skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Microfluidic polymeric constant flow-through mRNA sample preconcentrator.


No abstract prepared.

; ; ;
Publication Date:
Research Org.:
Sandia National Laboratories
Sponsoring Org.:
OSTI Identifier:
Report Number(s):
TRN: US200714%%10
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proposed for publication in Analytical Chemistry.
Country of Publication:
United States

Citation Formats

Shepodd, Timothy J., Satterfield, Brent, Hukari, Kyle W., and West, Jason A. A. Microfluidic polymeric constant flow-through mRNA sample preconcentrator.. United States: N. p., 2006. Web.
Shepodd, Timothy J., Satterfield, Brent, Hukari, Kyle W., & West, Jason A. A. Microfluidic polymeric constant flow-through mRNA sample preconcentrator.. United States.
Shepodd, Timothy J., Satterfield, Brent, Hukari, Kyle W., and West, Jason A. A. Sun . "Microfluidic polymeric constant flow-through mRNA sample preconcentrator.". United States. doi:.
title = {Microfluidic polymeric constant flow-through mRNA sample preconcentrator.},
author = {Shepodd, Timothy J. and Satterfield, Brent and Hukari, Kyle W. and West, Jason A. A.},
abstractNote = {No abstract prepared.},
doi = {},
journal = {Proposed for publication in Analytical Chemistry.},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
  • A novel and versatile processing method was developed for the formation of gel scaffolds with in-situ AChE-AuNPs immobilization for biosensing of organophosphorus compounds. The biosensor designed by our new approach shows high sensitivity, selectivity and reactivation efficiency. This flow induced immobilziation technique opens up new pathways for designing simple, fast, biocompatible, and cost-effective process for enhanced sensor performance and on-site testing of a variety of toxic organophosphorus compounds.
  • Here, a major advantage of microfluidic devices is the ability to manipulate small sample volumes, thus reducing reagent waste and preserving precious sample. However, to achieve robust sample manipulation it is necessary to address device integration with the macroscale environment. To realize repeatable, sensitive particle separation with microfluidic devices, this protocol presents a complete automated and integrated microfluidic platform that enables precise processing of 0.15–1.5 ml samples using microfluidic devices. Important aspects of this system include modular device layout and robust fixtures resulting in reliable and flexible world to chip connections, and fully-automated fluid handling which accomplishes closed-loop sample collection,more » system cleaning and priming steps to ensure repeatable operation. Different microfluidic devices can be used interchangeably with this architecture. Here we incorporate an acoustofluidic device, detail its characterization, performance optimization, and demonstrate its use for size-separation of biological samples. By using real-time feedback during separation experiments, sample collection is optimized to conserve and concentrate sample. Although requiring the integration of multiple pieces of equipment, advantages of this architecture include the ability to process unknown samples with no additional system optimization, ease of device replacement, and precise, robust sample processing.« less
  • A large-sample-volume constant-flow magic angle sample spinning (CF-MAS) NMR probe is reported for in-situ studies of the reaction dynamics, stable intermediates/transition states, and mechanisms of catalytic reactions. In our approach, the reactants are introduced into the catalyst bed using a fixed tube at one end of the MAS rotor while a second fixed tube, linked to a vacuum pump, is attached at the other end of the rotor. The pressure difference between both ends of the catalyst bed inside the sample cell space forces the reactants flowing through the catalyst bed, which improves the diffusion of the reactants and products.more » This design allows the use of a large sample volume for enhanced sensitivity and thus permitting in-situ 13C CF-MAS studies at natural abundance. As an example of application, we show that reactants, products and reaction transition states associated with the 2-butanol dehydration reaction over a mesoporous silicalite supported heteropoly acid catalyst (HPA/meso-silicalite-1) can all be detected in a single 13C CF-MAS NMR spectrum at natural abundance. Coke products can also be detected at natural 13C abundance and under the stopped flow condition. Furthermore, 1H CF-MAS NMR is used to identify the surface functional groups of HPA/meso-silicalite-1 under the condition of in-situ drying . We also show that the reaction dynamics of 2-butanol dehydration using HPA/meso-silicalite-1 as a catalyst can be explored using 1H CF-MAS NMR.« less
  • The first commercial use of a polymeric drag-reducing additive to increase the flow rate in a crude oil pipeline began during 1979 in the Trans Alaska Pipeline System (TAPS). This study discusses the details of the two drag-reduction experiments conducted in TAPS that ultimately led to the decision for full-scale additive use. The logistics pertaining to and the benefits resulting from the current application also are discussed. 15 refs.
  • BS>An analysis is made of nonstationary flow of conducting, viscous, incompressible fluid in an infinitely long tube in an external, uniform, transverse magnetic field. The general aspects of flow in tubes of arbitrary cross section, with conducting or non-conducting walls and mixed boundary conditions, and in round tubes with non-conducting walls are analyzed. (R.V.J.)