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Title: Silk Fibroin as an Organic Polymer for Controlled Drug Delivery

Abstract

The pharmaceutical utility of silk fibroin (SF) materials for drug delivery was investigated. SF films were prepared from aqueous solutions of the fibroin protein polymer and crystallinity was induced and controlled by methanol treatment. Dextrans of different molecular weights, as well as proteins, were physically entrapped into the drug delivery device during processing into films. Drug release kinetics were evaluated as a function of dextran molecular weight, and film crystallinity. Treatment with methanol resulted in an increase in {beta}-sheet structure, an increase in crystallinity and an increase in film surface hydrophobicity determined by FTIR, X-ray and contact angle techniques, respectively. The increase in crystallinity resulted in the sustained release of dextrans of molecular weights ranging from 4 to 40 kDa, whereas for less crystalline films sustained release was confined to the 40 kDa dextran. Protein release from the films was studied with horseradish peroxidase (HRP) and lysozyme (Lys) as model compounds. Enzyme release from the less crystalline films resulted in a biphasic release pattern, characterized by an initial release within the first 36 h, followed by a lag phase and continuous release between days 3 and 11. No initial burst was observed for films with higher crystallinity and subsequent releasemore » patterns followed linear kinetics for HRP, or no substantial release for Lys. In conclusion, SF is an interesting polymer for drug delivery of polysaccharides and bioactive proteins due to the controllable level of crystallinity and the ability to process the biomaterial in biocompatible fashion under ambient conditions to avoid damage to labile compounds to be delivered.« less

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
914328
Report Number(s):
BNL-78896-2007-JA
TRN: US200809%%181
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Controlled Release; Journal Volume: 111; Journal Issue: 1-2
Country of Publication:
United States
Language:
English
Subject:
10 SYNTHETIC FUELS; AQUEOUS SOLUTIONS; DEXTRAN; DRUGS; ENZYMES; KINETICS; LYSOZYME; METHANOL; MOLECULAR WEIGHT; ORGANIC POLYMERS; PEROXIDASES; POLYMERS; POLYSACCHARIDES; PROCESSING; PROTEINS; national synchrotron light source

Citation Formats

Hofmann,S., Wong Po Foo, C., Rossetti, F., Textor, M., Vunjak-Novakovic, G., Kaplan, D., Merkle, H., and Meinel, L.. Silk Fibroin as an Organic Polymer for Controlled Drug Delivery. United States: N. p., 2006. Web. doi:10.1016/j.jconrel.2005.12.009.
Hofmann,S., Wong Po Foo, C., Rossetti, F., Textor, M., Vunjak-Novakovic, G., Kaplan, D., Merkle, H., & Meinel, L.. Silk Fibroin as an Organic Polymer for Controlled Drug Delivery. United States. doi:10.1016/j.jconrel.2005.12.009.
Hofmann,S., Wong Po Foo, C., Rossetti, F., Textor, M., Vunjak-Novakovic, G., Kaplan, D., Merkle, H., and Meinel, L.. Sun . "Silk Fibroin as an Organic Polymer for Controlled Drug Delivery". United States. doi:10.1016/j.jconrel.2005.12.009.
@article{osti_914328,
title = {Silk Fibroin as an Organic Polymer for Controlled Drug Delivery},
author = {Hofmann,S. and Wong Po Foo, C. and Rossetti, F. and Textor, M. and Vunjak-Novakovic, G. and Kaplan, D. and Merkle, H. and Meinel, L.},
abstractNote = {The pharmaceutical utility of silk fibroin (SF) materials for drug delivery was investigated. SF films were prepared from aqueous solutions of the fibroin protein polymer and crystallinity was induced and controlled by methanol treatment. Dextrans of different molecular weights, as well as proteins, were physically entrapped into the drug delivery device during processing into films. Drug release kinetics were evaluated as a function of dextran molecular weight, and film crystallinity. Treatment with methanol resulted in an increase in {beta}-sheet structure, an increase in crystallinity and an increase in film surface hydrophobicity determined by FTIR, X-ray and contact angle techniques, respectively. The increase in crystallinity resulted in the sustained release of dextrans of molecular weights ranging from 4 to 40 kDa, whereas for less crystalline films sustained release was confined to the 40 kDa dextran. Protein release from the films was studied with horseradish peroxidase (HRP) and lysozyme (Lys) as model compounds. Enzyme release from the less crystalline films resulted in a biphasic release pattern, characterized by an initial release within the first 36 h, followed by a lag phase and continuous release between days 3 and 11. No initial burst was observed for films with higher crystallinity and subsequent release patterns followed linear kinetics for HRP, or no substantial release for Lys. In conclusion, SF is an interesting polymer for drug delivery of polysaccharides and bioactive proteins due to the controllable level of crystallinity and the ability to process the biomaterial in biocompatible fashion under ambient conditions to avoid damage to labile compounds to be delivered.},
doi = {10.1016/j.jconrel.2005.12.009},
journal = {J. Controlled Release},
number = 1-2,
volume = 111,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}