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Cast-to-shape electrokinetic trapping medium

Patent ·
OSTI ID:908414
 [1];  [2];  [3];  [4]
  1. Livermore, CA
  2. Rolla, MO
  3. Golden, CO
  4. Pleasanton, CA
+ Show Author Affiliations
A three-dimensional microporous polymer network material, or monolith, cast-to-shape in a microchannel. The polymer monolith, produced by a phase separation process, is capable of trapping and retaining charged protein species from a mixture of charged and uncharged species under the influence of an applied electric field. The retained charged protein species are released from the porous polymer monolith by a pressure driven flow in the substantial absence of the electric field. The pressure driven flow is independent of direction and thus neither means to reverse fluid flow nor a multi-directional flow field is required, a single flow through the porous polymer monolith can be employed, in contrast to prior art systems. The monolithic polymer material produced by the invention can function as a chromatographic medium. Moreover, by virtue of its ability to retain charged protein species and quantitatively release the retained species the porous polymer monolith can serve as a means for concentrating charged protein species from, for example, a dilute solution.
Research Organization:
Sandia National Laboratories (SNL-CA), Livermore, CA
Sponsoring Organization:
United States Department of Energy
DOE Contract Number:
AC04-94AL85000;
Assignee:
Sandia National Laboratories (Livermore, CA)
Patent Number(s):
7,052,608
OSTI ID:
908414
Country of Publication:
United States
Language:
English

References (3)

Photopolymerized Sol−Gel Monoliths for Capillary Electrochromatography journal August 2001
Strategy for On-Line Preconcentration in Chromatographic Separations journal November 2001
On-Line Preconcentration in Capillary Electrochromatography Using a Porous Monolith Together with Solvent Gradient and Sample Stacking journal November 2001

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36 MATERIALS SCIENCE