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Title: Nanovehicles based Bioassay Labels

Abstract

In this article, we review recent advances of our group in nanoparticle labels based bioassay. Apoferritin and silica nanoparticles have been used as nanovehicles to load large amount of markers for highly sensitive bioassay. Markers loaded apoferritin, apoferritin-templated metallic phosphate nanoparticles, and poly [guanine] coated silica nanoparticles have been prepared, characterized and used as labels for highly sensitive bioassay of protein and DNA. Dissociation and reconstitution characteristics at different pH as well as the special cavity structure of apoferritin nanovehicle provides a simple and convenient route to prepare versatile nanoparticle labels and avoid the complicated and tedious synthesis process of conventional nanoparticle labels. The optical and electrochemical characteristics of the prepared nanoparticle labels are easily controlled by loading different optical or electrochemical markers. Additionally, the use of apoferritin nanovehicle as template for synthesis of metallic phosphate nanoparticle labels offers fast route to prepare uniform-size metallic nanoparticle labels for electrochemical bioassay and avoids the traditional harsh dissolution conditions to dissolve metallic nanoparticle tags (that is, the strong-acid dissolution of quantum dots and gold nanoparticles) during the stripping analysis step. Silica nanoparticle has also been used as nanovehicle to carry thousands of poly [guanine] tracers, which was used to enhance the oxidationmore » current of Ru(bpy)32+, resulting in enhanced sensitivity of electrochemical immunoassay. The new nanovehicle-based labels have been used for highly sensitive electrochemical detection of DNA and protein biomarkers, such as tumor necrosis factor-alpha (TNF-a). The high sensitivity and selectivity make these labels a useful addition to the armory of nanoparticle-based bioassay. The new nanovehicles based labels hold great promise for multiplex protein and DNA detection and for enhancing the sensitivity of other bioassays.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
908725
Report Number(s):
PNNL-SA-53315
17505; 12490; 6899; 400412000; TRN: US200722%%885
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Electroanalysis, 19(7-8):777-785; Journal Volume: 19; Journal Issue: 7-8
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BIOASSAY; DISSOCIATION; DISSOLUTION; DNA; GOLD; GUANINE; IMMUNOASSAY; NECROSIS; OXIDATION; PHOSPHATES; PROTEINS; QUANTUM DOTS; SENSITIVITY; SILICA; SYNTHESIS; Nanoparticles, Nanovehicles, Immunoassays; Environmental Molecular Sciences Laboratory

Citation Formats

Liu, Guodong, Wang, Jun, Wu, Hong, Lin, Ying-Ying, and Lin, Yuehe. Nanovehicles based Bioassay Labels. United States: N. p., 2007. Web. doi:10.1002/elan.200603787.
Liu, Guodong, Wang, Jun, Wu, Hong, Lin, Ying-Ying, & Lin, Yuehe. Nanovehicles based Bioassay Labels. United States. doi:10.1002/elan.200603787.
Liu, Guodong, Wang, Jun, Wu, Hong, Lin, Ying-Ying, and Lin, Yuehe. Sun . "Nanovehicles based Bioassay Labels". United States. doi:10.1002/elan.200603787.
@article{osti_908725,
title = {Nanovehicles based Bioassay Labels},
author = {Liu, Guodong and Wang, Jun and Wu, Hong and Lin, Ying-Ying and Lin, Yuehe},
abstractNote = {In this article, we review recent advances of our group in nanoparticle labels based bioassay. Apoferritin and silica nanoparticles have been used as nanovehicles to load large amount of markers for highly sensitive bioassay. Markers loaded apoferritin, apoferritin-templated metallic phosphate nanoparticles, and poly [guanine] coated silica nanoparticles have been prepared, characterized and used as labels for highly sensitive bioassay of protein and DNA. Dissociation and reconstitution characteristics at different pH as well as the special cavity structure of apoferritin nanovehicle provides a simple and convenient route to prepare versatile nanoparticle labels and avoid the complicated and tedious synthesis process of conventional nanoparticle labels. The optical and electrochemical characteristics of the prepared nanoparticle labels are easily controlled by loading different optical or electrochemical markers. Additionally, the use of apoferritin nanovehicle as template for synthesis of metallic phosphate nanoparticle labels offers fast route to prepare uniform-size metallic nanoparticle labels for electrochemical bioassay and avoids the traditional harsh dissolution conditions to dissolve metallic nanoparticle tags (that is, the strong-acid dissolution of quantum dots and gold nanoparticles) during the stripping analysis step. Silica nanoparticle has also been used as nanovehicle to carry thousands of poly [guanine] tracers, which was used to enhance the oxidation current of Ru(bpy)32+, resulting in enhanced sensitivity of electrochemical immunoassay. The new nanovehicle-based labels have been used for highly sensitive electrochemical detection of DNA and protein biomarkers, such as tumor necrosis factor-alpha (TNF-a). The high sensitivity and selectivity make these labels a useful addition to the armory of nanoparticle-based bioassay. The new nanovehicles based labels hold great promise for multiplex protein and DNA detection and for enhancing the sensitivity of other bioassays.},
doi = {10.1002/elan.200603787},
journal = {Electroanalysis, 19(7-8):777-785},
number = 7-8,
volume = 19,
place = {United States},
year = {Sun Apr 01 00:00:00 EDT 2007},
month = {Sun Apr 01 00:00:00 EDT 2007}
}