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Title: Short Peptides Enhance Single Cell Adhesion and Viability onMicroarrays

Abstract

Single cell patterning holds important implications forbiology, biochemistry, biotechnology, medicine, and bioinformatics. Thechallenge for single cell patterning is to produce small islands hostingonly single cells and retaining their viability for a prolonged period oftime. This study demonstrated a surface engineering approach that uses acovalently bound short peptide as a mediator to pattern cells withimproved single cell adhesion and prolonged cellular viabilityon goldpatterned SiO2 substrates. The underlying hypothesis is that celladhesion is regulated bythe type, availability, and stability ofeffective cell adhesion peptides, and thus covalently bound shortpeptides would promote cell spreading and, thus, single cell adhesion andviability. The effectiveness of this approach and the underlyingmechanism for the increased probability of single cell adhesion andprolonged cell viability by short peptides were studied by comparingcellular behavior of human umbilical cord vein endothelial cells on threemodelsurfaces whose gold electrodes were immobilized with fibronectin,physically adsorbed Arg-Glu-Asp-Val-Tyr, and covalently boundLys-Arg-Glu-Asp-Val-Tyr, respectively. The surface chemistry and bindingproperties were characterized by reflectance Fourier transform infraredspectroscopy. Both short peptides were superior to fibronectin inproducing adhesion of only single cells, whereas the covalently boundpeptide also reduced apoptosis and necrosisof adhered cells. Controllingcell spreading by peptide binding domains to regulate apoptosis andviability represents a fundamental mechanism in cell-materialsinteraction and provides anmore » effective strategy in engineering arrays ofsingle cells.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
Sponsoring Org.:
USDOE Director, Office of Science. Office of Basic EnergySciences; National Institutes of Health (NIH)
OSTI Identifier:
918638
Report Number(s):
LBNL-62720
Journal ID: ISSN 0743-7463; LANGD5; R&D Project: 458121; BnR: KC0204016; TRN: US200819%%369
DOE Contract Number:  
DE-AC02-05CH11231; NSF:NSF-EEC 9529161; NIHR01GM075095
Resource Type:
Journal Article
Resource Relation:
Journal Name: Langmuir; Journal Volume: 23; Journal Issue: 8; Related Information: Journal Publication Date: April 10,2007
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; ADHESION; PEPTIDES; VIABILITY; MICROARRAY TECHNOLOGY; APOPTOSIS; Biosensors Cell adhesion FTIR BioMEMS self-assembledmonolayers

Citation Formats

Veiseh, Mandana, Veiseh, Omid, Martin, Michael C., Asphahani,Fareid, and Zhang, Miqin. Short Peptides Enhance Single Cell Adhesion and Viability onMicroarrays. United States: N. p., 2007. Web. doi:10.1021/la062849k.
Veiseh, Mandana, Veiseh, Omid, Martin, Michael C., Asphahani,Fareid, & Zhang, Miqin. Short Peptides Enhance Single Cell Adhesion and Viability onMicroarrays. United States. doi:10.1021/la062849k.
Veiseh, Mandana, Veiseh, Omid, Martin, Michael C., Asphahani,Fareid, and Zhang, Miqin. Fri . "Short Peptides Enhance Single Cell Adhesion and Viability onMicroarrays". United States. doi:10.1021/la062849k. https://www.osti.gov/servlets/purl/918638.
@article{osti_918638,
title = {Short Peptides Enhance Single Cell Adhesion and Viability onMicroarrays},
author = {Veiseh, Mandana and Veiseh, Omid and Martin, Michael C. and Asphahani,Fareid and Zhang, Miqin},
abstractNote = {Single cell patterning holds important implications forbiology, biochemistry, biotechnology, medicine, and bioinformatics. Thechallenge for single cell patterning is to produce small islands hostingonly single cells and retaining their viability for a prolonged period oftime. This study demonstrated a surface engineering approach that uses acovalently bound short peptide as a mediator to pattern cells withimproved single cell adhesion and prolonged cellular viabilityon goldpatterned SiO2 substrates. The underlying hypothesis is that celladhesion is regulated bythe type, availability, and stability ofeffective cell adhesion peptides, and thus covalently bound shortpeptides would promote cell spreading and, thus, single cell adhesion andviability. The effectiveness of this approach and the underlyingmechanism for the increased probability of single cell adhesion andprolonged cell viability by short peptides were studied by comparingcellular behavior of human umbilical cord vein endothelial cells on threemodelsurfaces whose gold electrodes were immobilized with fibronectin,physically adsorbed Arg-Glu-Asp-Val-Tyr, and covalently boundLys-Arg-Glu-Asp-Val-Tyr, respectively. The surface chemistry and bindingproperties were characterized by reflectance Fourier transform infraredspectroscopy. Both short peptides were superior to fibronectin inproducing adhesion of only single cells, whereas the covalently boundpeptide also reduced apoptosis and necrosisof adhered cells. Controllingcell spreading by peptide binding domains to regulate apoptosis andviability represents a fundamental mechanism in cell-materialsinteraction and provides an effective strategy in engineering arrays ofsingle cells.},
doi = {10.1021/la062849k},
journal = {Langmuir},
number = 8,
volume = 23,
place = {United States},
year = {Fri Jan 19 00:00:00 EST 2007},
month = {Fri Jan 19 00:00:00 EST 2007}
}