skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Hypomorphic mutations in the larval photokinesis A (lphA) gene have stage-specific effects on visual system function in Drosophila melanogaster

Abstract

Of the many genes that are expressed in the visual system of Drosophila melanogaster adults, some affect larval vision. However, with the exception of one X-linked mutation, no genes that have larval-specific effects on visual system structure or function have previously been reported. We describe the isolation and characterization of two mutant alleles that define the larval photokinesis A (lphA) gene, one allele of which is associated with a P-element insertion at cytogenetic locus 8E1-10. Larvae that express lphA mutations are, like normal animals, negatively photokinetic, but they are less responsive to white light than lphA+ controls. Larvae that are heterozygous in trans for a mutant allele and a deficiency that uncovers the lphA locus are blind, which indicates that the mutant allele is hypomorphic. lphA larvae respond normally to odorants and taste stimuli. Moreover, the lphA mutations do not affect adult flies` fast phototaxis or visually driven aspects of male sexual behavior, and electroretinograms recorded from the compound eyes of lphA/deficiency heterozygotes and lphA{sup 1}/lphA{sup 2} females are normal. These observations suggest that the lphA gene affects a larval-specific aspect of visual system function. 27 refs., 2 figs., 4 tabs.

Authors:
; ;  [1]
  1. Temple Univ., Philadelphia, PA (United States)
Publication Date:
OSTI Identifier:
91164
Resource Type:
Journal Article
Journal Name:
Genetics
Additional Journal Information:
Journal Volume: 139; Journal Issue: 4; Other Information: PBD: Apr 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DROSOPHILA; LARVAE; BEHAVIOR; PHYSIOLOGY; SENSE ORGANS; GENES; STRUCTURE-ACTIVITY RELATIONSHIPS; ANIMAL CELLS; GENE MUTATIONS; PHENOTYPE; DNA HYBRIDIZATION; MUTAGENESIS

Citation Formats

Gordesky-Gold, B, Warrick, J M, and Bixler, A. Hypomorphic mutations in the larval photokinesis A (lphA) gene have stage-specific effects on visual system function in Drosophila melanogaster. United States: N. p., 1995. Web.
Gordesky-Gold, B, Warrick, J M, & Bixler, A. Hypomorphic mutations in the larval photokinesis A (lphA) gene have stage-specific effects on visual system function in Drosophila melanogaster. United States.
Gordesky-Gold, B, Warrick, J M, and Bixler, A. Sat . "Hypomorphic mutations in the larval photokinesis A (lphA) gene have stage-specific effects on visual system function in Drosophila melanogaster". United States.
@article{osti_91164,
title = {Hypomorphic mutations in the larval photokinesis A (lphA) gene have stage-specific effects on visual system function in Drosophila melanogaster},
author = {Gordesky-Gold, B and Warrick, J M and Bixler, A},
abstractNote = {Of the many genes that are expressed in the visual system of Drosophila melanogaster adults, some affect larval vision. However, with the exception of one X-linked mutation, no genes that have larval-specific effects on visual system structure or function have previously been reported. We describe the isolation and characterization of two mutant alleles that define the larval photokinesis A (lphA) gene, one allele of which is associated with a P-element insertion at cytogenetic locus 8E1-10. Larvae that express lphA mutations are, like normal animals, negatively photokinetic, but they are less responsive to white light than lphA+ controls. Larvae that are heterozygous in trans for a mutant allele and a deficiency that uncovers the lphA locus are blind, which indicates that the mutant allele is hypomorphic. lphA larvae respond normally to odorants and taste stimuli. Moreover, the lphA mutations do not affect adult flies` fast phototaxis or visually driven aspects of male sexual behavior, and electroretinograms recorded from the compound eyes of lphA/deficiency heterozygotes and lphA{sup 1}/lphA{sup 2} females are normal. These observations suggest that the lphA gene affects a larval-specific aspect of visual system function. 27 refs., 2 figs., 4 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/91164}, journal = {Genetics},
number = 4,
volume = 139,
place = {United States},
year = {1995},
month = {4}
}