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Title: Microbial Studies Supporting Implementation of In Situ Bioremediation at TAN

Abstract

The Idaho National Engineering and Environmental Laboratory is evaluating in situ bioremediation of contaminated groundwater at its Test Area North Facility. To determine feasibility, microcosm and bioreactor studies were conducted to ascertain the ability of indigenous microbes to convert trichloroethene and dichloroethene to non-hazardous byproducts under aerobic and anaerobic conditions, and to measure the kinetics of microbial reactions associated with the degradation process. Microcosms were established from core samples and groundwater obtained from within the contaminant plume. These microcosms were amended with nutrients, under aerobic and anaerobic conditions, to identify electron donors capable of stimulating the degradation process. Results of the anaerobic microcosm studies showed that lactate, acetate and propionate amendments stimulated indigenous cell growth and functioned as effective substrates for reductive degradation of chloroethenes. Bioreactors inoculated with cultures from these anaerobic microcosms were operated under a batch mode for 42 days then converted to a fed-batch mode and operated at a 53-day hydraulic residence time. It was demonstrated that indigenous microbes capable of complete anaerobic reductive dechlorination are present in the subject well. It was also demonstrated that aerobic microbes capable of oxidizing chlorinated compounds produced by anaerobic reductive dechlorination are present. Kinetic data suggest that controlling the typemore » and concentration of electron donors can increase trichlorethene conversion rates. In the event that complete mineralization of trichlorethene does not occur following stimulation, and anaerobic/aerobic treatment scheme is feasible.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Idaho National Laboratory (INL)
Sponsoring Org.:
USDOE
OSTI Identifier:
911020
Report Number(s):
INEEL/EXT-98-00474
TRN: US200820%%232
DOE Contract Number:  
DE-AC07-99ID-13727
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
99 - GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; ACETATES; ANAEROBIC CONDITIONS; BINDING ENERGY; BIOREACTORS; BIOREMEDIATION; DECHLORINATION; ELECTRONS; HYDRAULICS; IMPLEMENTATION; INEEL; KINETICS; MICROCOSMS; MINERALIZATION; NUTRIENTS; STIMULATION; SUBSTRATES; VALENCE; bioreactor; bioremediation; groundwater; In situ; microcosm; Test Area North

Citation Formats

Barnes, Joan Marie, Matthern, Gretchen Elise, Rae, Catherine, and Ely, R L. Microbial Studies Supporting Implementation of In Situ Bioremediation at TAN. United States: N. p., 2000. Web. doi:10.2172/911020.
Barnes, Joan Marie, Matthern, Gretchen Elise, Rae, Catherine, & Ely, R L. Microbial Studies Supporting Implementation of In Situ Bioremediation at TAN. United States. doi:10.2172/911020.
Barnes, Joan Marie, Matthern, Gretchen Elise, Rae, Catherine, and Ely, R L. Wed . "Microbial Studies Supporting Implementation of In Situ Bioremediation at TAN". United States. doi:10.2172/911020. https://www.osti.gov/servlets/purl/911020.
@article{osti_911020,
title = {Microbial Studies Supporting Implementation of In Situ Bioremediation at TAN},
author = {Barnes, Joan Marie and Matthern, Gretchen Elise and Rae, Catherine and Ely, R L},
abstractNote = {The Idaho National Engineering and Environmental Laboratory is evaluating in situ bioremediation of contaminated groundwater at its Test Area North Facility. To determine feasibility, microcosm and bioreactor studies were conducted to ascertain the ability of indigenous microbes to convert trichloroethene and dichloroethene to non-hazardous byproducts under aerobic and anaerobic conditions, and to measure the kinetics of microbial reactions associated with the degradation process. Microcosms were established from core samples and groundwater obtained from within the contaminant plume. These microcosms were amended with nutrients, under aerobic and anaerobic conditions, to identify electron donors capable of stimulating the degradation process. Results of the anaerobic microcosm studies showed that lactate, acetate and propionate amendments stimulated indigenous cell growth and functioned as effective substrates for reductive degradation of chloroethenes. Bioreactors inoculated with cultures from these anaerobic microcosms were operated under a batch mode for 42 days then converted to a fed-batch mode and operated at a 53-day hydraulic residence time. It was demonstrated that indigenous microbes capable of complete anaerobic reductive dechlorination are present in the subject well. It was also demonstrated that aerobic microbes capable of oxidizing chlorinated compounds produced by anaerobic reductive dechlorination are present. Kinetic data suggest that controlling the type and concentration of electron donors can increase trichlorethene conversion rates. In the event that complete mineralization of trichlorethene does not occur following stimulation, and anaerobic/aerobic treatment scheme is feasible.},
doi = {10.2172/911020},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {11}
}

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