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Title: Molecular cytogenetic characterization of a human thyroid cancercell line

Abstract

The incidence of papillary thyroid carcinoma (PTC) increases significantly after exposure of the head and neck region to ionizing radiation, yet we know neither the steps involved in malignant transformation of thyroid epithelium nor the specific carcinogenic mode of action of radiation. Such increased tumor frequency became most evident in children after the 1986 nuclear accident in Chernobyl, Ukraine. In the twelve years following the accident, the average incidence of childhood PTCs (chPTC) increased over one hundred-fold compared to the rate of about 1 tumor incidence per 10{sup 6} children per year prior to 1986. To study the etiology of radiation-induced thyroid cancer, we formed an international consortium to investigate chromosomal changes and altered gene expression in cases of post-Chernobyl chPTC. Our approach is based on karyotyping of primary cultures established from chPTC specimens, establishment of cell lines and studies of genotype-phenotype relationships through high resolution chromosome analysis, DNA/cDNA micro-array studies, and mouse xenografts that test for tumorigenicity. Here, we report the application of fluorescence in situ hybridization (FISH)-based techniques for the molecular cytogenetic characterization of a highly tumorigenic chPTC cell line, S48TK, and its subclones. Using chromosome 9 rearrangements as an example, we describe a new approach termed ''BAC-FISH''more » to rapidly delineate chromosomal breakpoints, an important step towards a better understanding of the formation of translocations and their functional consequences.« less

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
Sponsoring Org.:
USDOE Director. Office of Science. Office of Health andEnvironmental Research; National Institutes of Health Grants CA88258 andHD44313 and HD45736; European /community Grant F14PCT95000IL
OSTI Identifier:
901816
Report Number(s):
LBNL-59747
R&D Project: L0130; BnR: 400412000; TRN: US200715%%154
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Cytogenetics and Genome Research; Journal Volume: 114; Related Information: Journal Publication Date: 2006
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARCINOMAS; CHILDREN; CHROMOSOMES; EPITHELIUM; ETIOLOGY; FLUORESCENCE; FUNCTIONALS; GENES; IN-SITU HYBRIDIZATION; IONIZING RADIATIONS; NEOPLASMS; RESOLUTION; THYROID; TRANSFORMATIONS; UKRAINE

Citation Formats

Weier, Heinz-Ulrich G., Tuton, Tiffany B., Ito, Yuko, Chu, LisaW., Lu, Chung-Mei, Baumgartner, Adolf, Zitzelsberger, Horst F., and Weier,Jingly F. Molecular cytogenetic characterization of a human thyroid cancercell line. United States: N. p., 2006. Web. doi:10.1159/000094215.
Weier, Heinz-Ulrich G., Tuton, Tiffany B., Ito, Yuko, Chu, LisaW., Lu, Chung-Mei, Baumgartner, Adolf, Zitzelsberger, Horst F., & Weier,Jingly F. Molecular cytogenetic characterization of a human thyroid cancercell line. United States. doi:10.1159/000094215.
Weier, Heinz-Ulrich G., Tuton, Tiffany B., Ito, Yuko, Chu, LisaW., Lu, Chung-Mei, Baumgartner, Adolf, Zitzelsberger, Horst F., and Weier,Jingly F. Wed . "Molecular cytogenetic characterization of a human thyroid cancercell line". United States. doi:10.1159/000094215. https://www.osti.gov/servlets/purl/901816.
@article{osti_901816,
title = {Molecular cytogenetic characterization of a human thyroid cancercell line},
author = {Weier, Heinz-Ulrich G. and Tuton, Tiffany B. and Ito, Yuko and Chu, LisaW. and Lu, Chung-Mei and Baumgartner, Adolf and Zitzelsberger, Horst F. and Weier,Jingly F.},
abstractNote = {The incidence of papillary thyroid carcinoma (PTC) increases significantly after exposure of the head and neck region to ionizing radiation, yet we know neither the steps involved in malignant transformation of thyroid epithelium nor the specific carcinogenic mode of action of radiation. Such increased tumor frequency became most evident in children after the 1986 nuclear accident in Chernobyl, Ukraine. In the twelve years following the accident, the average incidence of childhood PTCs (chPTC) increased over one hundred-fold compared to the rate of about 1 tumor incidence per 10{sup 6} children per year prior to 1986. To study the etiology of radiation-induced thyroid cancer, we formed an international consortium to investigate chromosomal changes and altered gene expression in cases of post-Chernobyl chPTC. Our approach is based on karyotyping of primary cultures established from chPTC specimens, establishment of cell lines and studies of genotype-phenotype relationships through high resolution chromosome analysis, DNA/cDNA micro-array studies, and mouse xenografts that test for tumorigenicity. Here, we report the application of fluorescence in situ hybridization (FISH)-based techniques for the molecular cytogenetic characterization of a highly tumorigenic chPTC cell line, S48TK, and its subclones. Using chromosome 9 rearrangements as an example, we describe a new approach termed ''BAC-FISH'' to rapidly delineate chromosomal breakpoints, an important step towards a better understanding of the formation of translocations and their functional consequences.},
doi = {10.1159/000094215},
journal = {Cytogenetics and Genome Research},
number = ,
volume = 114,
place = {United States},
year = {Wed Jan 04 00:00:00 EST 2006},
month = {Wed Jan 04 00:00:00 EST 2006}
}