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Title: Charge-associated effects of fullerene derivatives on microbialstructural integrity and central metabolism

Abstract

The effects of four types of fullerene compounds (C60,C60-OH, C60-COOH, C60-NH2) were examined on two model microorganisms(Escherichia coli W3110 and Shewanella oneidensis MR-1). Positivelycharged C60-NH2 at concentrations as low as 10 mg/L inhibited growth andreduced substrate uptake for both microorganisms. Scanning ElectronMicroscopy (SEM) revealed damage to cellular structures.Neutrally-charged C60 and C60-OH had mild negative effects on S.oneidensis MR-1, whereas the negatively-charged C60-COOH did not affecteither microorganism s growth. The effect of fullerene compounds onglobal metabolism was further investigated using [3-13C]L-lactateisotopic labeling, which tracks perturbations to metabolic reaction ratesin bacteria by examining the change in the isotopic labeling pattern inthe resulting metabolites (often amino acids).1-3 The 13C isotopomeranalysis from all fullerene-exposed cultures revealed no significantdifferences in isotopomer distributions from unstressed cells. Thisresult indicates that microbial central metabolism is robust toenvironmental stress inflicted by fullerene nanoparticles. In addition,although C60-NH2 compounds caused mechanical stress on the cell wall ormembrane, both S. oneidensis MR-1 and E. coli W3110 can efficientlyalleviate such stress by cell aggregation and precipitation of the toxicnanoparticles. The results presented here favor the hypothesis thatfullerenes cause more membrane stress4, 5, 6 than perturbation to energymetabolism7

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
Sponsoring Org.:
USDOE Director. Office of Science. Office of AdvancedScientific Computing Research. Office of Biological and EnvironmentalResearch; National Institutes of Health Grant R21CA95393-01, USDepartment of Defense Grant BC045345, Defense Advanced Research ProjectsAgency Grant F1ATA05252M001
OSTI Identifier:
910593
Report Number(s):
LBNL-62349
R&D Project: GTL2KK; BnR: KP1102010; TRN: US200724%%266
DOE Contract Number:
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Nano Letters; Journal Volume: 7; Journal Issue: 3; Related Information: Journal Publication Date: 2007
Country of Publication:
United States
Language:
English
Subject:
54; BACTERIA; CELL WALL; FULLERENES; HYPOTHESIS; MEMBRANES; METABOLISM; METABOLITES; MICROORGANISMS; PRECIPITATION; REACTION KINETICS; SCANNING ELECTRON MICROSCOPY; SUBSTRATES; fullerene C60-serinol C60 C60-amine C60-carboxylatenanoparticle nanotoxicity toxicity flux isotopomer distribution aminoacids Ecoli Shewanella oneidensisMR-1 SEM Scanning ElectronMicroscopy

Citation Formats

Tang, Yinjie J., Ashcroft, Jared M., Chen, Ding, Min, Guangwei, Kim, Chul, Murkhejee, Bipasha, Larabell, Carolyn, Keasling, Jay D., and Chen,Fanqing Frank. Charge-associated effects of fullerene derivatives on microbialstructural integrity and central metabolism. United States: N. p., 2007. Web. doi:10.1021/nl063020t.
Tang, Yinjie J., Ashcroft, Jared M., Chen, Ding, Min, Guangwei, Kim, Chul, Murkhejee, Bipasha, Larabell, Carolyn, Keasling, Jay D., & Chen,Fanqing Frank. Charge-associated effects of fullerene derivatives on microbialstructural integrity and central metabolism. United States. doi:10.1021/nl063020t.
Tang, Yinjie J., Ashcroft, Jared M., Chen, Ding, Min, Guangwei, Kim, Chul, Murkhejee, Bipasha, Larabell, Carolyn, Keasling, Jay D., and Chen,Fanqing Frank. Tue . "Charge-associated effects of fullerene derivatives on microbialstructural integrity and central metabolism". United States. doi:10.1021/nl063020t.
@article{osti_910593,
title = {Charge-associated effects of fullerene derivatives on microbialstructural integrity and central metabolism},
author = {Tang, Yinjie J. and Ashcroft, Jared M. and Chen, Ding and Min, Guangwei and Kim, Chul and Murkhejee, Bipasha and Larabell, Carolyn and Keasling, Jay D. and Chen,Fanqing Frank},
abstractNote = {The effects of four types of fullerene compounds (C60,C60-OH, C60-COOH, C60-NH2) were examined on two model microorganisms(Escherichia coli W3110 and Shewanella oneidensis MR-1). Positivelycharged C60-NH2 at concentrations as low as 10 mg/L inhibited growth andreduced substrate uptake for both microorganisms. Scanning ElectronMicroscopy (SEM) revealed damage to cellular structures.Neutrally-charged C60 and C60-OH had mild negative effects on S.oneidensis MR-1, whereas the negatively-charged C60-COOH did not affecteither microorganism s growth. The effect of fullerene compounds onglobal metabolism was further investigated using [3-13C]L-lactateisotopic labeling, which tracks perturbations to metabolic reaction ratesin bacteria by examining the change in the isotopic labeling pattern inthe resulting metabolites (often amino acids).1-3 The 13C isotopomeranalysis from all fullerene-exposed cultures revealed no significantdifferences in isotopomer distributions from unstressed cells. Thisresult indicates that microbial central metabolism is robust toenvironmental stress inflicted by fullerene nanoparticles. In addition,although C60-NH2 compounds caused mechanical stress on the cell wall ormembrane, both S. oneidensis MR-1 and E. coli W3110 can efficientlyalleviate such stress by cell aggregation and precipitation of the toxicnanoparticles. The results presented here favor the hypothesis thatfullerenes cause more membrane stress4, 5, 6 than perturbation to energymetabolism7},
doi = {10.1021/nl063020t},
journal = {Nano Letters},
number = 3,
volume = 7,
place = {United States},
year = {Tue Jan 23 00:00:00 EST 2007},
month = {Tue Jan 23 00:00:00 EST 2007}
}