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Title: Nucleotide sequences specific to Francisella tularensis and methods for the detection of Francisella tularensis

Abstract

Described herein is the identification of nucleotide sequences specific to Francisella tularensis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

Inventors:
 [1];  [2];  [3];  [4];  [4];  [4];  [4]
  1. Tracy, CA
  2. San Mateo, CA
  3. Berkeley, CA
  4. Livermore, CA
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); University of California
Sponsoring Org.:
USDOE
OSTI Identifier:
902827
Patent Number(s):
7,172,868
Application Number:
10/630,154
Assignee:
The Regents of the University of California (Oakland, CA) LLNL
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

McCready, Paula M, Radnedge, Lyndsay, Andersen, Gary L, Ott, Linda L, Slezak, Thomas R, Kuczmarski, Thomas A, and Vitalis, Elizabeth A. Nucleotide sequences specific to Francisella tularensis and methods for the detection of Francisella tularensis. United States: N. p., 2007. Web.
McCready, Paula M, Radnedge, Lyndsay, Andersen, Gary L, Ott, Linda L, Slezak, Thomas R, Kuczmarski, Thomas A, & Vitalis, Elizabeth A. Nucleotide sequences specific to Francisella tularensis and methods for the detection of Francisella tularensis. United States.
McCready, Paula M, Radnedge, Lyndsay, Andersen, Gary L, Ott, Linda L, Slezak, Thomas R, Kuczmarski, Thomas A, and Vitalis, Elizabeth A. Tue . "Nucleotide sequences specific to Francisella tularensis and methods for the detection of Francisella tularensis". United States. doi:. https://www.osti.gov/servlets/purl/902827.
@article{osti_902827,
title = {Nucleotide sequences specific to Francisella tularensis and methods for the detection of Francisella tularensis},
author = {McCready, Paula M and Radnedge, Lyndsay and Andersen, Gary L and Ott, Linda L and Slezak, Thomas R and Kuczmarski, Thomas A and Vitalis, Elizabeth A},
abstractNote = {Described herein is the identification of nucleotide sequences specific to Francisella tularensis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 06 00:00:00 EST 2007},
month = {Tue Feb 06 00:00:00 EST 2007}
}

Patent:

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  • Described herein is the identification of nucleotide sequences specific to Francisella tularensis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.
  • Nucleotide sequences specific to Yersinia pestis that serve as markers or signatures for identification of this bacterium were identified. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.
  • Nucleotide sequences specific to Brucella that serves as a marker or signature for identification of this bacterium were identified. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.
  • A method for rapid and efficient detection of a target DNA or RNA sequence is provided. A primer having a 3'-hydroxyl group at one end and having a sequence of nucleotides sufficiently homologous with an identifying sequence of nucleotides in the target DNA is selected. The primer is hybridized to the identifying sequence of nucleotides on the DNA or RNA sequence and a reporter molecule is synthesized on the target sequence by progressively binding complementary nucleotides to the primer, where the complementary nucleotides include nucleotides labeled with a fluorophore. Fluorescence emitted by fluorophores on single reporter molecules is detected tomore » identify the target DNA or RNA sequence.« less
  • To evaluate the sensitivity and specificity of the Idaho Technologies FilmArray® Biothreat Panel for the detection of Bacillus anthracis (Ba), Francisella tularensis (Ft), and Yersinia pestis (Yp) DNA, and demonstrate the detection of Ba spores. Methods and Results: DNA samples from Ba, Ft and Yp strains and near-neighbors, and live Ba spores were analyzed using the Biothreat Panel, a multiplexed PCR-based assay for 17 pathogens and toxins. Sensitivity studies with DNA suggest a limit of detection of 250 genome equivalents (GEs) per sample. Furthermore, the correct call of Ft, Yp or Bacillus species was made in 63 of 72 samplesmore » tested at 25 GE or less. With samples containing 25 Ba Sterne spores, at least one of the two possible Ba markers were identified in all samples tested. We observed no cross-reactivity with near-neighbor DNAs.« less