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Title: Multiplexed Ion Mobility Spectrometry - Orthogonal Time-Of-Flight Mass Spectrometry

Abstract

Ion mobility spectrometry (IMS) coupled to orthogonal time-of-flight mass spectrometry (TOF) has shown significant promise for the characterization of complex biological mixtures. The enormous complexity of biological samples (e.g. from proteomics) and the need for both biological and technical analysis replicates imposes major challenges for multidimensional separation platforms in regard to both sensitivity and sample throughput. A major potential attraction of the IMS-TOF MS platform is separation speeds exceeding that of conventional condensed-phase separations by orders of magnitude. Known limitations of the IMS-TOF MS platforms that presently mitigate this attraction include the need for extensive signal averaging due to factors that include significant ion losses in the IMS-TOF interface and an ion utilization efficiency of less than ~1% with continuous ion sources (e.g. ESI). We have developed a new multiplexed ESI-IMS-TOF mass spectrometer that enables lossless ion transmission through the IMS-TOF as well as a utilization efficiency of >50% for ions from the ESI source. Initial results with a mixture of peptides show a ~10-fold increase in signal-to-noise ratio with the multiplexed approach compared to a signal averaging approach, with no reduction in either IMS or TOF MS resolution.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
902661
Report Number(s):
PNNL-SA-51865
20496; 400412000
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Analytical Chemistry, 79(6):2451-2462; Journal Volume: 79; Journal Issue: 6
Country of Publication:
United States
Language:
English
Subject:
Electrospray ionization, multiplexing, ion mobility spectrometry, time-of-flight mass spectrometry; Environmental Molecular Sciences Laboratory

Citation Formats

Belov, Mikhail E., Buschbach, Michael A., Prior, David C., Tang, Keqi, and Smith, Richard D.. Multiplexed Ion Mobility Spectrometry - Orthogonal Time-Of-Flight Mass Spectrometry. United States: N. p., 2007. Web. doi:10.1021/ac0617316.
Belov, Mikhail E., Buschbach, Michael A., Prior, David C., Tang, Keqi, & Smith, Richard D.. Multiplexed Ion Mobility Spectrometry - Orthogonal Time-Of-Flight Mass Spectrometry. United States. doi:10.1021/ac0617316.
Belov, Mikhail E., Buschbach, Michael A., Prior, David C., Tang, Keqi, and Smith, Richard D.. Thu . "Multiplexed Ion Mobility Spectrometry - Orthogonal Time-Of-Flight Mass Spectrometry". United States. doi:10.1021/ac0617316.
@article{osti_902661,
title = {Multiplexed Ion Mobility Spectrometry - Orthogonal Time-Of-Flight Mass Spectrometry},
author = {Belov, Mikhail E. and Buschbach, Michael A. and Prior, David C. and Tang, Keqi and Smith, Richard D.},
abstractNote = {Ion mobility spectrometry (IMS) coupled to orthogonal time-of-flight mass spectrometry (TOF) has shown significant promise for the characterization of complex biological mixtures. The enormous complexity of biological samples (e.g. from proteomics) and the need for both biological and technical analysis replicates imposes major challenges for multidimensional separation platforms in regard to both sensitivity and sample throughput. A major potential attraction of the IMS-TOF MS platform is separation speeds exceeding that of conventional condensed-phase separations by orders of magnitude. Known limitations of the IMS-TOF MS platforms that presently mitigate this attraction include the need for extensive signal averaging due to factors that include significant ion losses in the IMS-TOF interface and an ion utilization efficiency of less than ~1% with continuous ion sources (e.g. ESI). We have developed a new multiplexed ESI-IMS-TOF mass spectrometer that enables lossless ion transmission through the IMS-TOF as well as a utilization efficiency of >50% for ions from the ESI source. Initial results with a mixture of peptides show a ~10-fold increase in signal-to-noise ratio with the multiplexed approach compared to a signal averaging approach, with no reduction in either IMS or TOF MS resolution.},
doi = {10.1021/ac0617316},
journal = {Analytical Chemistry, 79(6):2451-2462},
number = 6,
volume = 79,
place = {United States},
year = {Thu Mar 15 00:00:00 EDT 2007},
month = {Thu Mar 15 00:00:00 EDT 2007}
}