Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor

Title: Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor

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Abstract

High levels of nitrate are present in groundwater migratingfrom the former waste disposal ponds at the Y-12 National SecurityComplex in Oak Ridge, TN. A field-scale denitrifying fluidized bedreactor (FBR) was designed, constructed, and operated with ethanol as anelectron donor for the removal of nitrate. After inoculation, biofilmsdeveloped on the granular activated carbon particles. Changes in thebacterial community of the FBR were evaluated with clone libraries (n=500partial sequences) of the small-subunit rRNA gene for samples taken overa 4-month start-up period. Early phases of start-up operationwerecharacterized by a period of selection, followed by low diversity andpredominance by Azoarcus-like sequences. Possible explanations were highpH and nutrient limitations. After amelioration of these conditions,diversification increased rapidly, with the appearance of Dechloromonas,Pseudomonas, and Hydrogenophaga sequences. Changes in NO3, SO4, and pHalso likely contributed to shifts in community composition. The detectionof sulfate-reducing-bacteria-like sequences closely related toDesulfovibrio and Desulfuromonas in the FBR have important implicationsfor downstream applications at the field site.

Authors:: Hwang, C.; Wu, W.-M.; Gentry, T.J.; Carley, J.; Carroll, S.L.; Schadt, C.; Watson, D.; Jardine, P.M.; Zhou, J.; Hickey, R.F.; Criddle,C.S.; Fields, M.W.

Publication Date:: Mon Apr 02 00:00:00 EDT 2007

Research Org.:: COLLABORATION - MiamiU.

OSTI Identifier:: 903366

Report Number(s):: LBNL-60455
Journal ID: ISSN 0175-7598; AMBIDG; R&D Project: VGTLTH; BnR: KP1102010; TRN: US200720%%298

DOE Contract Number:: DE-AC02-05CH11231

Resource Type:: Journal Article

Resource Relation:: Journal Name: Applied Microbiology and Biotechnology; Journal Volume: 71; Journal Issue: 5; Related Information: Journal Publication Date: August, 2006

Country of Publication:: United States

Language:: English

Subject:: 54; 59; ACTIVATED CARBON; BINDING ENERGY; DESULFOVIBRIO; DIVERSIFICATION; ELECTRONS; ETHANOL; FLUIDIZED BED REACTORS; GENES; INOCULATION; NATIONAL SECURITY; NITRATES; NUTRIENTS; PONDS; PSEUDOMONAS; START-UP; VALENCE; WASTE DISPOSAL; Bioremediation Stress Response sulfate reducers fieldstudies

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                    Hwang, C., Wu, W.-M., Gentry, T.J., Carley, J., Carroll, S.L., Schadt, C., Watson, D., Jardine, P.M., Zhou, J., Hickey, R.F., Criddle,C.S., and Fields, M.W.. Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor.  United States: N. p., 2007. 
        Web.

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                    Hwang, C., Wu, W.-M., Gentry, T.J., Carley, J., Carroll, S.L., Schadt, C., Watson, D., Jardine, P.M., Zhou, J., Hickey, R.F., Criddle,C.S., & Fields, M.W.. Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor.  United States.

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                    Hwang, C., Wu, W.-M., Gentry, T.J., Carley, J., Carroll, S.L., Schadt, C., Watson, D., Jardine, P.M., Zhou, J., Hickey, R.F., Criddle,C.S., and Fields, M.W.. Mon .  
        "Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor".  United States.  
        doi:.

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@article{osti_903366,

  title        = {Changes in bacterial community structure correlate with initialoperating conditions of a field-scale denitrifying fluidized bedreactor},

  author       = {Hwang, C. and Wu, W.-M. and Gentry, T.J. and Carley, J. and Carroll, S.L. and Schadt, C. and Watson, D. and Jardine, P.M. and Zhou, J. and Hickey, R.F. and Criddle,C.S. and Fields, M.W.},

  abstractNote = {High levels of nitrate are present in groundwater migratingfrom the former waste disposal ponds at the Y-12 National SecurityComplex in Oak Ridge, TN. A field-scale denitrifying fluidized bedreactor (FBR) was designed, constructed, and operated with ethanol as anelectron donor for the removal of nitrate. After inoculation, biofilmsdeveloped on the granular activated carbon particles. Changes in thebacterial community of the FBR were evaluated with clone libraries (n=500partial sequences) of the small-subunit rRNA gene for samples taken overa 4-month start-up period. Early phases of start-up operationwerecharacterized by a period of selection, followed by low diversity andpredominance by Azoarcus-like sequences. Possible explanations were highpH and nutrient limitations. After amelioration of these conditions,diversification increased rapidly, with the appearance of Dechloromonas,Pseudomonas, and Hydrogenophaga sequences. Changes in NO3, SO4, and pHalso likely contributed to shifts in community composition. The detectionof sulfate-reducing-bacteria-like sequences closely related toDesulfovibrio and Desulfuromonas in the FBR have important implicationsfor downstream applications at the field site.},

  doi          = {},

  journal      = {Applied Microbiology and Biotechnology},

  number       = 5,

  volume       = 71,

  place        = {United States},

  year         = {Mon Apr 02 00:00:00 EDT 2007},

  month        = {Mon Apr 02 00:00:00 EDT 2007}

}

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