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Title: Structure of Human Ferritin L Chain

Abstract

Ferritin is the major iron-storage protein present in all cells. It generally contains 24 subunits, with different ratios of heavy chain (H) to light chain (L), in the shape of a hollow sphere hosting up to 4500 ferric Fe atoms inside. H-rich ferritins catalyze the oxidation of iron(II), while L-rich ferritins promote the nucleation and storage of iron(III). Several X-ray structures have been determined, including those of L-chain ferritins from horse spleen (HoSF), recombinant L-chain ferritins from horse (HoLF), mouse (MoLF) and bullfrog (BfLF) as well as recombinant human H-chain ferritin (HuHF). Here, structures have been determined of two crystal forms of recombinant human L-chain ferritin (HuLF) obtained from native and perdeuterated proteins. The structures show a cluster of acidic residues at the ferrihydrite nucleation site and at the iron channel along the threefold axis. An ordered Cd{sup 2+} structure is observed within the iron channel, offering further insight into the route and mechanism of iron transport into the capsid. The loop between helices D and E, which is disordered in many other L-chain structures, is clearly visible in these two structures. The crystals generated from perdeuterated HuLF will be used for neutron diffraction studies.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
914142
Report Number(s):
BNL-78710-2007-JA
TRN: US0801571
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Cryst. D; Journal Volume: 62
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; ATOMS; CHAINS; FERRITIN; HORSES; IRON; NEUTRON DIFFRACTION; NUCLEATION; OXIDATION; PROTEINS; RESIDUES; SHAPE; SPLEEN; STORAGE; TRANSPORT; NSLS; national synchrotron light source

Citation Formats

Wang,Z., Li, C., Ellenburg, M., Soistman, E., Ruble, J., Wright, B., Ho, J., and Carter, D. Structure of Human Ferritin L Chain. United States: N. p., 2006. Web. doi:10.1107/S0907444906018294.
Wang,Z., Li, C., Ellenburg, M., Soistman, E., Ruble, J., Wright, B., Ho, J., & Carter, D. Structure of Human Ferritin L Chain. United States. doi:10.1107/S0907444906018294.
Wang,Z., Li, C., Ellenburg, M., Soistman, E., Ruble, J., Wright, B., Ho, J., and Carter, D. Sun . "Structure of Human Ferritin L Chain". United States. doi:10.1107/S0907444906018294.
@article{osti_914142,
title = {Structure of Human Ferritin L Chain},
author = {Wang,Z. and Li, C. and Ellenburg, M. and Soistman, E. and Ruble, J. and Wright, B. and Ho, J. and Carter, D.},
abstractNote = {Ferritin is the major iron-storage protein present in all cells. It generally contains 24 subunits, with different ratios of heavy chain (H) to light chain (L), in the shape of a hollow sphere hosting up to 4500 ferric Fe atoms inside. H-rich ferritins catalyze the oxidation of iron(II), while L-rich ferritins promote the nucleation and storage of iron(III). Several X-ray structures have been determined, including those of L-chain ferritins from horse spleen (HoSF), recombinant L-chain ferritins from horse (HoLF), mouse (MoLF) and bullfrog (BfLF) as well as recombinant human H-chain ferritin (HuHF). Here, structures have been determined of two crystal forms of recombinant human L-chain ferritin (HuLF) obtained from native and perdeuterated proteins. The structures show a cluster of acidic residues at the ferrihydrite nucleation site and at the iron channel along the threefold axis. An ordered Cd{sup 2+} structure is observed within the iron channel, offering further insight into the route and mechanism of iron transport into the capsid. The loop between helices D and E, which is disordered in many other L-chain structures, is clearly visible in these two structures. The crystals generated from perdeuterated HuLF will be used for neutron diffraction studies.},
doi = {10.1107/S0907444906018294},
journal = {Acta Cryst. D},
number = ,
volume = 62,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}