Mar 30 00:00:00 EDT 2007},
month = {Fri Mar 30 00:00:00 EDT 2007}
}