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Title: Surface chemistries for antibody microarrays

Abstract

Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection of disease biomarkers. The original technology for printing ELISA microarray chips and capturing antibodies on slides was derived from the DNA microarray field. However, due to the need to maintain antibody structure and function when immobilized, surface chemistries used for DNA microarrays are not always appropriate for ELISA microarrays. In order to identify better surface chemistries for antibody capture, a number of commercial companies and academic research groups have developed new slide types that could improve antibody function in microarray applications. In this review we compare and contrast the commercially available slide chemistries, as well as highlight some promising recent advances in the field.

Authors:
; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
914677
Report Number(s):
PNNL-SA-54458
400412000; TRN: US200812%%229
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Frontiers in Bioscience, 12:3956-3964; Journal Volume: 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ANTIBODIES; DETECTION; DISEASES; DNA; ENZYME IMMUNOASSAY; ELISA; Antibody; Microarray; Solid Support; Review

Citation Formats

Seurynck-Servoss, Shannon L., Baird, Cheryl L., Rodland, Karin D., and Zangar, Richard C. Surface chemistries for antibody microarrays. United States: N. p., 2007. Web. doi:10.2741/2362.
Seurynck-Servoss, Shannon L., Baird, Cheryl L., Rodland, Karin D., & Zangar, Richard C. Surface chemistries for antibody microarrays. United States. doi:10.2741/2362.
Seurynck-Servoss, Shannon L., Baird, Cheryl L., Rodland, Karin D., and Zangar, Richard C. Tue . "Surface chemistries for antibody microarrays". United States. doi:10.2741/2362.
@article{osti_914677,
title = {Surface chemistries for antibody microarrays},
author = {Seurynck-Servoss, Shannon L. and Baird, Cheryl L. and Rodland, Karin D. and Zangar, Richard C.},
abstractNote = {Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection of disease biomarkers. The original technology for printing ELISA microarray chips and capturing antibodies on slides was derived from the DNA microarray field. However, due to the need to maintain antibody structure and function when immobilized, surface chemistries used for DNA microarrays are not always appropriate for ELISA microarrays. In order to identify better surface chemistries for antibody capture, a number of commercial companies and academic research groups have developed new slide types that could improve antibody function in microarray applications. In this review we compare and contrast the commercially available slide chemistries, as well as highlight some promising recent advances in the field.},
doi = {10.2741/2362},
journal = {Frontiers in Bioscience, 12:3956-3964},
number = ,
volume = 12,
place = {United States},
year = {Tue May 01 00:00:00 EDT 2007},
month = {Tue May 01 00:00:00 EDT 2007}
}