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Title: Zymomonas pentose-sugar fermenting strains and uses thereof

Abstract

Disclosed in the present invention is a Zymomonas integrant and derivatives of these integrants that posses the ability to ferment pentose into ethanol. The genetic sequences encoding for the pentose-fermenting enzymes are integrated into the Zymomonas in a two-integration event of homologous recombination and transposition. Each operon includes more than one pentose-reducing enzyme encoding sequence. The integrant in some embodiments includes enzyme sequences encoding xylose isomerase, xylulokinase, transketolase and transketolase. The Zymomonas integrants are highly stable, and retain activity for producing the pentose-fermenting enzyme for between 80 to 160 generations. The integrants are also resistant to acetate inhibition, as the integrants demonstrate efficient ethanol production even in the presence of 8 up to 16 grams acetate per liter media. These stably integrated sequences provide a unique Zymomonas that may then be used for the efficient conversion of pentose sugars (xylose, arabinose) to ethanol. Method of using the Zymomonas integrants and derivatives thereof in production of ethanol from cellulosic feedstock is also disclosed. The invention also provides a method for preparing a Zymomonas integrant as part of the present invention. The host Zymomonas strain found particularly useful in the creation of these compositions and methods is Zymomonas mobilis 31821.

Inventors:
 [1];  [2];  [2];  [2];  [3];  [4]
  1. Lakewood, CO
  2. Golden, CO
  3. Littleton, CO
  4. Northglenn, CO
Publication Date:
Research Org.:
Midwest Research Institute (Kansas City, MO)
Sponsoring Org.:
USDOE
OSTI Identifier:
909424
Patent Number(s):
7,223,575
Assignee:
Midwest Research Institute (Kansas City, MO) GFO
DOE Contract Number:
AC36-99GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Zhang, Min, Chou, Yat-Chen, Howe, William, Eddy, Christine, Evans, Kent, and Mohagheghi, Ali. Zymomonas pentose-sugar fermenting strains and uses thereof. United States: N. p., 2007. Web.
Zhang, Min, Chou, Yat-Chen, Howe, William, Eddy, Christine, Evans, Kent, & Mohagheghi, Ali. Zymomonas pentose-sugar fermenting strains and uses thereof. United States.
Zhang, Min, Chou, Yat-Chen, Howe, William, Eddy, Christine, Evans, Kent, and Mohagheghi, Ali. Tue . "Zymomonas pentose-sugar fermenting strains and uses thereof". United States. doi:. https://www.osti.gov/servlets/purl/909424.
@article{osti_909424,
title = {Zymomonas pentose-sugar fermenting strains and uses thereof},
author = {Zhang, Min and Chou, Yat-Chen and Howe, William and Eddy, Christine and Evans, Kent and Mohagheghi, Ali},
abstractNote = {Disclosed in the present invention is a Zymomonas integrant and derivatives of these integrants that posses the ability to ferment pentose into ethanol. The genetic sequences encoding for the pentose-fermenting enzymes are integrated into the Zymomonas in a two-integration event of homologous recombination and transposition. Each operon includes more than one pentose-reducing enzyme encoding sequence. The integrant in some embodiments includes enzyme sequences encoding xylose isomerase, xylulokinase, transketolase and transketolase. The Zymomonas integrants are highly stable, and retain activity for producing the pentose-fermenting enzyme for between 80 to 160 generations. The integrants are also resistant to acetate inhibition, as the integrants demonstrate efficient ethanol production even in the presence of 8 up to 16 grams acetate per liter media. These stably integrated sequences provide a unique Zymomonas that may then be used for the efficient conversion of pentose sugars (xylose, arabinose) to ethanol. Method of using the Zymomonas integrants and derivatives thereof in production of ethanol from cellulosic feedstock is also disclosed. The invention also provides a method for preparing a Zymomonas integrant as part of the present invention. The host Zymomonas strain found particularly useful in the creation of these compositions and methods is Zymomonas mobilis 31821.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 29 00:00:00 EDT 2007},
month = {Tue May 29 00:00:00 EDT 2007}
}

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