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Title: Photoproduction of Hydrogen by Sulfur-Deprived Chlamydomonas reinhardtii Mutants with Impaired Photosystem II Photochemical Activity

Abstract

Photoproduction of H2 was examined in a series of sulfur-deprived Chlamydomonas reinhardtii D1-R323 mutants with progressively impaired PSII photochemical activity. In the R323H, R323D, and R323E D1 mutants, replacement of arginine affects photosystem II (PSII) function, as demonstrated by progressive decreases in O2-evolving activity and loss of PSII photochemical activity. Significant changes in PSII activity were found when the arginine residue was replaced by negatively charged amino acid residues (R323D and R323E). However, the R323H (positively charged or neutral, depending on the ambient pH) mutant had minimal changes in PSII activity. The R323H, R323D, and R323E mutants and the pseudo-wild-type (pWt) with restored PSII function were used to study the effects of sulfur deprivation on H2-production activity. All of these mutants exhibited significant changes in the normal parameters associated with the H2-photoproduction process, such as a shorter aerobic phase, lower accumulation of starch, a prolonged anaerobic phase observed before the onset of H2-production, a shorter duration of H2-production, lower H2 yields compared to the pWt control, and slightly higher production of dark fermentation products such as acetate and formate. The more compromised the PSII photochemical activity, the more dramatic was the effect of sulfur deprivation on the H2-production process, whichmore » depends both on the presence of residual PSII activity and the amount of stored starch.« less

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
915269
DOE Contract Number:  
AC36-99-GO10337
Resource Type:
Journal Article
Resource Relation:
Journal Name: Photosynthesis Research; Journal Volume: 94; Journal Issue: 1, 2007
Country of Publication:
United States
Language:
English
Subject:
08 HYDROGEN; 59 BASIC BIOLOGICAL SCIENCES; ACETATES; AMINO ACIDS; ARGININE; CHLAMYDOMONAS; FERMENTATION; HYDROGEN; MUTANTS; PHOTOPRODUCTION; PRODUCTION; RESIDUES; STARCH; SULFUR; Basic Sciences

Citation Formats

Makarova, V. V., Kosourov, S., Krendeleva, T. E., Semin, B. K., Kukarskikh, G. P., Rubin, A. B., Sayre, R. T., Ghirardi, M. L., and Seibert, M. Photoproduction of Hydrogen by Sulfur-Deprived Chlamydomonas reinhardtii Mutants with Impaired Photosystem II Photochemical Activity. United States: N. p., 2007. Web. doi:10.1007/s11120-007-9219-4.
Makarova, V. V., Kosourov, S., Krendeleva, T. E., Semin, B. K., Kukarskikh, G. P., Rubin, A. B., Sayre, R. T., Ghirardi, M. L., & Seibert, M. Photoproduction of Hydrogen by Sulfur-Deprived Chlamydomonas reinhardtii Mutants with Impaired Photosystem II Photochemical Activity. United States. doi:10.1007/s11120-007-9219-4.
Makarova, V. V., Kosourov, S., Krendeleva, T. E., Semin, B. K., Kukarskikh, G. P., Rubin, A. B., Sayre, R. T., Ghirardi, M. L., and Seibert, M. Mon . "Photoproduction of Hydrogen by Sulfur-Deprived Chlamydomonas reinhardtii Mutants with Impaired Photosystem II Photochemical Activity". United States. doi:10.1007/s11120-007-9219-4.
@article{osti_915269,
title = {Photoproduction of Hydrogen by Sulfur-Deprived Chlamydomonas reinhardtii Mutants with Impaired Photosystem II Photochemical Activity},
author = {Makarova, V. V. and Kosourov, S. and Krendeleva, T. E. and Semin, B. K. and Kukarskikh, G. P. and Rubin, A. B. and Sayre, R. T. and Ghirardi, M. L. and Seibert, M.},
abstractNote = {Photoproduction of H2 was examined in a series of sulfur-deprived Chlamydomonas reinhardtii D1-R323 mutants with progressively impaired PSII photochemical activity. In the R323H, R323D, and R323E D1 mutants, replacement of arginine affects photosystem II (PSII) function, as demonstrated by progressive decreases in O2-evolving activity and loss of PSII photochemical activity. Significant changes in PSII activity were found when the arginine residue was replaced by negatively charged amino acid residues (R323D and R323E). However, the R323H (positively charged or neutral, depending on the ambient pH) mutant had minimal changes in PSII activity. The R323H, R323D, and R323E mutants and the pseudo-wild-type (pWt) with restored PSII function were used to study the effects of sulfur deprivation on H2-production activity. All of these mutants exhibited significant changes in the normal parameters associated with the H2-photoproduction process, such as a shorter aerobic phase, lower accumulation of starch, a prolonged anaerobic phase observed before the onset of H2-production, a shorter duration of H2-production, lower H2 yields compared to the pWt control, and slightly higher production of dark fermentation products such as acetate and formate. The more compromised the PSII photochemical activity, the more dramatic was the effect of sulfur deprivation on the H2-production process, which depends both on the presence of residual PSII activity and the amount of stored starch.},
doi = {10.1007/s11120-007-9219-4},
journal = {Photosynthesis Research},
number = 1, 2007,
volume = 94,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}