skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Ligand-Induced Asymmetry in Histidine Sensor Kinase Complex Regulates Quorum Sensing

Abstract

Bacteria sense their environment using receptors of the histidine sensor kinase family, but how kinase activity is regulated by ligand binding is not well understood. Autoinducer-2 (AI-2), a secreted signaling molecule originally identified in studies of the marine bacterium Vibrio harveyi, regulates quorum-sensing responses and allows communication between different bacterial species. AI-2 signal transduction in V. harveyi requires the integral membrane receptor LuxPQ, comprised of periplasmic binding protein (LuxP) and histidine sensor kinase (LuxQ) subunits. Combined X-ray crystallographic and functional studies show that AI-2 binding causes a major conformational change within LuxP, which in turn stabilizes a quaternary arrangement in which two LuxPQ monomers are asymmetrically associated. We propose that formation of this asymmetric quaternary structure is responsible for repressing the kinase activity of both LuxQ subunits and triggering the transition of V. harveyi into quorum-sensing mode.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
914285
Report Number(s):
BNL-78853-2007-JA
Journal ID: ISSN 0092-8674; CELLB5; TRN: US200809%%144
DOE Contract Number:  
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Cell; Journal Volume: 126; Journal Issue: 6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ASYMMETRY; BACTERIA; COMMUNICATIONS; CONFORMATIONAL CHANGES; FUNCTIONALS; HISTIDINE; MEMBRANES; MONOMERS; PHOSPHOTRANSFERASES; PROTEINS; national synchrotron light source

Citation Formats

Neiditch,M., Federle, M., Pompeani, A., Kelly, R., Swem, D., Jeffrey, P., Bassler, B., and Hughson, F. Ligand-Induced Asymmetry in Histidine Sensor Kinase Complex Regulates Quorum Sensing. United States: N. p., 2006. Web. doi:10.1016/j.cell.2006.07.032.
Neiditch,M., Federle, M., Pompeani, A., Kelly, R., Swem, D., Jeffrey, P., Bassler, B., & Hughson, F. Ligand-Induced Asymmetry in Histidine Sensor Kinase Complex Regulates Quorum Sensing. United States. doi:10.1016/j.cell.2006.07.032.
Neiditch,M., Federle, M., Pompeani, A., Kelly, R., Swem, D., Jeffrey, P., Bassler, B., and Hughson, F. Sun . "Ligand-Induced Asymmetry in Histidine Sensor Kinase Complex Regulates Quorum Sensing". United States. doi:10.1016/j.cell.2006.07.032.
@article{osti_914285,
title = {Ligand-Induced Asymmetry in Histidine Sensor Kinase Complex Regulates Quorum Sensing},
author = {Neiditch,M. and Federle, M. and Pompeani, A. and Kelly, R. and Swem, D. and Jeffrey, P. and Bassler, B. and Hughson, F.},
abstractNote = {Bacteria sense their environment using receptors of the histidine sensor kinase family, but how kinase activity is regulated by ligand binding is not well understood. Autoinducer-2 (AI-2), a secreted signaling molecule originally identified in studies of the marine bacterium Vibrio harveyi, regulates quorum-sensing responses and allows communication between different bacterial species. AI-2 signal transduction in V. harveyi requires the integral membrane receptor LuxPQ, comprised of periplasmic binding protein (LuxP) and histidine sensor kinase (LuxQ) subunits. Combined X-ray crystallographic and functional studies show that AI-2 binding causes a major conformational change within LuxP, which in turn stabilizes a quaternary arrangement in which two LuxPQ monomers are asymmetrically associated. We propose that formation of this asymmetric quaternary structure is responsible for repressing the kinase activity of both LuxQ subunits and triggering the transition of V. harveyi into quorum-sensing mode.},
doi = {10.1016/j.cell.2006.07.032},
journal = {Cell},
number = 6,
volume = 126,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}