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Title: Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain

Abstract

A novel sulfate-reducing bacterium (strain DePue) closelyrelated to Desulfovibrio vulgaris ssp. vulgaris strain Hildenborough wasisolated from the sediment of a heavy-metal impacted lake usingestablished techniques. Although few physiological differences betweenstrains DePue and Hildenborough were observed, pulsed-field gelelectrophoresis (PFGE) revealed a significant genome reduction in strainDePue. Comparative whole-genome microarray and PCR analyses demonstratedthat the absence of genes annotated in the Hildenborough genome as phageor phage-related contributed to the significant genome reduction instrain DePue. Two morphotypically distinct temperate bacteriophage fromstrain Hildenborough were recovered using strain DePue as a host forplaque isolation.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
COLLABORATION - U. ofWashington
OSTI Identifier:
903368
Report Number(s):
LBNL-60457
Journal ID: ISSN 1462-2912; R&D Project: VGTLTH; BnR: KP1102010; TRN: US200720%%300
DOE Contract Number:
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental Microbiology; Journal Volume: 8; Journal Issue: 10; Related Information: Journal Publication Date: November,2006
Country of Publication:
United States
Language:
English
Subject:
59; 54; BACTERIOPHAGES; DESULFOVIBRIO; ELECTROPHORESIS; GENES; SEDIMENTS; STRAINS; bioremediation comparative genomics environmental genomicssulfate reducers

Citation Formats

Walker, C.B., Stolyar, S.S., Pinel, N., Yen, H.C., He, Z., Zhou,J., Wall, J.D., and Stahl, D.A. Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain. United States: N. p., 2007. Web.
Walker, C.B., Stolyar, S.S., Pinel, N., Yen, H.C., He, Z., Zhou,J., Wall, J.D., & Stahl, D.A. Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain. United States.
Walker, C.B., Stolyar, S.S., Pinel, N., Yen, H.C., He, Z., Zhou,J., Wall, J.D., and Stahl, D.A. Mon . "Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain". United States. doi:.
@article{osti_903368,
title = {Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain},
author = {Walker, C.B. and Stolyar, S.S. and Pinel, N. and Yen, H.C. and He, Z. and Zhou,J. and Wall, J.D. and Stahl, D.A.},
abstractNote = {A novel sulfate-reducing bacterium (strain DePue) closelyrelated to Desulfovibrio vulgaris ssp. vulgaris strain Hildenborough wasisolated from the sediment of a heavy-metal impacted lake usingestablished techniques. Although few physiological differences betweenstrains DePue and Hildenborough were observed, pulsed-field gelelectrophoresis (PFGE) revealed a significant genome reduction in strainDePue. Comparative whole-genome microarray and PCR analyses demonstratedthat the absence of genes annotated in the Hildenborough genome as phageor phage-related contributed to the significant genome reduction instrain DePue. Two morphotypically distinct temperate bacteriophage fromstrain Hildenborough were recovered using strain DePue as a host forplaque isolation.},
doi = {},
journal = {Environmental Microbiology},
number = 10,
volume = 8,
place = {United States},
year = {Mon Apr 02 00:00:00 EDT 2007},
month = {Mon Apr 02 00:00:00 EDT 2007}
}