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Title: Trichomes of tobacco excrete zinc as zinc-substituted calciumcarbonate and other zinc-containing compounds

Abstract

Tobacco (Nicotiana tabacum L. cv Xanthi) plants were exposed to toxic levels of zinc (Zn). Zn exposure resulted in toxicity signs in plants, and these damages were partly reduced by a calcium (Ca) supplement. Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes. Exposure to Zn and Zn + Ca increased the trichome density and induced the production of Ca/Zn mineral grains on the head cells of trichomes. These grains were aggregates of submicrometer-sized crystals and poorly crystalline material and contained Ca as major element, along with subordinate amounts of Zn, manganese, potassium, chlorine, phosphorus, silicon, and magnesium. Micro x-ray diffraction revealed that the large majority of the grains were composed essentially of metal-substituted calcite (CaCO3). CaCO3 polymorphs (aragonite and vaterite) and CaC2O4 (Ca oxalate) mono- and dihydrate also were identified, either as an admixture to calcite or in separate grains. Some grains did not diffract, although they contained Ca, suggesting the presence of amorphous form of Ca. The presence of Zn-substituted calcite was confirmed by Zn K-edge micro-extended x-ray absorption fine structure spectroscopy. Zn bound to organic compounds and Zn-containing silica and phosphate were also identified by this technique. The proportion of Zn-substitutedmore » calcite relative to the other species increased with Ca exposure. The production of Zn-containing biogenic calcite and other Zn compounds through the trichomes is a novel mechanism involved in Zn detoxification. This study illustrates the potential of laterally resolved x-ray synchrotron radiation techniques to study biomineralization and metal homeostasis processes in plants.« less

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
COLLABORATION - U.Grenoble/France
OSTI Identifier:
919393
Report Number(s):
LBNL-62776
TRN: US0806369
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Plant Physiology; Journal Volume: 114; Related Information: Journal Publication Date: 07/2006
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; CALCITE; CALCIUM; CALCIUM CARBONATES; FINE STRUCTURE; ORGANIC COMPOUNDS; SYNCHROTRON RADIATION; TOBACCO; X-RAY DIFFRACTION; ZINC; advanced light source als

Citation Formats

Sarret, G., Harada, E., Choi, Y-E., Isaure, M.-P., Geoffroy, N., Fakra, S., Marcus, M.A., Birschwilks, M., Clemens, S., and Manceau, A. Trichomes of tobacco excrete zinc as zinc-substituted calciumcarbonate and other zinc-containing compounds. United States: N. p., 2006. Web.
Sarret, G., Harada, E., Choi, Y-E., Isaure, M.-P., Geoffroy, N., Fakra, S., Marcus, M.A., Birschwilks, M., Clemens, S., & Manceau, A. Trichomes of tobacco excrete zinc as zinc-substituted calciumcarbonate and other zinc-containing compounds. United States.
Sarret, G., Harada, E., Choi, Y-E., Isaure, M.-P., Geoffroy, N., Fakra, S., Marcus, M.A., Birschwilks, M., Clemens, S., and Manceau, A. Sun . "Trichomes of tobacco excrete zinc as zinc-substituted calciumcarbonate and other zinc-containing compounds". United States. doi:.
@article{osti_919393,
title = {Trichomes of tobacco excrete zinc as zinc-substituted calciumcarbonate and other zinc-containing compounds},
author = {Sarret, G. and Harada, E. and Choi, Y-E. and Isaure, M.-P. and Geoffroy, N. and Fakra, S. and Marcus, M.A. and Birschwilks, M. and Clemens, S. and Manceau, A.},
abstractNote = {Tobacco (Nicotiana tabacum L. cv Xanthi) plants were exposed to toxic levels of zinc (Zn). Zn exposure resulted in toxicity signs in plants, and these damages were partly reduced by a calcium (Ca) supplement. Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes. Exposure to Zn and Zn + Ca increased the trichome density and induced the production of Ca/Zn mineral grains on the head cells of trichomes. These grains were aggregates of submicrometer-sized crystals and poorly crystalline material and contained Ca as major element, along with subordinate amounts of Zn, manganese, potassium, chlorine, phosphorus, silicon, and magnesium. Micro x-ray diffraction revealed that the large majority of the grains were composed essentially of metal-substituted calcite (CaCO3). CaCO3 polymorphs (aragonite and vaterite) and CaC2O4 (Ca oxalate) mono- and dihydrate also were identified, either as an admixture to calcite or in separate grains. Some grains did not diffract, although they contained Ca, suggesting the presence of amorphous form of Ca. The presence of Zn-substituted calcite was confirmed by Zn K-edge micro-extended x-ray absorption fine structure spectroscopy. Zn bound to organic compounds and Zn-containing silica and phosphate were also identified by this technique. The proportion of Zn-substituted calcite relative to the other species increased with Ca exposure. The production of Zn-containing biogenic calcite and other Zn compounds through the trichomes is a novel mechanism involved in Zn detoxification. This study illustrates the potential of laterally resolved x-ray synchrotron radiation techniques to study biomineralization and metal homeostasis processes in plants.},
doi = {},
journal = {Plant Physiology},
number = ,
volume = 114,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}