skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Ion Mobility Spectrometry – Mass Spectrometry Performance Using Electrodynamic Ion Funnels and Elevated Drift Gas Pressures

Abstract

The ability of ion mobility spectrometry coupled with mass spectrometry (IMS-MS) to characterize biological mixtures has been illustrated over the past eight years. However, the challenges posed by the extreme complexity of many biological samples have demonstrated the need for higher resolution IMS-MS measurements. We have developed a higher resolution ESI-IMS-TOF MS by utilizing high pressure electrodynamic ion funnels at both ends of the IMS drift cell and operating the drift cell at an elevated pressure compared to a previous design. The ESI-IMS-TOF MS instrument consists of an ESI source, an hourglass ion funnel used for ion accumulation/injection into an 88 cm drift cell followed by a 10 cm ion funnel and a commercial orthogonal time-of-flight mass spectrometer providing high mass measurement accuracy. It was found that the rear (exit) ion funnel could be effectively operated as an extension of the drift cell when the DC fields were matched, allowing the instrument to have an effective drift region of 98 cm. Two differentially pumped quadrupole regions were used to couple the IMS and TOF MS to focus and minimize the ion transient time between the stages. The resolution of the instrument was evaluated at pressures ranging from 4 to12 Torrmore » and ion mobility drift voltages of 16 V/cm (4 Torr) to 43 V/cm (12 Torr). An increase in resolution from 55 to 80 was observed from 4 to 12 Torr nitrogen drift gas with no loss in sensitivity. Given the increased usage of ion funnels prior to ion mobility separations, additional attention was directed towards the influence of drift gas on the observed ion populations trapped and transmitted using an electrodynamic ion funnel. The choice of drift gas was shown to influence the degree of ion heating and relative trapping efficiency within the ion funnel.« less

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
919281
Report Number(s):
PNNL-SA-52620
Journal ID: ISSN 1044-0305; JAMSEF; 400412000; TRN: US200822%%12
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Journal of the American Society for Mass Spectrometry, 18(7):1176-1187
Additional Journal Information:
Journal Volume: 18; Journal Issue: 7; Journal ID: ISSN 1044-0305
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; ELECTRODYNAMICS; ION MOBILITY; MASS SPECTROSCOPY; PERFORMANCE; RESOLUTION; TIME-OF-FLIGHT MASS SPECTROMETERS; ION SPECTROSCOPY; DESIGN; BIOLOGICAL MATERIALS; Ion mobility spectrometry, electrodynamic ion funnel, IMS-MS

Citation Formats

Baker, Erin Shammel, Clowers, Brian H, Li, Fumin, Tang, Keqi, Tolmachev, Aleksey V, Prior, David C, Belov, Mikhail E, and Smith, Richard D. Ion Mobility Spectrometry – Mass Spectrometry Performance Using Electrodynamic Ion Funnels and Elevated Drift Gas Pressures. United States: N. p., 2007. Web. doi:10.1016/j.jasms.2007.03.031.
Baker, Erin Shammel, Clowers, Brian H, Li, Fumin, Tang, Keqi, Tolmachev, Aleksey V, Prior, David C, Belov, Mikhail E, & Smith, Richard D. Ion Mobility Spectrometry – Mass Spectrometry Performance Using Electrodynamic Ion Funnels and Elevated Drift Gas Pressures. United States. https://doi.org/10.1016/j.jasms.2007.03.031
Baker, Erin Shammel, Clowers, Brian H, Li, Fumin, Tang, Keqi, Tolmachev, Aleksey V, Prior, David C, Belov, Mikhail E, and Smith, Richard D. Thu . "Ion Mobility Spectrometry – Mass Spectrometry Performance Using Electrodynamic Ion Funnels and Elevated Drift Gas Pressures". United States. https://doi.org/10.1016/j.jasms.2007.03.031.
@article{osti_919281,
title = {Ion Mobility Spectrometry – Mass Spectrometry Performance Using Electrodynamic Ion Funnels and Elevated Drift Gas Pressures},
author = {Baker, Erin Shammel and Clowers, Brian H and Li, Fumin and Tang, Keqi and Tolmachev, Aleksey V and Prior, David C and Belov, Mikhail E and Smith, Richard D},
abstractNote = {The ability of ion mobility spectrometry coupled with mass spectrometry (IMS-MS) to characterize biological mixtures has been illustrated over the past eight years. However, the challenges posed by the extreme complexity of many biological samples have demonstrated the need for higher resolution IMS-MS measurements. We have developed a higher resolution ESI-IMS-TOF MS by utilizing high pressure electrodynamic ion funnels at both ends of the IMS drift cell and operating the drift cell at an elevated pressure compared to a previous design. The ESI-IMS-TOF MS instrument consists of an ESI source, an hourglass ion funnel used for ion accumulation/injection into an 88 cm drift cell followed by a 10 cm ion funnel and a commercial orthogonal time-of-flight mass spectrometer providing high mass measurement accuracy. It was found that the rear (exit) ion funnel could be effectively operated as an extension of the drift cell when the DC fields were matched, allowing the instrument to have an effective drift region of 98 cm. Two differentially pumped quadrupole regions were used to couple the IMS and TOF MS to focus and minimize the ion transient time between the stages. The resolution of the instrument was evaluated at pressures ranging from 4 to12 Torr and ion mobility drift voltages of 16 V/cm (4 Torr) to 43 V/cm (12 Torr). An increase in resolution from 55 to 80 was observed from 4 to 12 Torr nitrogen drift gas with no loss in sensitivity. Given the increased usage of ion funnels prior to ion mobility separations, additional attention was directed towards the influence of drift gas on the observed ion populations trapped and transmitted using an electrodynamic ion funnel. The choice of drift gas was shown to influence the degree of ion heating and relative trapping efficiency within the ion funnel.},
doi = {10.1016/j.jasms.2007.03.031},
url = {https://www.osti.gov/biblio/919281}, journal = {Journal of the American Society for Mass Spectrometry, 18(7):1176-1187},
issn = {1044-0305},
number = 7,
volume = 18,
place = {United States},
year = {2007},
month = {6}
}