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Title: Comparative Transcriptome Analysis of Desulfovibrio Vulgaris Grown in Planktonic Culture and Mature Biofilm on a Steel Surface

Abstract

The build-up of biofilms of sulphate -reducing bacteria (SRB) on metals surfaces may lead to severe corrosion of iron. To understand the processes at molecular level, in this study, a whole-genome oligonucleotide microarray was used to examine differential expression patterns between planktonic populations and mature biofilm of model SRB species Desulfovibrio vulgaris. Statistical analysis revealed that 472 genes were differentially expressed (1.5 fold or more with a p value less than 0.025) when comparing biofilm to planktonic cells. Among the differentially expressed genes were several that corresponded to biofilm formation genes identified in many aerobic bacterial biofilms (i.e., Pseudomonas species and Escherichia coli), such as down-regulation of genes encoding flagellin, flagellar motor switch protein and chemotaxis proteins involved in cell motility and induction of genes encoding sugar transferase and glycogen synthase involved in exopolysaccharide biosynthesis. In addition, D. vulgaris biofilm-bound cells exhibited decreased transcription of genes involved in protein synthesis, energy metabolism and sulfate reduction, as well as genes involved in general stress responses. These findings were all consistent with early suggestion that the average physiology of biofilm cells were similar to planktonic cells of stationary phases. Most notably, up-regulation of large number of outer membrane proteins was observed inmore » D. vulgaris biofilm. Although their function is still unknown, the higher expression of these genes in D. vulgaris biofilm could implicate important roles formation and maintenance of multi-cellular consortium on metal surface. The study provided insights into the metabolic networks associated with D. vulgaris biofilm formation and maintenance on an iron surface.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
921258
Report Number(s):
PNNL-SA-53026
Journal ID: ISSN 0175-7598; AMBIDG; TRN: US200804%%1524
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Applied Microbiology and Biotechnology, 76(2):447-457
Additional Journal Information:
Journal Volume: 76; Journal Issue: 2; Journal ID: ISSN 0175-7598
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; BIOSYNTHESIS; CORROSION; DESULFOVIBRIO; GENES; GLYCOGEN; IRON; MAINTENANCE; MEMBRANE PROTEINS; METABOLISM; MOTORS; OLIGONUCLEOTIDES; PHYSIOLOGY; PROTEINS; PSEUDOMONAS; SACCHAROSE; STEELS; SULFATE-REDUCING BACTERIA; SULFATES; SYNTHESIS; TRANSCRIPTION; TRANSFERASES; Transcriptome analysis; Biofilm; Corrosion; Desulfovibrio vulgaris

Citation Formats

Zhang, Weiwen, Culley, David E, Nie, Lei, and Scholten, Johannes C. Comparative Transcriptome Analysis of Desulfovibrio Vulgaris Grown in Planktonic Culture and Mature Biofilm on a Steel Surface. United States: N. p., 2007. Web. doi:10.1007/s00253-007-1014-9.
Zhang, Weiwen, Culley, David E, Nie, Lei, & Scholten, Johannes C. Comparative Transcriptome Analysis of Desulfovibrio Vulgaris Grown in Planktonic Culture and Mature Biofilm on a Steel Surface. United States. doi:10.1007/s00253-007-1014-9.
Zhang, Weiwen, Culley, David E, Nie, Lei, and Scholten, Johannes C. Wed . "Comparative Transcriptome Analysis of Desulfovibrio Vulgaris Grown in Planktonic Culture and Mature Biofilm on a Steel Surface". United States. doi:10.1007/s00253-007-1014-9.
@article{osti_921258,
title = {Comparative Transcriptome Analysis of Desulfovibrio Vulgaris Grown in Planktonic Culture and Mature Biofilm on a Steel Surface},
author = {Zhang, Weiwen and Culley, David E and Nie, Lei and Scholten, Johannes C},
abstractNote = {The build-up of biofilms of sulphate -reducing bacteria (SRB) on metals surfaces may lead to severe corrosion of iron. To understand the processes at molecular level, in this study, a whole-genome oligonucleotide microarray was used to examine differential expression patterns between planktonic populations and mature biofilm of model SRB species Desulfovibrio vulgaris. Statistical analysis revealed that 472 genes were differentially expressed (1.5 fold or more with a p value less than 0.025) when comparing biofilm to planktonic cells. Among the differentially expressed genes were several that corresponded to biofilm formation genes identified in many aerobic bacterial biofilms (i.e., Pseudomonas species and Escherichia coli), such as down-regulation of genes encoding flagellin, flagellar motor switch protein and chemotaxis proteins involved in cell motility and induction of genes encoding sugar transferase and glycogen synthase involved in exopolysaccharide biosynthesis. In addition, D. vulgaris biofilm-bound cells exhibited decreased transcription of genes involved in protein synthesis, energy metabolism and sulfate reduction, as well as genes involved in general stress responses. These findings were all consistent with early suggestion that the average physiology of biofilm cells were similar to planktonic cells of stationary phases. Most notably, up-regulation of large number of outer membrane proteins was observed in D. vulgaris biofilm. Although their function is still unknown, the higher expression of these genes in D. vulgaris biofilm could implicate important roles formation and maintenance of multi-cellular consortium on metal surface. The study provided insights into the metabolic networks associated with D. vulgaris biofilm formation and maintenance on an iron surface.},
doi = {10.1007/s00253-007-1014-9},
journal = {Applied Microbiology and Biotechnology, 76(2):447-457},
issn = {0175-7598},
number = 2,
volume = 76,
place = {United States},
year = {2007},
month = {8}
}