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Title: Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface

Abstract

The efficiency of sample ionization by electrospray ionization (ESI) and the transmission of the charged droplets and gas-phase ions through an ESI interface were investigated in order to advance the understanding of how these factors affect mass spectrometry (MS) sensitivity. In addition, the effects of the ES emitter distance to the inlet, solution flow rate, and inlet temperature to the ionization and transmission efficiency were characterized. Quantitative measurements of ES current loss throughout the ESI interface were accomplished by electrically isolating the front surface of the interface from the inner wall of the heated inlet capillary, enabling losses on the two surfaces to be distinguished. The ES current lost to the front surface of the ESI interface was also spatially profiled with a linear array of 340-µm-dia. electrodes placed adjacent to the inlet capillary entrance. Current transmitted as gas-phase ions was differentiated from charged droplets and solvent clusters by directly measuring sensitivity with a single quadrupole mass spectrometer. The study has revealed a large sampling efficiency into the inlet capillary (>90% at an emitter distance of 1 mm), a global rather than a local gas dynamic effect on the shape of the ES plume due to the gas flow conductancemore » limit of the inlet capillary, a large (>80%) loss of analyte after transmission through the inlet due to incomplete desolvation at a solution flow rate of 1.0 µL/min, and a decrease in analyte peak intensity at lower temperatures, despite a large increase in ES current transmission efficiency. These studies provide a clearer understanding of the parameters affecting ion transmission into the mass spectrometer, and will serve to guide the design of more efficient instrument interfaces.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
914674
Report Number(s):
PNNL-SA-53862
Journal ID: ISSN 1044-0305; JAMSEF; 400412000; TRN: US200812%%17
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Journal of the American Society for Mass Spectrometry, 18(9):1582-1590
Additional Journal Information:
Journal Volume: 18; Journal Issue: 9; Journal ID: ISSN 1044-0305
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; DESIGN; EFFICIENCY; ELECTRODES; IONIZATION; MASS SPECTROMETERS; MASS SPECTROSCOPY; SENSITIVITY; SAMPLE PREPARATION; MASS TRANSFER; EQUIPMENT INTERFACES

Citation Formats

Page, Jason S, Kelly, Ryan T, Tang, Keqi, and Smith, Richard D. Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface. United States: N. p., 2007. Web. doi:10.1016/j.jasms.2007.05.018.
Page, Jason S, Kelly, Ryan T, Tang, Keqi, & Smith, Richard D. Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface. United States. https://doi.org/10.1016/j.jasms.2007.05.018
Page, Jason S, Kelly, Ryan T, Tang, Keqi, and Smith, Richard D. Sat . "Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface". United States. https://doi.org/10.1016/j.jasms.2007.05.018.
@article{osti_914674,
title = {Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface},
author = {Page, Jason S and Kelly, Ryan T and Tang, Keqi and Smith, Richard D},
abstractNote = {The efficiency of sample ionization by electrospray ionization (ESI) and the transmission of the charged droplets and gas-phase ions through an ESI interface were investigated in order to advance the understanding of how these factors affect mass spectrometry (MS) sensitivity. In addition, the effects of the ES emitter distance to the inlet, solution flow rate, and inlet temperature to the ionization and transmission efficiency were characterized. Quantitative measurements of ES current loss throughout the ESI interface were accomplished by electrically isolating the front surface of the interface from the inner wall of the heated inlet capillary, enabling losses on the two surfaces to be distinguished. The ES current lost to the front surface of the ESI interface was also spatially profiled with a linear array of 340-µm-dia. electrodes placed adjacent to the inlet capillary entrance. Current transmitted as gas-phase ions was differentiated from charged droplets and solvent clusters by directly measuring sensitivity with a single quadrupole mass spectrometer. The study has revealed a large sampling efficiency into the inlet capillary (>90% at an emitter distance of 1 mm), a global rather than a local gas dynamic effect on the shape of the ES plume due to the gas flow conductance limit of the inlet capillary, a large (>80%) loss of analyte after transmission through the inlet due to incomplete desolvation at a solution flow rate of 1.0 µL/min, and a decrease in analyte peak intensity at lower temperatures, despite a large increase in ES current transmission efficiency. These studies provide a clearer understanding of the parameters affecting ion transmission into the mass spectrometer, and will serve to guide the design of more efficient instrument interfaces.},
doi = {10.1016/j.jasms.2007.05.018},
url = {https://www.osti.gov/biblio/914674}, journal = {Journal of the American Society for Mass Spectrometry, 18(9):1582-1590},
issn = {1044-0305},
number = 9,
volume = 18,
place = {United States},
year = {2007},
month = {9}
}