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Title: Isolation and Characterization of Four Gram-Positive Nickel-Tolerant Microorganisms from Contaminated Sediments

Abstract

Microbial communities from riparian sediments contaminated with high levels of Ni and U were examined for metal-tolerant microorganisms. Isolation of four aerobic Ni-tolerant, Gram-positive heterotrophic bacteria indicated selection pressure from Ni. These isolates were identified as Arthrobacter oxydans NR-1, Streptomyces galbus NR-2, Streptomyces aureofaciens NR-3, and Kitasatospora cystarginea NR-4 based on partial 16S rDNA sequences. A functional gene microarray containing gene probes for functions associated with biogeochemical cycling, metal homeostasis, and organic contaminant degradation showed little overlap among the four isolates. Fifteen of the genes were detected in all four isolates with only two of these related to metal resistance, specifically to tellurium. Each of the four isolates also displayed resistance to at least one of six antibiotics tested, with resistance to kanamycin, gentamycin, and ciprofloxacin observed in at least two of the isolates. Further characterization of S. aureofaciens NR-3 and K. cystarginea NR-4 demonstrated that both isolates expressed Ni tolerance constitutively. In addition, both were able to grow in higher concentrations of Ni at pH 6 as compared with pH 7 (42.6 and 8.5 mM Ni at pH 6 and 7, respectively). Tolerance to Cd, Co, and Zn was also examined in these two isolates; a similar pH-dependent metalmore » tolerance was observed when grown with Co and Zn. Neither isolate was tolerant to Cd. These findings suggest that Ni is exerting a selection pressure at this site for metal-resistant actinomycetes.« less

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Savannah River Ecology Laboratory (SREL), Aiken, SC
Sponsoring Org.:
USDOE
OSTI Identifier:
908664
Report Number(s):
SREL-3033
Journal ID: ISSN 0095-3628; MCBEBU; TRN: US200722%%851
DOE Contract Number:
DE-FC09-07SR22506
Resource Type:
Journal Article
Resource Relation:
Journal Name: Microbial Ecology; Journal Volume: 53
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ANTIBIOTICS; BACTERIA; COMMUNITIES; FUNCTIONALS; GENES; HOMEOSTASIS; MICROORGANISMS; PROBES; SEDIMENTS; STREPTOMYCES; TELLURIUM; TOLERANCE

Citation Formats

Van Nostrand, J. D., Khijniak, T. V., Gentry, T. J., Novak, M. T., Sowder, A. G., Zhou, J. Z., Bertsch, P. M., and Morris, P. J.. Isolation and Characterization of Four Gram-Positive Nickel-Tolerant Microorganisms from Contaminated Sediments. United States: N. p., 2007. Web. doi:10.1007/s00248-006-9160-7.
Van Nostrand, J. D., Khijniak, T. V., Gentry, T. J., Novak, M. T., Sowder, A. G., Zhou, J. Z., Bertsch, P. M., & Morris, P. J.. Isolation and Characterization of Four Gram-Positive Nickel-Tolerant Microorganisms from Contaminated Sediments. United States. doi:10.1007/s00248-006-9160-7.
Van Nostrand, J. D., Khijniak, T. V., Gentry, T. J., Novak, M. T., Sowder, A. G., Zhou, J. Z., Bertsch, P. M., and Morris, P. J.. Mon . "Isolation and Characterization of Four Gram-Positive Nickel-Tolerant Microorganisms from Contaminated Sediments". United States. doi:10.1007/s00248-006-9160-7.
@article{osti_908664,
title = {Isolation and Characterization of Four Gram-Positive Nickel-Tolerant Microorganisms from Contaminated Sediments},
author = {Van Nostrand, J. D. and Khijniak, T. V. and Gentry, T. J. and Novak, M. T. and Sowder, A. G. and Zhou, J. Z. and Bertsch, P. M. and Morris, P. J.},
abstractNote = {Microbial communities from riparian sediments contaminated with high levels of Ni and U were examined for metal-tolerant microorganisms. Isolation of four aerobic Ni-tolerant, Gram-positive heterotrophic bacteria indicated selection pressure from Ni. These isolates were identified as Arthrobacter oxydans NR-1, Streptomyces galbus NR-2, Streptomyces aureofaciens NR-3, and Kitasatospora cystarginea NR-4 based on partial 16S rDNA sequences. A functional gene microarray containing gene probes for functions associated with biogeochemical cycling, metal homeostasis, and organic contaminant degradation showed little overlap among the four isolates. Fifteen of the genes were detected in all four isolates with only two of these related to metal resistance, specifically to tellurium. Each of the four isolates also displayed resistance to at least one of six antibiotics tested, with resistance to kanamycin, gentamycin, and ciprofloxacin observed in at least two of the isolates. Further characterization of S. aureofaciens NR-3 and K. cystarginea NR-4 demonstrated that both isolates expressed Ni tolerance constitutively. In addition, both were able to grow in higher concentrations of Ni at pH 6 as compared with pH 7 (42.6 and 8.5 mM Ni at pH 6 and 7, respectively). Tolerance to Cd, Co, and Zn was also examined in these two isolates; a similar pH-dependent metal tolerance was observed when grown with Co and Zn. Neither isolate was tolerant to Cd. These findings suggest that Ni is exerting a selection pressure at this site for metal-resistant actinomycetes.},
doi = {10.1007/s00248-006-9160-7},
journal = {Microbial Ecology},
number = ,
volume = 53,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}