Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain

Title: Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain

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Abstract

A novel sulfate-reducing bacterium (strain DePue) closelyrelated to Desulfovibrio vulgaris ssp. vulgaris strain Hildenborough wasisolated from the sediment of a heavy-metal impacted lake usingestablished techniques. Although few physiological differences betweenstrains DePue and Hildenborough were observed, pulsed-field gelelectrophoresis (PFGE) revealed a significant genome reduction in strainDePue. Comparative whole-genome microarray and PCR analyses demonstratedthat the absence of genes annotated in the Hildenborough genome as phageor phage-related contributed to the significant genome reduction instrain DePue. Two morphotypically distinct temperate bacteriophage fromstrain Hildenborough were recovered using strain DePue as a host forplaque isolation.

Authors:: Walker, C.B.; Stolyar, S.S.; Pinel, N.; Yen, H.C.; He, Z.; Zhou,J.; Wall, J.D.; Stahl, D.A.

Publication Date:: Mon Apr 02 00:00:00 EDT 2007

Research Org.:: COLLABORATION - U. ofWashington

OSTI Identifier:: 903368

Report Number(s):: LBNL-60457
Journal ID: ISSN 1462-2912; R&D Project: VGTLTH; BnR: KP1102010; TRN: US200720%%300

DOE Contract Number:: DE-AC02-05CH11231

Resource Type:: Journal Article

Resource Relation:: Journal Name: Environmental Microbiology; Journal Volume: 8; Journal Issue: 10; Related Information: Journal Publication Date: November,2006

Country of Publication:: United States

Language:: English

Subject:: 59; 54; BACTERIOPHAGES; DESULFOVIBRIO; ELECTROPHORESIS; GENES; SEDIMENTS; STRAINS; bioremediation comparative genomics environmental genomicssulfate reducers

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                    Walker, C.B., Stolyar, S.S., Pinel, N., Yen, H.C., He, Z., Zhou,J., Wall, J.D., and Stahl, D.A. Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain.  United States: N. p., 2007. 
        Web.

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                    Walker, C.B., Stolyar, S.S., Pinel, N., Yen, H.C., He, Z., Zhou,J., Wall, J.D., & Stahl, D.A. Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain.  United States.

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                    Walker, C.B., Stolyar, S.S., Pinel, N., Yen, H.C., He, Z., Zhou,J., Wall, J.D., and Stahl, D.A. Mon .  
        "Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain".  United States.  
        doi:.

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@article{osti_903368,

  title        = {Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain},

  author       = {Walker, C.B. and Stolyar, S.S. and Pinel, N. and Yen, H.C. and He, Z. and Zhou,J. and Wall, J.D. and Stahl, D.A.},

  abstractNote = {A novel sulfate-reducing bacterium (strain DePue) closelyrelated to Desulfovibrio vulgaris ssp. vulgaris strain Hildenborough wasisolated from the sediment of a heavy-metal impacted lake usingestablished techniques. Although few physiological differences betweenstrains DePue and Hildenborough were observed, pulsed-field gelelectrophoresis (PFGE) revealed a significant genome reduction in strainDePue. Comparative whole-genome microarray and PCR analyses demonstratedthat the absence of genes annotated in the Hildenborough genome as phageor phage-related contributed to the significant genome reduction instrain DePue. Two morphotypically distinct temperate bacteriophage fromstrain Hildenborough were recovered using strain DePue as a host forplaque isolation.},

  doi          = {},

  journal      = {Environmental Microbiology},

  number       = 10,

  volume       = 8,

  place        = {United States},

  year         = {Mon Apr 02 00:00:00 EDT 2007},

  month        = {Mon Apr 02 00:00:00 EDT 2007}

}

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Recovery of temperate Desulfovibrio vulgaris bacteriophage using a novel host strain

Christopher B.,Walker ; Sergey S.,Stolyar ; Nic,Pinel ; ... - Environmental Microbiology
DOI: 10.1111/j.1462-2920.2006.01075.x
Development of a Markerless Genetic Exchange System in Desulfovibrio vulgaris Hildenborough and Its Use in Generating a Strain with Increased Transformation Efficiency

Keller, Kimberly L. ; Bender, Kelly S. ; Wall, Judy D. - Applied and Environmental Microbiology

In recent years, the genetic manipulation of the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough has seen enormous progress. In spite of this progress, the current marker exchange deletion method does not allow for easy selection of multiple sequential gene deletions in a single strain because of the limited number of selectable markers available in D. vulgaris. To broaden the repertoire of genetic tools for manipulation, an in-frame, markerless deletion system has been developed. The counterselectable marker that makes this deletion system possible is the pyrimidine salvage enzyme, uracil phosphoribosyltransferase, encoded by upp. In wild-type D. vulgaris, growth was shown to bemore »« less
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Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with Desulfovibrio vulgaris Hildenborough and Methanobacterium congolense: Global transcriptomic and proteomic analyses

Men, Y. ; Feil, H. ; VerBerkmoes, N.C. ; ... - The ISME Journal

Dehalococcoides ethenogenes strain 195 (DE195) was grown in a sustainable syntrophic association with Desulfovibrio vulgaris Hildenborough (DVH) as a co-culture, as well as with DVH and the hydrogenotrophic methanogen Methanobacterium congolense (MC) as a tri-culture using lactate as the sole energy and carbon source. In the co- and tri-cultures, maximum dechlorination rates of DE195 were enhanced by approximately three times (11.0±0.01 lmol per day for the co-culture and 10.1±0.3 lmol per day for the tri-culture) compared with DE195 grown alone (3.8±0.1 lmol per day). Cell yield of DE195 was enhanced in the co-culture (9.0±0.5 x 107 cells per lmol Cl{supmore »« less
DOI: 10.1038/ismej.2011.111

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Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with Desulfovibrio vulgaris Hildenborough and Methanobacterium congolense: global transcriptomic and proteomic analyses

Men, Yujie ; Feil, Helene ; Verberkmoes, Nathan C ; ... - The ISME Journal: Multidisciplinary Journal of Microbial Ecology

Dehalococcoides ethenogenes strain 195 (DE195) was grown in a sustainable syntrophic association with Desulfovibrio vulgaris Hildenborough (DVH) as a co-culture, as well as with DVH and the hydrogenotrophic methanogen Methanobacterium congolense (MC) as a tri-culture using lactate as the sole energy and carbon source. In the co- and tri-cultures, maximum dechlorination rates of DE195 were enhanced by approximately three times (11.0 0.01 lmol per day for the co-culture and 10.1 0.3 lmol per day for the tri-culture) compared with DE195 grown alone (3.8 0.1 lmol per day). Cell yield of DE195 was enhanced in the co-culture (9.0 0.5107 cells permore »« less
DOI: 10.1038/ismej.2011.111
Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with Desulfovibrio vulgaris Hildenborough and Methanobacterium congolense: global transcriptomic and proteomic analyses

Men, Yujie ; Feil, Helene ; VerBerkmoes, Nathan C. ; ... - The ISME Journal

Dehalococcoides ethenogenes strain 195 (DE195) was grown in a sustainable syntrophic association with Desulfovibrio vulgaris Hildenborough (DVH) as a co-culture, as well as with DVH and the hydrogenotrophic methanogen Methanobacterium congolense (MC) as a tri-culture using lactate as the sole energy and carbon source. In the co- and tri-cultures, maximum dechlorination rates of DE195 were enhanced by approximately three times (11.0 0.01 lmol per day for the co-culture and 10.1 0.3 lmol per day for the tri-culture) compared with DE195 grown alone (3.8 0.1 lmol per day). Cell yield of DE195 was enhanced in the co-culture (9.0 0.5107 cells permore »« less
DOI: 10.1038/ismej.2011.111