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Glycoprotein synthesis

Patent ·
OSTI ID:909397
 [1];  [2];  [2]
  1. La Jolla, CA
  2. San Diego, CA
+ Show Author Affiliations
Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.
Research Organization:
The Scripps Research Institute (La Jolla, CA)
Sponsoring Organization:
United States Department of Energy
DOE Contract Number:
FG03-00ER45812
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
7,217,809
Application Number:
11/094,677
OSTI ID:
909397
Country of Publication:
United States
Language:
English

References (42)

Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli journal March 2001
Cofactor Biosynthesis: A Mechanistic Perspective book December 1997
Purification and properties of β-mannosyltransferase that synthesizes Man-β-GlcNAc-GlcNAc-pyrophosphoryl-dolichol journal October 1986
Stereoselective phase transfer catalyzed syntheses of glycosyloxysuccinimides and their transformations into glycoprobes journal June 1995
[18] Glycosyltransferases in glycobiology book January 1994
A novel method for the specific glycosylation of proteins journal November 1991
Novel cofactors via post-translational modifications of enzyme active sites journal July 2000
A general approach for the generation of orthogonal tRNAs journal September 2001
Exploring the Limits of Codon and Anticodon Size journal February 2002
Unnatural amino acids as probes of protein structure and function journal December 2000
Chemoselective Approaches to Glycoprotein Assembly journal September 2001
Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine journal March 1992
A New Strategy for the Site-Specific Modification of Proteins in Vivo journal June 2003
Synthesis of Glycoproteins journal February 2002
Intein-Mediated Synthesis of Proteins Containing Carbohydrates and Other Molecular Probes journal June 2000
A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins journal May 2000
Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli journal March 2002
Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
  • Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
  • Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027 https://doi.org/10.1021/ja027007w
journal July 2002
Dynamic Glycosylation of the Transcription Factor CREB: A Potential Role in Gene Regulation journal June 2003
Site-Specific Protein Modification Using a Ketone Handle
  • Cornish, Virginia W.; Hahn, Klaus M.; Schultz, Peter G.
  • Journal of the American Chemical Society, Vol. 118, Issue 34, p. 8150-8151 https://doi.org/10.1021/ja961216x
journal January 1996
Enzymatic Glycoprotein Synthesis:  Preparation of Ribonuclease Glycoforms via Enzymatic Glycopeptide Condensation and Glycosylation journal March 1997
Structurally Modified Firefly Luciferase. Effects of Amino Acid Substitution at Position 286
  • Arslan, Tuncer; Mamaev, Sergey V.; Mamaeva, Natalia V.
  • Journal of the American Chemical Society, Vol. 119, Issue 45, p. 10877-10887 https://doi.org/10.1021/ja971927a
journal November 1997
Solution- and Solid-Phase Synthesis of N-Protected Glycopeptide Esters of the Benzyl Type as Substrates for Subtilisin-Catalyzed Glycopeptide Couplings journal March 1998
Fmoc-Based Synthesis of Peptide- α Thioesters:  Application to the Total Chemical Synthesis of a Glycoprotein by Native Chemical Ligation journal December 1999
Aminooxy-, Hydrazide-, and Thiosemicarbazide-Functionalized Saccharides:  Versatile Reagents for Glycoconjugate Synthesis journal October 1998
Solid-Phase Synthesis of Thioether-Linked Glycopeptide Mimics for Application to Glycoprotein Semisynthesis journal May 2002
An efficient system for the evolution of aminoacyl-tRNA synthetase specificity journal September 2002
Addition of the keto functional group to the genetic code of Escherichia coli journal December 2002
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli journal August 2002
Expressed protein ligation: A general method for protein engineering journal June 1998
Progress toward the evolution of an organism with an expanded genetic code journal April 1999
Biological roles of oligosaccharides: all of the theories are correct journal April 1993
Glycosylation of Nucleocytoplasmic Proteins: Signal Transduction and O-GlcNAc journal March 2001
Toward Automated Synthesis of Oligosaccharides and Glycoproteins journal March 2001
Chemical Glycobiology journal March 2001
Expanding the Genetic Code of Escherichia coli journal April 2001
An Expanded Eukaryotic Genetic Code journal August 2003
Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis journal May 1997
N-Linked Glycosylation in Campylobacter jejuni and Its Functional Transfer into E. coli journal November 2002
Biodegradation of Aromatic Compounds by Escherichia coli journal December 2001
Recent Progress in the Solid-phase Synthesis of Glycopeptide journal July 2000
Structure of Tyrosyl-T/RNA Synthetase Refined at 2.3 Angstroms Resolution Interaction of the Enzyme with the Tyrosyladenylate Intermediate report October 1989

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