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Title: Cell wide responses to low oxygen exposure in Desulfovibriovulgaris Hildenborough

Abstract

The responses of the anaerobic, sulfate-reducing Desulfovibrio vulgaris Hildenborough to low oxygen exposure (0.1% O{sub 2}) were monitored via transcriptomics and proteomics. Exposure to 0.1% O{sub 2} caused a decrease in growth rate without affecting viability. A concerted up regulation in the predicted peroxide stress response regulon (PerR) genes was observed in response to the 0.1% O{sub 2} exposure. Several of these candidates also showed increases in protein abundance. Among the remaining small number of transcript changes was the up regulation of the predicted transmembrane tetraheme cytochrome c3 complex. Other known oxidative stress response candidates remained unchanged during this low O{sub 2} exposure. To fully understand the results of the 0.1% O{sub 2} exposure, transcriptomics and proteomics data were collected for exposure to air using a similar experimental protocol. In contrast to the 0.1% O{sub 2} exposure, air exposure was detrimental to both the growth rate and viability and caused dramatic changes at both the transcriptome and proteome levels. Interestingly, the transcripts of the predicted PerR regulon genes were down regulated during air exposure. Our results highlight the differences in the cell wide response to low and high O{sub 2} levels of in D. vulgaris and suggest that while exposuremore » to air is highly detrimental to D. vulgaris, this bacterium can successfully cope with periodic exposure to low O{sub 2} levels in its environment.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
Sponsoring Org.:
USDOE Director, Office of Science
OSTI Identifier:
919269
Report Number(s):
LBNL-63377
Journal ID: ISSN 0021-9193; JOBAAY; TRN: US200822%%205
DOE Contract Number:
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Bacteriology; Journal Volume: 189; Journal Issue: 16; Related Information: Journal Publication Date: 08/2007
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ABUNDANCE; AIR; CYTOCHROMES; DESULFOVIBRIO; GENES; OXYGEN; PEROXIDES; PROTEINS; REGULATIONS; VIABILITY

Citation Formats

Mukhopadhyay, A., Redding, A., Joachimiak, M., Arkin, A., Borglin, S., Dehal, P., Chakraborty, R., Geller, J., Hazen, T., He, Q., Joyner, D., Martin, V., Wall, J., Yang, Z., Zhou, J., and Keasling, J. Cell wide responses to low oxygen exposure in Desulfovibriovulgaris Hildenborough. United States: N. p., 2007. Web. doi:10.1128/JB.00368-07.
Mukhopadhyay, A., Redding, A., Joachimiak, M., Arkin, A., Borglin, S., Dehal, P., Chakraborty, R., Geller, J., Hazen, T., He, Q., Joyner, D., Martin, V., Wall, J., Yang, Z., Zhou, J., & Keasling, J. Cell wide responses to low oxygen exposure in Desulfovibriovulgaris Hildenborough. United States. doi:10.1128/JB.00368-07.
Mukhopadhyay, A., Redding, A., Joachimiak, M., Arkin, A., Borglin, S., Dehal, P., Chakraborty, R., Geller, J., Hazen, T., He, Q., Joyner, D., Martin, V., Wall, J., Yang, Z., Zhou, J., and Keasling, J. Sun . "Cell wide responses to low oxygen exposure in Desulfovibriovulgaris Hildenborough". United States. doi:10.1128/JB.00368-07. https://www.osti.gov/servlets/purl/919269.
@article{osti_919269,
title = {Cell wide responses to low oxygen exposure in Desulfovibriovulgaris Hildenborough},
author = {Mukhopadhyay, A. and Redding, A. and Joachimiak, M. and Arkin, A. and Borglin, S. and Dehal, P. and Chakraborty, R. and Geller, J. and Hazen, T. and He, Q. and Joyner, D. and Martin, V. and Wall, J. and Yang, Z. and Zhou, J. and Keasling, J.},
abstractNote = {The responses of the anaerobic, sulfate-reducing Desulfovibrio vulgaris Hildenborough to low oxygen exposure (0.1% O{sub 2}) were monitored via transcriptomics and proteomics. Exposure to 0.1% O{sub 2} caused a decrease in growth rate without affecting viability. A concerted up regulation in the predicted peroxide stress response regulon (PerR) genes was observed in response to the 0.1% O{sub 2} exposure. Several of these candidates also showed increases in protein abundance. Among the remaining small number of transcript changes was the up regulation of the predicted transmembrane tetraheme cytochrome c3 complex. Other known oxidative stress response candidates remained unchanged during this low O{sub 2} exposure. To fully understand the results of the 0.1% O{sub 2} exposure, transcriptomics and proteomics data were collected for exposure to air using a similar experimental protocol. In contrast to the 0.1% O{sub 2} exposure, air exposure was detrimental to both the growth rate and viability and caused dramatic changes at both the transcriptome and proteome levels. Interestingly, the transcripts of the predicted PerR regulon genes were down regulated during air exposure. Our results highlight the differences in the cell wide response to low and high O{sub 2} levels of in D. vulgaris and suggest that while exposure to air is highly detrimental to D. vulgaris, this bacterium can successfully cope with periodic exposure to low O{sub 2} levels in its environment.},
doi = {10.1128/JB.00368-07},
journal = {Journal of Bacteriology},
number = 16,
volume = 189,
place = {United States},
year = {Sun Mar 11 00:00:00 EST 2007},
month = {Sun Mar 11 00:00:00 EST 2007}
}