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Title: Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change

Abstract

Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.

Inventors:
 [1];  [2];  [3]
  1. Danville, CA
  2. Severna Park, MD
  3. Oakland, CA
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
903351
Patent Number(s):
7,157,232
Application Number:
11/020,731
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Miles, Robin R, Belgrader, Phillip, and Fuller, Christopher D. Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change. United States: N. p., 2007. Web.
Miles, Robin R, Belgrader, Phillip, & Fuller, Christopher D. Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change. United States.
Miles, Robin R, Belgrader, Phillip, and Fuller, Christopher D. Tue . "Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change". United States. https://www.osti.gov/servlets/purl/903351.
@article{osti_903351,
title = {Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change},
author = {Miles, Robin R and Belgrader, Phillip and Fuller, Christopher D},
abstractNote = {Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.},
doi = {},
url = {https://www.osti.gov/biblio/903351}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {1}
}

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