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Title: Respiration of metal (hydr)oxides by Shewanella and Geobacter: a key role for multihaem c-type cytochromes

Abstract

Dissimilatory reduction of metal (e.g. Fe, Mn) (hydr)oxides represents a challenge for microorganisms, as their cell envelopes are impermeable to metal (hydr)oxides that are poorly soluble in water. To overcome this physical barrier, the Gram-negative bacteria Shewanella oneidensis MR-1 and Geobactersulfurreducens have developed electron transfer (ET) strategies that require multihaem c-type cytochromes (c-Cyts). In S. oneidensis MR-1, multihaem c-Cyts CymA and MtrA are believed to transfer electrons from the inner membrane quinone/quinol pool through the periplasm to the outer membrane. The type II secretion system of S. oneidensis MR-1 has been implicated in the reduction of metal (hydr)oxides, most likely by translocating decahaem c-Cyts MtrC and OmcA across outer membrane to the surface of bacterial cells where they form a protein complex. The extracellular MtrC and OmcA can directly reduce solid metal (hydr)oxides. Likewise, outer membrane multihaem c-Cyts OmcE and OmcS of G. sulfurreducens are suggested to transfer electrons from outer membrane to type IV pili that are hypothesized to relay the electrons to solid metal (hydr)oxides. Thus, multihaem c-Cyts play critical roles in S. oneidensis MR-1-and G. sulfurreducens-mediated dissimilatory reduction of solid metal (hydr)oxides by facilitating ET across the bacterial cell envelope.

Authors:
; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
917956
Report Number(s):
PNNL-SA-56434
KP1501021; TRN: US200817%%1045
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Molecular Microbiology, 65(1):12-20
Additional Journal Information:
Journal Volume: 65; Journal Issue: 1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACTERIA; CYTOCHROMES; ELECTRON TRANSFER; ELECTRONS; MEMBRANES; MICROORGANISMS; PROTEINS; RESPIRATION; SECRETION; WATER; shewanella oneidensis MR-1; geobacter; respiration of metal; dissimilatory reduction

Citation Formats

Shi, Liang, Squier, Thomas C, Zachara, John M, and Fredrickson, Jim K. Respiration of metal (hydr)oxides by Shewanella and Geobacter: a key role for multihaem c-type cytochromes. United States: N. p., 2007. Web. doi:10.1111/j.1365-2958.2007.05783.x.
Shi, Liang, Squier, Thomas C, Zachara, John M, & Fredrickson, Jim K. Respiration of metal (hydr)oxides by Shewanella and Geobacter: a key role for multihaem c-type cytochromes. United States. doi:10.1111/j.1365-2958.2007.05783.x.
Shi, Liang, Squier, Thomas C, Zachara, John M, and Fredrickson, Jim K. Sun . "Respiration of metal (hydr)oxides by Shewanella and Geobacter: a key role for multihaem c-type cytochromes". United States. doi:10.1111/j.1365-2958.2007.05783.x.
@article{osti_917956,
title = {Respiration of metal (hydr)oxides by Shewanella and Geobacter: a key role for multihaem c-type cytochromes},
author = {Shi, Liang and Squier, Thomas C and Zachara, John M and Fredrickson, Jim K},
abstractNote = {Dissimilatory reduction of metal (e.g. Fe, Mn) (hydr)oxides represents a challenge for microorganisms, as their cell envelopes are impermeable to metal (hydr)oxides that are poorly soluble in water. To overcome this physical barrier, the Gram-negative bacteria Shewanella oneidensis MR-1 and Geobactersulfurreducens have developed electron transfer (ET) strategies that require multihaem c-type cytochromes (c-Cyts). In S. oneidensis MR-1, multihaem c-Cyts CymA and MtrA are believed to transfer electrons from the inner membrane quinone/quinol pool through the periplasm to the outer membrane. The type II secretion system of S. oneidensis MR-1 has been implicated in the reduction of metal (hydr)oxides, most likely by translocating decahaem c-Cyts MtrC and OmcA across outer membrane to the surface of bacterial cells where they form a protein complex. The extracellular MtrC and OmcA can directly reduce solid metal (hydr)oxides. Likewise, outer membrane multihaem c-Cyts OmcE and OmcS of G. sulfurreducens are suggested to transfer electrons from outer membrane to type IV pili that are hypothesized to relay the electrons to solid metal (hydr)oxides. Thus, multihaem c-Cyts play critical roles in S. oneidensis MR-1-and G. sulfurreducens-mediated dissimilatory reduction of solid metal (hydr)oxides by facilitating ET across the bacterial cell envelope.},
doi = {10.1111/j.1365-2958.2007.05783.x},
journal = {Molecular Microbiology, 65(1):12-20},
number = 1,
volume = 65,
place = {United States},
year = {2007},
month = {7}
}