Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor
Abstract
High levels of nitrate are present in groundwater migratingfrom the former waste disposal ponds at the Y-12 National SecurityComplex in Oak Ridge, TN. A field-scale denitrifying fluidized bedreactor (FBR) was designed, constructed, and operated with ethanol as anelectron donor for the removal of nitrate. After inoculation, biofilmsdeveloped on the granular activated carbon particles. Changes in thebacterial community of the FBR were evaluated with clone libraries (n=500partial sequences) of the small-subunit rRNA gene for samples taken overa 4-month start-up period. Early phases of start-up operationwerecharacterized by a period of selection, followed by low diversity andpredominance by Azoarcus-like sequences. Possible explanations were highpH and nutrient limitations. After amelioration of these conditions,diversification increased rapidly, with the appearance of Dechloromonas,Pseudomonas, and Hydrogenophaga sequences. Changes in NO3, SO4, and pHalso likely contributed to shifts in community composition. The detectionof sulfate-reducing-bacteria-like sequences closely related toDesulfovibrio and Desulfuromonas in the FBR have important implicationsfor downstream applications at the field site.
- Authors:
- Publication Date:
- Research Org.:
- COLLABORATION - MiamiU.
- OSTI Identifier:
- 903366
- Report Number(s):
- LBNL-60455
Journal ID: ISSN 0175-7598; AMBIDG; R&D Project: VGTLTH; BnR: KP1102010; TRN: US200720%%298
- DOE Contract Number:
- DE-AC02-05CH11231
- Resource Type:
- Journal Article
- Resource Relation:
- Journal Name: Applied Microbiology and Biotechnology; Journal Volume: 71; Journal Issue: 5; Related Information: Journal Publication Date: August, 2006
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54; 59; ACTIVATED CARBON; BINDING ENERGY; DESULFOVIBRIO; DIVERSIFICATION; ELECTRONS; ETHANOL; FLUIDIZED BED REACTORS; GENES; INOCULATION; NATIONAL SECURITY; NITRATES; NUTRIENTS; PONDS; PSEUDOMONAS; START-UP; VALENCE; WASTE DISPOSAL; Bioremediation Stress Response sulfate reducers fieldstudies
Citation Formats
Hwang, C., Wu, W.-M., Gentry, T.J., Carley, J., Carroll, S.L., Schadt, C., Watson, D., Jardine, P.M., Zhou, J., Hickey, R.F., Criddle,C.S., and Fields, M.W. Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor. United States: N. p., 2007.
Web.
Hwang, C., Wu, W.-M., Gentry, T.J., Carley, J., Carroll, S.L., Schadt, C., Watson, D., Jardine, P.M., Zhou, J., Hickey, R.F., Criddle,C.S., & Fields, M.W. Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor. United States.
Hwang, C., Wu, W.-M., Gentry, T.J., Carley, J., Carroll, S.L., Schadt, C., Watson, D., Jardine, P.M., Zhou, J., Hickey, R.F., Criddle,C.S., and Fields, M.W. Mon .
"Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor". United States.
doi:.
@article{osti_903366,
title = {Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor},
author = {Hwang, C. and Wu, W.-M. and Gentry, T.J. and Carley, J. and Carroll, S.L. and Schadt, C. and Watson, D. and Jardine, P.M. and Zhou, J. and Hickey, R.F. and Criddle,C.S. and Fields, M.W.},
abstractNote = {High levels of nitrate are present in groundwater migratingfrom the former waste disposal ponds at the Y-12 National SecurityComplex in Oak Ridge, TN. A field-scale denitrifying fluidized bedreactor (FBR) was designed, constructed, and operated with ethanol as anelectron donor for the removal of nitrate. After inoculation, biofilmsdeveloped on the granular activated carbon particles. Changes in thebacterial community of the FBR were evaluated with clone libraries (n=500partial sequences) of the small-subunit rRNA gene for samples taken overa 4-month start-up period. Early phases of start-up operationwerecharacterized by a period of selection, followed by low diversity andpredominance by Azoarcus-like sequences. Possible explanations were highpH and nutrient limitations. After amelioration of these conditions,diversification increased rapidly, with the appearance of Dechloromonas,Pseudomonas, and Hydrogenophaga sequences. Changes in NO3, SO4, and pHalso likely contributed to shifts in community composition. The detectionof sulfate-reducing-bacteria-like sequences closely related toDesulfovibrio and Desulfuromonas in the FBR have important implicationsfor downstream applications at the field site.},
doi = {},
journal = {Applied Microbiology and Biotechnology},
number = 5,
volume = 71,
place = {United States},
year = {Mon Apr 02 00:00:00 EDT 2007},
month = {Mon Apr 02 00:00:00 EDT 2007}
}
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High levels of nitrate are present in groundwater migrating from the former waste disposal ponds at the Y-12 National Security Complex in Oak Ridge, TN. A field-scale denitrifying fluidized bed reactor (FBR) was designed, constructed, and operated with ethanol as an electron donor for the removal of nitrate. After inoculation, biofilms developed on the granular activated carbon particles. Changes in the bacterial community of the FBR were evaluated with clone libraries (n=500 partial sequences) of the small-subunit rRNA gene for samples taken over a 4-month start-up period. Early phases of start-up operation were characterized by a period of selection, followedmore »
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Changes in microbial community structure correlate with stressed operating conditions during start-up of a field-scale denitrifying fluidized bed reactor
High levels of nitrate are present in groundwater migrating from the former waste disposal ponds at the Y-12 National Security Complex in Oak Ridge, TN. A field-scale denitrifying fluidized bed reactor (FBR) was designed, constructed, and operated with ethanol as an electron donor for the removal of nitrate. After inoculation, biofilms developed on the granular activated carbon particles. Changes in the bacterial community of the FBR were evaluated with clone libraries (n=500 partial sequences) of the small-subunit rRNA gene for samples taken over a 4-month start-up period. Early phases of start-up operation were characterized by a period of selection, followedmore » -
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The species (cytospecies) structure and seasonal dynamics of a thermophilic bluegreen algal/bacterial community from Mimbres, New Mexico are described. Using stereological techniques, several common quantitative ecological parameters (biomass, density, distribution measurements, Species Diversity Indices, importance values) were described for the community growing ca. 1 m beneath the water surface on the inner walls of a concrete cistern. Temperature and pH were relatively constant while water flow rates, duration and intensity of light showed seasonal variation. The total volume of the mat occupied by living organsisms did not exceed 2% during the entire year. The remaining volume was occupied by gasmore » -
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Changes in Bacterial And Archaeal Community Structure And Functional Diversity Along a Geochemically Variable Soil Profile
Spatial heterogeneity in physical, chemical, and biological properties of soils allows for the proliferation of diverse microbial communities. Factors influencing the structuring of microbial communities, including availability of nutrients and water, pH, and soil texture, can vary considerably with soil depth and within soil aggregates. Here we investigated changes in the microbial and functional communities within soil aggregates obtained along a soil profile spanning the surface, vadose zone, and saturated soil environments. The composition and diversity of microbial communities and specific functional groups involved in key pathways in the geochemical cycling of nitrogen, Fe, and sulfur were characterized using amore »